The use of ubiquitin lysine mutants to characterize E2-E3 linkage specificity: Mass spectrometry offers a cautionary "tail"

Proteomics

Volumn 15, Issue 17, 2015, Pages 2910-2915

  Hong, Jenny H ^a   Ng, Deborah ^a   Srikumar, Tharan ^a   Raught, Brian ^a  

^a PRINCESS MARGARET HOSPITAL   (Canada)

Author keywords

Mass spectrometry; Spectral matching; Technology; Ubiquitin; Ubiquitin chains; Ubiquitin linkages

Indexed keywords

ANTIBODY; ARGININE; EPITOPE; LYSINE; MUTANT PROTEIN; PROTEIN VARIANT; UBIQUITIN; UBIQUITIN PROTEIN LIGASE; UBIQUITIN PROTEIN LIGASE E2; UBIQUITIN PROTEIN LIGASE E3; UNCLASSIFIED DRUG; CDC34 PROTEIN, HUMAN; UBE2D2 PROTEIN, HUMAN; UBE2E2 PROTEIN, HUMAN; UBE2S PROTEIN, HUMAN; UBIQUITIN CONJUGATING ENZYME;

AMINO ACID SEQUENCE; ARTICLE; ELECTROSPRAY; HUMAN; IN VITRO STUDY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN STRUCTURE; TANDEM MASS SPECTROMETRY; UBIQUITINATION; WESTERN BLOTTING; X RAY FILM; AMINO ACID SUBSTITUTION; CHEMISTRY; GENETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCEDURES;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; LYSINE; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; MUTATION; TANDEM MASS SPECTROMETRY; UBIQUITIN; UBIQUITIN-CONJUGATING ENZYMES; UBIQUITIN-PROTEIN LIGASES; UBIQUITINATION;

EID: 84940595583     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.201500058     Document Type: Article

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