The efficacy of uracil DNA glycosylase pretreatment in amplicon-based massively parallel sequencing with DNA extracted from archived formalin-fixed paraffin-embedded esophageal cancer tissues

Cancer Genetics

Volumn 208, Issue 9, 2015, Pages 415-427

  Serizawa, Masakuni ^a   Yokota, Tomoya ^a   Hosokawa, Ayumu ^b   Kusafuka, Kimihide ^a   Sugiyama, Toshiro ^b   Tsubosa, Yasuhiro ^a   Yasui, Hirofumi ^a   Nakajima, Takashi ^a   Koh, Yasuhiro ^a,c  

^a SHIZUOKA CANCER CENTER   (Japan)

^b TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY   (Japan)

^c WAKAYAMA MEDICAL UNIVERSITY   (Japan)

Author keywords

Amplicon based massively parallel sequencing; Cytosine deamination; Formalin fixed paraffin embedded samples; Molecular cancer diagnostics; Uracil DNA glycosylase

Indexed keywords

ADENINE; CYTOSINE; DNA; FORMALDEHYDE; FORMALIN FIXED PARAFFIN EMBEDDED DNA; GUANINE; PARAFFIN; THYMINE; UNCLASSIFIED DRUG; URACIL DNA GLYCOSIDASE;

AMPLICON; ARTICLE; CANCER TISSUE; CONTROLLED STUDY; DNA DETERMINATION; DNA EXTRACTION; DNA FRAGMENTATION; DNA SEQUENCE; ESOPHAGUS CANCER; GENETIC VARIABILITY; HUMAN; HUMAN TISSUE; MASSIVELY PARALLEL SEQUENCING; PRIORITY JOURNAL; SINGLE NUCLEOTIDE POLYMORPHISM; SINGLE NUCLEOTIDE VARIANT; ESOPHAGUS TUMOR; GENETICS; HIGH THROUGHPUT SEQUENCING; METABOLISM; MUTATION; POLYMERASE CHAIN REACTION; PROCEDURES; TISSUE FIXATION;

DNA; ESOPHAGEAL NEOPLASMS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; MUTATION; PARAFFIN EMBEDDING; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; TISSUE FIXATION; URACIL-DNA GLYCOSIDASE;

EID: 84940451367     PISSN: 22107762     EISSN: 22107770     Source Type: Journal    
DOI: 10.1016/j.cancergen.2015.05.001     Document Type: Article

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