Multi-step preparation technique to recover multiple metabolite compound classes for in-depth and informative metabolomic analysis

Journal of Visualized Experiments

Volumn , Issue 89, 2014, Pages

  Cruickshank Quinn, Charmion ^a   Quinn, Kevin D ^a   Powell, Roger ^a   Yang, Yanhui ^a   Armstrong, Michael ^a   Mahaffey, Spencer ^b   Reisdorph, Richard ^a   Reisdorph, Nichole ^a  

^a DEPARTMENT OF MEDICINE   (United States)

^b UNIVERSITY OF COLORADO SCHOOL OF MEDICINE   (United States)

Author keywords

Analytical; Bioengineering; Bronchoalveolar lavage fluid; Cerebrospinal fluid; Chemistry techniques; Fluids and secretions; Issue 89; Lipids; Liquid chromatography; Liquid liquid extraction; Mass spectrometry; Metabolomics; Plasma; Profiling; Small molecules; Solid phase extraction

Indexed keywords

EID: 84940270333     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51670     Document Type: Article

References (26)

1. Beckonert, O. et al. Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts. Nature Protocols. 2, 2692-2703 (2007).
2. Clarke, C. J., & Haselden, J. N. Metabolic Profiling as a Tool for Understanding Mechanisms of Toxicity. Toxicologic Pathology. 36, 140-147, doi:10.1177/0192623307310947 (2008).
3. Wang, X., Zhang, A., & Sun, H. Power of Metabolomics in Diagnosis and Biomarker Discovery of Hepatocellular Carcinoma. Hepatology. 57, 2072-2077 (2013).
4. Collino, S., Martin, F.-P. J., Kochhar, S., & Rezzi, S. Monitoring Healthy Metabolic Trajectories with Nutritional Metabonomics. Nutrients. 1, 101-110, doi:10.3390/nu1010101 (2009).
5. Cevallos-Cevallos, J. M., Reyes-De-Corcuera, J. I., Etxeberria, E., Danyluk, M. D., & Rodrick, G. E. Metabolomic analysis in food science: a review. Trends in Food Scienc., & Technology. 20, 557-566 (2009).
6. Nicholson, J. K., Connelly, J., Lindon, J. C., & Holmes, E. Metabonomics: a platform for studying drug toxicity and gene function. Nature reviews. Drug Discovery. 1, 153-161, doi:10.1038/nrd728 (2002).
7. Wishart, D. et al. in Metabolome Analysis: An Introduction 253-288 John Wile., & Sons, Inc. (2006).
8. Vuckovic, D. Current trends and challenges in sample preparation for global metabolomics using liquid chromatography-mass spectrometry. Analytical and Bioanalytical Chemistry. 403, 1523-1548 (2012).
9. Bollard, M. E., Stanley, E. G., Lindon, J. C., Nicholson, J. K., & Holmes, E. NMR-based metabonomic approaches for evaluating physiological influences on biofluid composition. NMR in Biomedicine. 18, 143-162 (2005).
10. Dong, M. W. Modern HPLC for Practicing Scientists. 286 John Wile., & Sons, Inc. (2006).
11. Want, E. J. et al. Solvent-dependent metabolite distribution, clustering, and protein extraction for serum profiling with mass spectrometry. Analytical Chemistry. 78, 743-752 (2006).
12. Yang, Y. et al. New sample preparation approach for mass spectrometry-based profiling of plasma results in improved coverage of metabolome. Journal of Chromatography A. 1300, 217-226, doi:10.1016/j.chroma.2013.04.030 (2013).
13. Folch, J., Lees, M., & Stanley, G. H. S. A simple method for the isolation and purification of total lipides from animal tissues. The Journal of Biological Chemistry. 226, 497-509 (1957).
14. Matyash, V., Liebisch, G., Kurzchalia, T. V., Shevchenko, A., & Schwudke, D. Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics. Journal of Lipid Research. 49, 1137-1146 (2008).
15. Bligh, E. G., & Dyer, W. J. A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology. 37, 911-917 (1957).
16. Ferreiro-Vera, C., Priego-Capote, F., & Luque de Castro, M. D. Comparison of sample preparation approaches for phospholipids profiling in human serum by liquid chromatography-tandem mass spectrometry. Journal of Chromatography A. 1240, 21-28 (2012).
17. Michopoulos, F., Lai, L., Gika, H., Theodoridis, G., & Wilson, I. UPLC-MS-Based Analysis of Human Plasma for Metabonomics Using Solvent Precipitation or Solid Phase Extraction. Journal of Proteome Research. 8, 2114-2121, doi:10.1021/pr801045q (2009).
18. Kerns, E. H., & Di, L. Drug-like Properties: Concepts, Structure Design and Methods: from ADME to toxicity optimization. First edn, Elsevier Academic Press (2008).
19. Manach, C., Scalbert, A., Morand, C., Rémésy, C., & Jiménez, L. Polyphenols: food sources and bioavailability. American Journal of Clinical Nutrition. 79, 727-747 (2004).
20. Weimer, J. M., Kriscenski-Perry, E., Elshatory, Y., & Pearce, D. A. The neuronal ceroid lipofuscinoses. Mutations in different proteins result in similar disease. NeuroMolecular Medicine. 1, 111-124 (2002).
21. Schulze, H., & Sandhoff, K. Lysosomal lipid storage diseases. Cold Spring Harbor Perspectives in Biology. 3, a004804/004801-a004804/004819 (2011).
22. Kuhn, E, et al. Interlaboratory evaluation of automated, multiplexed peptide immunoaffinity enrichment coupled to multiple reaction monitoring mass spectrometry for quantifying proteins in plasma. Molecular and Cellular Proteomics. 11, doi:10.1074/mcp.M111.013854 (2012).
23. Rist, M. J, et al. Influence of Freezing and Storage Procedure on Human Urine Samples in NMR-Based Metabolomics. Metabolites. 3, 243-258, doi:10.3390/metabo3020243 (2013).
24. Boomsma, F., Alberts, G., van Eijk, L., Manin Veld, A. J., & Schalekamp, M. A. Optimal Collection and Storage Conditions for Catecholamine Measurements in Human Plasma and Urine. Clinical Chemistry. 39, 2503-2508 (1993).
25. Wood, J. T. et al. Comprehensive profiling of the human circulating endocannabinoid metabolome: clinical sampling and sample storage parameters. Clinical Chemistry and Laboratory Medicine. 46, 1289-1295, doi:10.1515/CCLM.2008.242 (2008).
26. Bahr, T. M. et al. Peripheral blood mononuclear cell gene expression in chronic obstructive pulmonary disease. American Journal of Respiratory Cell and Molecular Biology. 49, 316-323, doi:10.1165/rcmb.2012-0230OC (2013).