Compartmentalized self-tagging for in vitro-directed evolution of XNA polymerases

Current Protocols in Nucleic Acid Chemistry

Volumn 2014, Issue , 2014, Pages 9.9.1-9.9.18

  Pinheiro, Vitor B ^a,b   Arangundy Franklin, Sebastian ^c   Holliger, Philipp ^c  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF LONDON   (United Kingdom)

^c MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

Compartmentalized self tagging; CST; DNA polymerases; In vitro directed evolution; Synthetic nucleic acids; XNA

Indexed keywords

DNA POLYMERASE; PLASMID DNA; UNCLASSIFIED DRUG; XENOBIOTIC NUCLEIC ACID POLYMERASE; DNA PRIMASE; NUCLEIC ACID; XENOBIOTIC AGENT;

ARTICLE; COMPARTMENTALIZED SELF TAGGING; EMULSION; ENZYME SPECIFICITY; ESCHERICHIA COLI; EVOLUTION; GENETIC PROCEDURES; GENOTYPE PHENOTYPE CORRELATION; IN VITRO DIRECTED EVOLUTION; NONHUMAN; NUCLEIC ACID SYNTHESIS; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; THERMOCOCCUS; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; PROCEDURES; SYNTHESIS;

DIRECTED MOLECULAR EVOLUTION; DNA PRIMASE; NUCLEIC ACIDS; XENOBIOTICS;

EID: 84939965847     PISSN: 19349270     EISSN: 19349289     Source Type: Journal    
DOI: 10.1002/0471142700.nc0909s57     Document Type: Article

References (15)

1. Boudou, V., Kerremans, L., De Bouvere, B., Lescrinier, E., Schepers, G., Busson, R., Van Aerschot, A., and Herdewijn, P. 1999. Base pairing of anhydrohexitol nucleosides with 2, 6-diaminopurine, 5-methylcytosine and uracil as base moiety. Nucleic Acids Res. 27:1450-1456.
2. Burmeister, P.E., Lewis, S.D., Silva, R.F., Preiss, J.R., Horwitz, L.R., Pendergrast, P.S., Mc-Cauley, T.G., Kurz, J.C., Epstein, D.M., Wilson, C., and Keefe, A.D. 2005. Direct in vitro selection of a 2'-O-methyl aptamer to VEGF. Chem. Biol. 12:25-33.
3. Desjardins, P.R. and Conklin, D.S. 2011. Microvolume quantitation of nucleic acids. Curr. Protoc. Mol. Biol. 93:A.3J.1-A.3J.16.
4. Diehl, F., Li, M., He, Y., Kinzler, K.W., Vogelstein, B., and Dressman, D. 2006. BEAMing: Singlemolecule PCR on microparticles in water-in-oil emulsions. Nat. Methods 3:551-559.
5. Engler, C., Kandzia, R., and Marillonnet, S. 2008. A one pot, one step, precision cloning method with high-throughput capability. PloS One 3:e3647.
6. Ghadessy, F.J., Ramsay, N., Boudsocq, F., Loakes, D., Brown, A., Iwai, S., Vaisman, A., Woodgate, R., and Holliger, P. 2004. Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution. Nat. Biotechnol. 22:755-759.
7. Jager, S. and Famulok, M. 2004. Generation and enzymatic amplification of high-density functionalized DNA double strands. Angew Chem. Int. Ed. Engl. 43:3337-3340.
8. Loakes, D. and Holliger, P. 2009. Polymerase engineering: Towards the encoded synthesis of unnatural biopolymers. Chem. Commun. 31:4619-4631.
9. Ochman, H., Gerber, A.S., and Hartl, D.L. 1988. Genetic applications of an inverse polymerase chain reaction. Genetics 120:621-623.
10. Pinheiro, V.B., Ong, J.L., and Holliger, P. 2012. Polymerase engineering: From PCR and sequencing to synthetic biology. In Protein Engineering HandbookVol. III (S. Lutz&U.T. Bornscheuer, eds.) pp. 279-302. Wiley-VCH. Weinheim, Germany.
11. Pinheiro, V.B., Taylor, A.I., Cozens, C., Abramov, M., Renders, M., Zhang, S., Chaput, J.C., Wengel, J., Peak-Chew, S.Y., McLaughlin, S.H., Herdewijn, P., and Holliger, P. 2012. Synthetic genetic polymers capable of heredity and evolution. Science 336:341-344.
12. Seidman, C.E., Struhl, K., Sheen, J., and Jessen, T. 1997. Introduction of plasmid DNA into cells. Curr. Protoc. Mol. Biol. 37:1.8.1-1.8.10.
13. Skerra, A. 1994. Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli. Gene 151:131-135.
14. Vant-Hull, B., Gold, L., and Zichi, D.A. 2000. Theoretical principles of in vitro selection using combinatorial nucleic acid libraries. Curr. Protoc. Nucl. Acid. Chem. 0:9.1.1-9.1.16.
15. Voytas, D. 2000. Agarose gel electrophoresis. Curr. Protoc. Mol. Biol. 51:2.5A.1-2.5A.9.