Comparison of real-time PCR methods for the detection of Naegleria fowleri in surface water and sediment

Parasitology Research

Volumn 114, Issue 5, 2015, Pages 1739-1746

  Streby, Ashleigh ^a   Mull, Bonnie J ^b   Levy, Karen ^a   Hill, Vincent R ^c  

^a EMORY UNIVERSITY   (United States)

^b FLORIDA DEPARTMENT OF HEALTH   (United States)

^c CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

Author keywords

Environmental detection; Method comparison; Naegleria fowleri; Real time PCR

Indexed keywords

PROTOZOAL DNA; WATER;

GENETICS; GENOTYPE; HUMAN; ISOLATION AND PURIFICATION; NAEGLERIA FOWLERI; PARASITOLOGY; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

DNA, PROTOZOAN; GENOTYPE; HUMANS; NAEGLERIA FOWLERI; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; WATER;

EID: 84939946082     PISSN: 09320113     EISSN: 14321955     Source Type: Journal    
DOI: 10.1007/s00436-015-4359-5     Document Type: Article

References (26)

1. Ahmad AF, Lonnen J, Andrew PW, Kilvington S (2011) Development of a rapid DNA extraction method and one-step nested PCR for the detection of Nagleria fowleri from the environment. Water Res 45:5211–5217
2. Behets J, Declerck P, Delaedt Y, Verelst L, Ollevier F (2006) Quantitative detection and differentiation of free-living amoeba species using SYBR Green-based real-time PCR melting curve analysis. Curr Microbiol 53:506–509
3. Behets J, Declerck P, Delaedt Y, Verelst L, Ollevier F (2007) A duplex real-time PCR assay for the quantitative detection of Naegleria fowleri in water samples. Water Res 41:118–126
4. De Jonckheere JF (2002) A century of research on the amoeboflagellate genus Naegleria. Acta Protozool 41(4):309–342
5. DeJonckheere JF (2011) Origin and evolution of the worldwide distributed pathogenic amoeboflagellate Naegleria fowleri. Infect Genet Evol 11:1520–1528
6. Griffin JL (1972) Temperature Tolerance of Pathogenic and Nonpathogenic Free-Living Amoebas. Science 178(4063):869–870
7. Hill VR, Kahler AM, Jothikumar N, Johnson TB, Hahn D, Cromeans TL (2007) Multistate evaluation of an ultrafiltration-based procedure for simultaneous recovery of enteric microbes in 100-liter tap water samples. Appl Environ Microbiol 73(13):4218–4225
8. HillVR, Jothikumar N, Vinje J, Cromeans TL (2010) Sample Preparation Methods for Molecular Techniques for Drinking Water. Project Report #3108, Water Research Foundation
9. Jiang JL, Alderisio KA, Singh A, Xiao LH (2005) Development of Procedures for Direct Extraction of Cryptosporidium DNA from Water Concentrates and for Relief of PCR Inhibitors. Appl Environ Microbiol 71(3):1135–1141
10. John DT (1982) Primary Amebic Meningoencephalitis and the biology of Naegleria fowleri. Annu Rev Microbiol 36:101–123
11. Lares-Villa F, Hernández-Peña C (2010) Concentration of Naegleria fowleri in natural waters used for recreational purposes in Sonora, Mexico (November 2007-October 2008). Exp Parasitol 126:33–36
12. Marciano-Cabral F (1988) Biology of Naegleria spp. Microbiol Rev 52(1):114–133
13. Marciano-Cabral F, Cabral GA (2007) The immune response to Naegleria fowleri amebae and pathogenesis of infection. FEMS Immunol Med Microbiol 51:243–259
14. Marciano-Cabral F, MacLean R, Mensah A, LaPat-Polasko L (2003) Identification of Naegleria fowleri in domestic water sources by nested PCR. Appl Environ Microbiol 69(10):5864–5869
15. Mull B, Narayanan J, Hill VR (2013) Improved method for the detection and quantification of Naegleria fowleri in water and sediment using immunomagnetic separation and real-time PCR. J Parasitol Res 2013
16. Natural Resources Conservation Service (2013) Distribution Maps of Dominant Soil Orders. Accessed 11/06/2013, from http://soils.usda.gov/technical/classification/orders/
17. Painter SM, Pfau RS, Brady JA, McFarland AM (2013) Quantitative assessment of Naegleria fowleri and Escherichia coli concentrations within a Texas reservoir. J Water Health 11(2):346–357
18. Puzon GJ, Lancaster JA, Wylie JT, Plumb JA (2009) Rapid detection of Naegleria fowleri in water distribution pipeline biofilms and drinking water samples. Environ Sci Technol 43:6691–6696
19. Qvarnstrom Y, Visvesvara GS, Sriram R, da Silva AJ (2006) Multiplex real-time PCR assay for simultaneous detection of Acanthamoeba spp., Balamuthia mandrillaris, and Naegleria fowleri. J Clin Microbiol 44(10):3589–3595
20. Réveiller FL, Cabanes PA, Marciano-Cabral F (2002) Development of a nested PCR assay to detect the pathogenic free-living amoeba Naegleria fowleri. Parasitol Res 88:443–450
21. Robinson BS, Monis PT, Dobson PJ (2006) Rapid, sensitive, and discriminating identification of Naegleria spp. by real-time PCR and melting-curve analysis. Appl Environ Microbiol 72(9):5857–5863
22. Visvesvara GS, Balamuth W (1975) Comparative studies on related free-living and pathogenic amebae with special reference to Acanthamoeba. J Protozool 22(2):245–256
23. Visvesvara GS, Moura H, Schuster FL (2007) Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea. FEMS Immunol Med Microbiol 50:1–26
24. Wilson IG (1997) Inhibition and facilitation of nucleic acid amplification. Appl Environ Microbiol 63(10):3741–3751
25. Yoder JS, Eddy BA, Visvesvara GS, Capewell L, Beach MJ (2010) The epidemiology of primary amoebic meningoencephalitis in the USA, 1962-2008. Epidemiol Infect 138(7):968–975
26. Yoder JS, Straif-Bourgeois S, Roy SL, Moore TA, Visvesvara GS, Ratard RC, Hill VR, Wilson JD, Linscott AJ, Crager R, Kozak NA, Sriram R, Narayanan J, Mull B, Kahler AM, Schneeberger C, da Silva AJ, Poudel M, Baumgarten KL, Xiao L, Beach MJ (2012) Primary amebic meningoencephalitis deaths associated with sinus irrigation using contaminated tap water. Clin Infect Dis 55(9):e79–e85