Development of a rapid DNA extraction method and one-step nested PCR for the detection of Naegleria fowleri from the environment

Water Research

Volumn 45, Issue 16, 2011, Pages 5211-5217

  Ahmad, Arine Fadzlun ^a,b   Lonnen, James ^a   Andrew, Peter W ^a   Kilvington, Simon ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

^b UNIVERSITY OF MALAYA   (Malaysia)

Author keywords

Detection; DNA extraction; Ecology; Naegleria fowleri; Nested PCR; PAM

Indexed keywords

AQUATIC ORGANISMS; DNA; EXTRACTION; NUCLEAR FUELS; POLYMERASE CHAIN REACTION; WATER POLLUTION;

AQUATIC HABITATS; DETECTION; DNA EXTRACTION; EXTRACTION METHOD; FREE LIVINGS; NAEGLERIUM FOWLERI; NESTED PCR; PRIMARY AMEBIC MENINGOENCEPHALITIS; THERMAL; THERMOPHILICS;

NUCLEAR POWER PLANTS;

DNA; RIVER WATER;

BATHING WATER; DISEASE SPREAD; EUKARYOTE; INFECTIOUS DISEASE; NUCLEAR POWER PLANT; PATHOGENICITY; POLYMERASE CHAIN REACTION; PUBLIC HEALTH; RIVER POLLUTION; SAMPLING; THERMOPHILY;

ACANTHAMOEBA; AMOEBA (LIFE CYCLE STAGE); ARTICLE; CONTROLLED STUDY; DNA EXTRACTION; ENVIRONMENT; FRANCE; HARTMANNELLA; MICROORGANISM DETECTION; NAEGLERIA FOWLERI; NONHUMAN; NUCLEAR POWER PLANT; ONE STEP NESTED POLYMERASE CHAIN REACTION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RELIABILITY; SEDIMENT; SENSITIVITY ANALYSIS; THERMOPHILE; UNITED KINGDOM; VAHLKAMPFIA;

FRANCE; UNITED KINGDOM;

MASTIGOPHORA (FLAGELLATES); NAEGLERIA; NAEGLERIA FOWLERI;

EID: 80052264768     PISSN: 00431354     EISSN: 18792448     Source Type: Journal    
DOI: 10.1016/j.watres.2011.07.025     Document Type: Article

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