A spindle pole antigen gene MoSPA2 is important for polar cell growth of vegetative hyphae and conidia, but is dispensable for pathogenicity in Magnaporthe oryzae

Current Genetics

Volumn 60, Issue 4, 2014, Pages 255-263

  Li, Chao ^a   Yang, Jun ^a   Zhou, Wei ^a   Chen, Xiao Lin ^a   Huang, Jin Guang ^a   Cheng, Zhi Hua ^a   Zhao, Wen Sheng ^a   Zhang, Yan ^a   Peng, You Liang ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Morphogenesis; Polarisome; Polarized growth; The rice blast fungus; Vegetative hyphal growth

Indexed keywords

FUNGAL PROTEIN; FUNGUS ANTIGEN;

CELL POLARITY; FUNGUS HYPHAE; FUNGUS SPORE; GENE DELETION; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; IMMUNOLOGY; MAGNAPORTHE; METABOLISM; MICROBIOLOGY; ORYZA; PATHOGENICITY; PHENOTYPE; PLANT DISEASE; PLANT LEAF; SPINDLE POLE; VIRULENCE;

ANTIGENS, FUNGAL; CELL POLARITY; FUNGAL PROTEINS; GENE DELETION; GENE EXPRESSION REGULATION, FUNGAL; HYPHAE; MAGNAPORTHE; MUTAGENESIS, INSERTIONAL; ORYZA; PHENOTYPE; PLANT DISEASES; PLANT LEAVES; SEQUENCE DELETION; SPINDLE POLES; SPORES, FUNGAL; VIRULENCE;

EID: 84939889558     PISSN: 01728083     EISSN: 14320983     Source Type: Journal    
DOI: 10.1007/s00294-014-0431-4     Document Type: Article

References (35)

1. Amberg DC, Zahner JE, Mulholland JW, Pringle JR, Botstein D (1997) Aip3p/Bud6p, a yeast actin-interacting protein that is involved in morphogenesis and the selection of bipolar budding sites. Mol Bio Cell 8:729–753
2. Carbo N, Perez-Martin J (2008) Spa2 is required for morphogenesis but it is dispensable for pathogenicity in the phytopathogenic fungus Ustilago maydis. Fungal Genet Biol 45:1315–1327
3. Crampin H, Finley K, Gerami-Nejad M, Court H, Gale C, Berman J, Sudbery P (2005) Candida albicans hyphae have a Spitzenkorper that is distinct from the polarisome found in yeast and pseudohyphae. J Cell Sci 118:2935–2947
4. Delgehyr N, Lopes CS, Moir CA, Huisman SM, Segal M (2008) Dissecting the involvement of formins in Bud6p-mediated cortical capture of microtubules in S. cerevisiae. J Cell Sci 121:3803–3814
5. Du Y, Shi Y, Yang J, Chen X, Xue M, Zhou W, Peng YL (2013) A serine/threonine-protein phosphatase PP2A catalytic subunit is essential for asexual development and plant infection in Magnaporthe oryzae. Curr Genet 59:33–41
6. Gehrung S, Snyder M (1990) The SPA2 gene of Saccharomyces cerevisiae is important for pheromone-induced morphogenesis and efficient mating. J Cell Biol 111:1451–1464
7. Gouet P, Courcelle E, Stuart DI, Metoz F (1999) ESPript: analysis of multiple sequence alignments in PostScript. Bioinformatics 15:305–308
8. Harris SD (2006) Cell polarity in filamentous fungi: shaping the mold. Int Rev Cytol 251:41–77
9. Harris SD (2008) Branching of fungal hyphae: regulation, mechanisms and comparison with other branching systems. Mycologia 100:823–832
10. Harris SD (2009) The Spitzenkorper: a signalling hub for the control of fungal development? Mol Microbiol 73:733–736
11. Jeon J, Park SY, Chi MH, Choi J, Park J, Rho HS, Kim S, Goh J, Yoo S, Choi J, Park JY, Yi M, Yang S, Kwon MJ, Han SS, Kim BR, Khang CH, Park B, Lim SE, Jung K, Kong S, Karunakaran M, Oh HS, Kim H, Kim S, Park J, Kang S, Choi WB, Kang S, Lee YH (2007) Genome-wide functional analysis of pathogenicity genes in the rice blast fungus. Nat Genet 39:561–565
12. Jones LA, Sudbery PE (2010) Spitzenkorper, exocyst, and polarisome components in Candida albicans hyphae show different patterns of localization and have distinct dynamic properties. Eukaryot Cell 9:1455–1465
13. Knechtle P, Dietrich F, Philippsen P (2003) Maximal polar growth potential depends on the polarisome component AgSpa2 in the filamentous fungus Ashbya gossypii. Mol Biol Cell 14:4140–4154
14. Kohli M, Galati V, Boudier K, Roberson RW, Philippsen P (2008) Growth-speed-correlated localization of exocyst and polarisome components in growth zones of Ashbya gossypii hyphal tips. J Cell Sci 121:3878–3889
15. Lichius A, Yanez-Gutierrez ME, Read ND, Castro-Longoria E (2012) Comparative live-cell imaging analyses of SPA-2, BUD-6 and BNI-1 in Neurospora crassa reveal novel features of the filamentous fungal polarisome. PLoS ONE 7:e30372
16. Lu S, Lyngholm L, Yang G, Bronson C, Yoder OC, Turgeon BG (1994) Tagged mutations at the Tox1 locus of Cochliobolus heterostrophus by restriction enzyme-mediated integration. Proc Natl Acad Sci 91:12649–12653
17. Meyer V, Arentshorst M, van den Hondel CA, Ram AF (2008) The polarisome component SpaA localises to hyphal tips of Aspergillus niger and is important for polar growth. Fungal Genet Biol 45:152–164
18. Park G, Bruno KS, Staiger CJ, Talbot NJ, Xu JR (2004) Independent genetic mechanisms mediate turgor generation and penetration peg formation during plant infection in the rice blast fungus. Mol Microbiol 53:1695–1707
19. Patkar RNI, Suresh A, Naqvi NI (2010) MoTea4-mediated polarized growth is essential for proper asexual development and pathogenesis in Magnaporthe oryzae. Eukaryot Cell 9:1029–1038
20. Peng YL, Shishiyama J (1988) Temporal sequence of cytological events in rice leaves infected with Pyricularia oryzae. Can J Bot 66:730–735
21. Roemer T, Vallier L, Sheu YJ, Snyder M (1998) The Spa2-related protein, Sph1p, is important for polarized growth in yeast. J Cell Sci 111(Pt 4):479–494
22. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
23. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
24. Sheu YJ, Santos B, Fortin N, Costigan C, Snyder M (1998) Spa2p interacts with cell polarity proteins and signaling components involved in yeast cell morphogenesis. Mol Cell Biol 18:4053–4069
25. Shimizu J, Yoda K, Yamasaki M (1994) The hypo-osmolarity-sensitive phenotype of the Saccharomyces cerevisiae hpo2 mutant is due to a mutation in PKC1, which regulates expression of beta-glucanase. Mol Gen Genet 242:641–648
26. Sweigard JA, Carroll AM, Farrall L, Chumley FG, Valent B (1998) Magnaporthe grisea pathogenicity genes obtained through insertional mutagenesis. Mol Plant Microbe Interact 11:404–412
27. Talbot NJ, Kershaw MJ, Wakley GE, De Vries O, Wessels J, Hamer JE (1996) MPG1 encodes a fungal hydrophobin involved in surface interactions during infection-related development of Magnaporthe grisea. Plant Cell 8:985–999
28. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599
29. Tcheperegine SE, Gao XD, Bi E (2005) Regulation of cell polarity by interactions of Msb3 and Msb4 with Cdc42 and polarisome components. Mol Cell Biol 25:8567–8580
30. Valtz N, Herskowitz I (1996) Pea2 protein of yeast is localized to sites of polarized growth and is required for efficient mating and bipolar budding. J Cell Biol 135:725–739
31. van Drogen F, Peter M (2002) Spa2p functions as a scaffold-like protein to recruit the Mpk1p MAP kinase module to sites of polarized growth. Curr Biol 12:1698–1703
32. Virag A, Harris SD (2006) Functional characterization of Aspergillus nidulans homologues of Saccharomyces cerevisiae Spa2 and Bud6. Eukaryot Cell 5:881–895
33. Xu JR, Hamer JE (1996) MAP kinase and cAMP signaling regulate infection structure formation and pathogenic growth in the rice blast fungus Magnaporthe grisea. Genes Dev 10:2696–2706
34. Yang J, Zhao X, Sun J, Kang Z, Ding S, Xu JR, Peng YL (2010) A novel protein Com1 is required for normal conidium morphology and full virulence in Magnaporthe oryzae. Mol Plant Microbe Interact 23:112–123
35. Zheng XD, Wang YM, Wang Y (2003) CaSPA2 is important for polarity establishment and maintenance in Candida albicans. Mol Microbiol 49:1391–1405