Genome-wide functional analysis of pathogenicity genes in the rice blast fungus

Nature Genetics

Volumn 39, Issue 4, 2007, Pages 561-565

  Jeon, Junhyun ^a   Park, Sook Young ^a,c   Chi, Myoung Hwan ^a   Choi, Jaehyuk ^a   Park, Jongsun ^a   Rho, Hee Sool ^a   Kim, Soonok ^a   Goh, Jaeduk ^a   Yoo, Sungyong ^a   Choi, Jinhee ^a   Park, Ju Young ^a   Yi, Mihwa ^a   Yang, Seonyoung ^a   Kwon, Min Jung ^a   Han, Seong Sook ^b   Kim, Byeong Ryun ^b   Khang, Chang Hyun ^c   Park, Bongsoo ^a,c   Lim, Se Eun ^a   Jung, Kyongyong ^a   Kong, Sunghyung ^a   Karunakaran, Maruthachalam ^a   Oh, Hong Sik ^a   Kim, Hyojeong ^a   Kim, Seryun ^a   Park, Jaejin ^a   Kang, Soyoung ^a   Choi, Woo Bong ^d   Kang, Seogchan ^c   Lee, Yong Hwan ^a   more..

^a Seoul National University   (South Korea)

^b National Institute of Animal Science   (South Korea)

^c PENNSYLVANIA STATE UNIVERSITY   (United States)

^d Dong Eui University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; COMPARATIVE STUDY; FUNCTIONAL GENOMICS; FUNGAL LIFE CYCLE STAGE; FUNGAL VIRULENCE; FUNGUS; GENE LOCUS; GENE MUTATION; GENETIC SCREENING; GENETIC TRANSFECTION; GENOTYPE; HIGH THROUGHPUT SCREENING; INFORMATION SCIENCE; MAGNAPORTHE ORYZAE; MOLECULAR GENETICS; MOLECULAR MODEL; MUTAGENESIS; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PHENOTYPE; PRIORITY JOURNAL; RHIZOBIUM RADIOBACTER; RICE BLAST;

CHROMOSOME MAPPING; CHROMOSOMES, FUNGAL; GENES, FUNGAL; GENOME, FUNGAL; GENOTYPE; MAGNAPORTHE; MODELS, BIOLOGICAL; ORGANISMS, GENETICALLY MODIFIED; PHENOTYPE; RHIZOBIUM RADIOBACTER; VIRULENCE FACTORS;

AGROBACTERIUM TUMEFACIENS; FUNGI; MAGNAPORTHE GRISEA; MAGNAPORTHE ORYZAE;

EID: 34047150746     PISSN: 10614036     EISSN: 15461718     Source Type: Journal    
DOI: 10.1038/ng2002     Document Type: Article

References (30)

1. Galagan, J.E., Henn, M.R., Ma, L.J., Cuomo, C.A. & Birren, B. Genomics of the fungal kingdom: insights into eukaryotic biology. Genome Res. 15, 1620-1631 (2005).
2. Couch, B.C. & Kohn, L.M. A multilocus gene genealogy concordant with host preference indicates segregation of new species, Magnaporthe oryzae from M. grisea. Mycologia 94, 683-693 (2002).
3. Rho, H.S., Kang, S. & Lee, Y.H. Agrobacterium tumefaciens- mediated transformation of the plant pathogenic fungus, Magnaporthe grisea. Mol. Cells 12, 407-411 (2001).
4. Giaever, G. et al. Functional profiling of the Saccharomyces cerevisiae genome. Nature 418, 387-391 (2002).
5. Winzeler, E.A. et al. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285, 901-906 (1999).
6. Bevan, M. & Walsh, S. The Arabidopsis genome: a foundation for plant research. Genome Res. 15, 1632-1642 (2005).
7. Hirochika, H. et al. Rice mutant resources for gene discovery. Plant Mol. Biol. 54, 325-334 (2004).
8. Andres, A.J. Flying through the genome: a comprehensive study of functional genomics using RNAi in Drosophila. Trends Endocrinol. Metab. 15, 243-247 (2004).
9. Ashrafi, K. et al. Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes. Nature 421, 268-272 (2003).
10. Berns, K. et al. A large-scale RNAi screen in human cells identifies new components of the p53 pathway. Nature 428, 431-437 (2004).
11. Talbot, N.J. On the trail of a cereal killer: Exploring the biology of Magnaporthe grisea. Annu. Rev. Microbiol. 57, 177-202 (2003).
12. Kwon-Chung, K.J. & Bennett, J.E. Medical Mycology (Lea and Febiger, Philadelphia, 1992).
13. Dean, R.A. et al. The genome sequence of the rice blast fungus Magnaporthe grisea. Nature 434, 980-986 (2005).
14. Yu, J. et al. A draft sequence of the rice genome (Oryza sativa L. ssp. indica). Science 296, 79-92 (2002).
15. Goff, S.A. et al. A draft sequence of the rice genome (Oryza sativa L. ssp. japonica). Science 296, 92-100 (2002).
16. Krysan, P.J., Young, J.C. & Sussman, M.R. T-DNA as an insertional mutagen in Arabidopsis. Plant Cell 11, 2283-2290 (1999).
17. Idnurm, A. & Howlett, B.J. Pathogenicity genes of phytopathogenic fungi. Mol. Plant Pathol. 2, 241-255 (2001).
18. Liu, Y.G. & Whittier, R.F. Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25, 674-681 (1995).
19. Foster, A.J., Jenkinson, J.M. & Talbot, N.J. Trehalose synthesis and metabolism are required at different stages of plant infection by Magnaporthe grisea. EMBO J. 22, 225-235 (2003).
20. Xu, J.R., Staiger, C.J. & Hamer, J.E. Inactivation of the mitogen-activated protein kinase Mps1 from the rice blast fungus prevents penetration of host cells but allows activation of plant defense responses. Proc. Natl. Acad. Sci. USA 95, 12713-12718 (1998).
21. Bhambra, G.K., Wang, Z.Y., Soanes, D.M., Wakley, G.E. & Talbot, N.J. Peroxisomal carnitine acetyl transferase is required for elaboration of penetration hyphae during plant infection by Magnaporthe grisea. Mol. Microbiol. 61, 46-60 (2006).
22. Chen, C. & Dickman, M.B. Dominant active Rac and dominant negative Rac revert the dominant active Ras phenotype in Colletotrichum trifolii by distinct signalling pathways. Mol. Microbiol. 51, 1493-1507 (2004).
23. Semenza, J.C., Hardwick, K.G., Dean, N. & Pelham, H.R. ERD2, a yeast gene required for the receptor-mediated retrieval of luminal ER proteins from the secretory pathway. Cell 61, 1349-1357 (1990).
24. Tani, S. et al. Characterization of the amyR gene encoding a transcriptional activator for the amylase genes in Aspergillus nidulans. Curr. Genet. 39, 10-15 (2001).
25. Lee, K.S. & Levin, D.E. Dominant mutations in a gene encoding a putative protein kinase (BCK1) bypass the requirement for a Saccharomyces cerevisiae protein kinase C homolog. Mol. Cell. Biol. 12, 172-182 (1992).
26. Donofrio, N. et al. 'PACLIMS': a component LIM system for high-throughput functional genomic analysis. BMC Bioinformatics 10.1186/1471-2105-6-94 (2005).
27. Baker, E.J., Galloway, L., Jackson, B., Schmoyer, D. & Snoddy, J. MuTrack: a genome analysis system for large-scale mutagenesis in the mouse. BMC Bioinformatics 10.1186/1471-2105-5-11 (2004).
28. Mullins, E.D. & Kang, S. Transformation: a tool for studying fungal pathogens of plants. Cell. Mol. Life Sci. 58, 2043-2052 (2001).
29. Rogers, S.O. & Bendich, A.J. Extraction of DNA from milligram amount of fresh, herbarium, and mummified plant tissue. Plant Mol. Biol. 5, 69-76 (1985).
30. Yu, J.H. et al. Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi. Fungal Genet. Biol. 41, 973-981 (2004).