GC–MS based targeted metabolic profiling identifies changes in the wheat metabolome following deoxynivalenol treatment

Metabolomics

Volumn 11, Issue 3, 2015, Pages 722-738

  Warth, Benedikt ^a   Parich, Alexandra ^a   Bueschl, Christoph ^a   Schoefbeck, Denise ^a   Neumann, Nora Katharina Nicole ^a   Kluger, Bernhard ^a   Schuster, Katharina ^a   Krska, Rudolf ^a   Adam, Gerhard ^b   Lemmens, Marc ^a   Schuhmacher, Rainer ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Deoxynivalenol (vomitoxin); Fusarium head blight (scab); Metabolism; Metabolomics; Phenylpropanoids; Plant pathogen interaction; Wheat (Triticum aestivum)

Indexed keywords

EID: 84939888096     PISSN: 15733882     EISSN: 15733890     Source Type: Journal    
DOI: 10.1007/s11306-014-0731-1     Document Type: Article

References (57)

1. Abbas, H. K., Yoshizawa, T., & Shier, W. T. (2013). Cytotoxicity and phytotoxicity of trichothecene mycotoxins produced by Fusarium spp. Toxicon,74, 68–75.
2. Abolmaali, S., Mitterbauer, R., Spadiut, O., Peruci, M., Weindorfer, H., Lucyshyn, D., et al. (2008). Engineered bakers yeast as a sensitive bioassay indicator organism for the trichothecene toxin deoxynivalenol. Journal of Microbiological Methods,72(3), 306–312.
3. Abu Dawud, R., Schreiber, K., Schomburg, D., & Adjaye, J. (2012). Human embryonic stem cells and embryonal carcinoma cells have overlapping and distinct metabolic signatures. PLoS One,7(6), e39896.
4. Allwood, J. W., De Vos, R. C. H., Moing, A., Deborde, C., Erban, A., Kopka, J., et al. (2011). Plant metabolomics and its potential for systems biology research: Background concepts, technology, and methodology. Methods in Enzymology,500, 299–336.
5. Allwood, J. W., Erban, A., de Koning, S., Dunn, W. B., Luedemann, A., Lommen, A., et al. (2009). Inter-laboratory reproducibility of fast gas chromatography-electron impact-time of flight mass spectrometry (GC-EI-TOF/MS) based plant metabolomics. Metabolomics,5(4), 479–496.
6. Altpeter, F., & Posselt, U. K. (1994). Production of high quantities of 3-acetyldeoxynivalenol and deoxynivalenol. Applied Microbiology and Biotechnology,41(4), 384–387.
7. Anderson, J. A., Stack, R. W., Liu, S., Waldron, B. L., Fjeld, A. D., Coyne, C., et al. (2001). DNA markers for Fusarium head blight resistance QTLs in two wheat populations. Theoretical and Applied Genetics,102(8), 1164–1168.
8. Bai, G., Guo, P., & Kolb, F. L. (2003). Genetic relationships among head blight resistant cultivars of wheat assessed on the basis of molecular markers. Crop Science,43(2), 498–507.
9. Bassard, J.-E., Ullmann, P., Bernier, F., & Werck-Reichhart, D. (2010). Phenolamides: Bridging polyamines to the phenolic metabolism. Phytochemistry,71(16), 1808–1824.
10. Bollina, V., Kumaraswamy, G., Kushalappa, A. C., Choo, T., Dion, Y., Rioux, S., et al. (2010). Mass spectrometry-based metabolomics application to identify quantitative resistance-related metabolites in barley against Fusarium head blight. Molecular Plant Pathology,11(6), 769–782.
11. Bollina, V., Kushalappa, A. C., Choo, T. M., Dion, Y., & Rioux, S. (2011). Identification of metabolites related to mechanisms of resistance in barley against Fusarium graminearum, based on mass spectrometry. Plant Molecular Biology,77(4–5), 355–370.
12. Bolton, M. D. (2009). Current review: Primary metabolism and plant defense-fuel for the fire. Molecular Plant-Microbe Interactions,22(5), 487–497.
13. Buerstmayr, H., Ban, T., & Anderson, J. A. (2009). QTL mapping and marker-assisted selection for Fusarium head blight resistance in wheat: A review. Plant Breeding,128(1), 1–26.
14. Buerstmayr, H., Lemmens, M., Grausgruber, H., & Ruckenbauer, P. (1996). Scab resistance of international wheat germplasm. Cereal Research Communications,24(2), 195–202.
15. Bueschl, C., Kluger, B., Lemmens, M., Adam, G., Wiesenberger, G., Maschietto, V., et al. (2014). A novel stable isotope labelling assisted workflow for improved untargeted LC–HRMS based metabolomics research. Metabolomics, 10(4), 754–769.
16. Bushnell, W. R., Perkins-Veazie, P., Russo, V. M., Collins, J., & Seeland, T. M. (2009). Effects of deoxynivalenol on content of chloroplast pigments in barley leaf tissues. Phytopathology,100(1), 33–41.
17. Chakraborty, S., & Newton, A. C. (2011). Climate change, plant diseases and food security: an overview. Plant Pathology,60(1), 2–14.
18. Chen, L.-Q. (2014). SWEET sugar transporters for phloem transport and pathogen nutrition. New Phytologist,201(4), 1150–1155.
19. De Vos, R. C. H., Moco, S., Lommen, A., Keurentjes, J. J. B., Bino, R. J., & Hall, R. D. (2007). Untargeted large-scale plant metabolomics using liquid chromatography coupled to mass spectrometry. Nature Protocols,2(4), 778–791.
20. Desmond, O. J., Manners, J. M., Stephens, A. E., Maclean, D. J., Schenk, P. M., Gardiner, D. M., et al. (2008). The Fusarium mycotoxin deoxynivalenol elicits hydrogen peroxide production, programmed cell death and defence responses in wheat. Molecular Plant Pathology,9(4), 435–445.
21. Fiehn, O., Kopka, J., Dörmann, P., Altmann, T., Trethewey, R. N., & Willmitzer, L. (2000). Metabolite profiling for plant functional genomics. Nature Biotechnology,18(11), 1157–1161.
22. Fiehn, O., Sumner, L. W., Rhee, S. Y., Ward, J., Dickerson, J., Lange, B. M., et al. (2007). Minimum reporting standards for plant biology context information in metabolomic studies. Metabolomics,3(3), 195–201.
23. Gardiner, S. A., Boddu, J., Berthiller, F., Hametner, C., Stupar, R. M., Adam, G., et al. (2010a). Transcriptome analysis of the barley-deoxynivalenol interaction: evidence for a role of glutathione in deoxynivalenol detoxification. Molecular Plant-Microbe Interactions,23(7), 962–976.
24. Gardiner, D. M., Kazan, K., & Manners, J. (2009). Nutrient profiling reveals potent inducers of trichothecene biosynthesis in Fusarium graminearum. Fungal Genetics and Biology,46(8), 604–613.
25. Gardiner, D. M., Kazan, K., Praud, S., Torney, F. J., Rusu, A., & Manners, J. M. (2010b). Early activation of wheat polyamine biosynthesis during Fusarium head blight implicates putrescine as an inducer of trichothecene mycotoxin production. BMC Plant Biology,10, 289.
26. Goodacre, R., Broadhurst, D., Smilde, A. K., Kristal, B. S., Baker, J. D., Beger, R., et al. (2007). Proposed minimum reporting standards for data analysis in metabolomics. Metabolomics,3(3), 231–241.
27. Gunnaiah, R., Kushalappa, A. C., Duggavathi, R., Fox, S., & Somers, D. J. (2012). Integrated metabolo-proteomic approach to decipher the mechanisms by which wheat qtl (Fhb1) contributes to resistance against Fusarium graminearum. PLoS One,7(7), e40695.
28. Hamzehzarghani, H., Paranidharan, V., Abu-Nada, Y., Kushalappa, A. C., Mamer, O., & Somers, D. (2008). Metabolic profiling to discriminate wheat near isogenic lines, with quantitative trait loci at chromosome 2DL, varying in resistance to fusarium head blight. Canadian Journal of Plant Science,88(4), 789–797.
29. Hill, C. B., & Roessner, U. (2013). Metabolic profiling of plants by GC–MS. In W. Weckwerth & G. Kahl (Eds.), The handbook of plant metabolomics (pp. 1–23). Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA.
30. Hiller, K., Hangebrauk, J., Jäger, C., Spura, J., Schreiber, K., & Schomburg, D. (2009). Metabolite detector: Comprehensive analysis tool for targeted and nontargeted GC/MS based metabolome analysis. Analytical Chemistry,81(9), 3429–3439.
31. Hrydziuszko, O., & Viant, M. R. (2012). Missing values in mass spectrometry based metabolomics: An undervalued step in the data processing pipeline. Metabolomics,8, 161–174.
32. Kluger, B., Bueschl, C., Lemmens, M., Berthiller, F., Häubl, G., Jaunecker, G., et al. (2013). Stable isotopic labelling-assisted untargeted metabolic profiling reveals novel conjugates of the mycotoxin deoxynivalenol in wheat. Analytical and Bioanalytical Chemistry,405(15), 5031–5036.
33. Kugler, K. G., Siegwart, G., Nussbaumer, T., Ametz, C., Spannagl, M., Steiner, B., et al. (2013). Quantitative trait loci-dependent analysis of a gene co-expression network associated with Fusarium head blight resistance in bread wheat (Triticum aestivum L.). BMC Genomics,14(1), 728.
34. Kushalappa, A. C., & Gunnaiah, R. (2013). Metabolo-proteomics to discover plant biotic stress resistance genes. Trends in Plant Science, 18(9), 522–531.
35. Lehoczki-Krsjak, S., Szabó-Hevér, Á., Tóth, B., Kótai, C., Bartók, T., Varga, M., et al. (2010). Prevention of Fusarium mycotoxin contamination by breeding and fungicide application to wheat. Food Additives & Contaminants: Part A,27(5), 616–628.
36. Lemmens, M., Scholz, U., Berthiller, F., Dall’Asta, C., Koutnik, A., Schuhmacher, R., et al. (2005). The ability to detoxify the mycotoxin deoxynivalenol colocalizes with a major quantitative trait locus for fusarium head blight resistance in wheat. Molecular Plant-Microbe Interactions,18(12), 1318–1324.
37. Lisec, J., Schauer, N., Kopka, J., Willmitzer, L., & Fernie, A. R. (2006). Gas chromatography mass spectrometry-based metabolite profiling in plants. Nature Protocols,1(1), 387–396.
38. McMullen, M., Bergstrom, G., De Wolf, E., Dill-Macky, R., Hershman, D., Shaner, G., et al. (2012). A unified effort to fight an enemy of wheat and barley: Fusarium head blight. Plant Disease,96(12), 1712–1728.
39. Paulose, B., Chhikara, S., Coomey, J., Jung, H.-I., Vatamaniuk, O., & Dhankher, O. P. (2013). A γ-Glutamyl Cyclotransferase Protects Arabidopsis Plants from Heavy Metal Toxicity by Recycling Glutamate to Maintain Glutathione Homeostasis. The Plant Cell Online,25(11), 4580–4595.
40. Pestka, J. (2010). Deoxynivalenol: mechanisms of action, human exposure, and toxicological relevance. Archives of Toxicology,84(9), 663–679.
41. Poppenberger, B., Berthiller, F., Lucyshyn, D., Sieberer, T., Schuhmacher, R., Krska, R., et al. (2003). Detoxification of the Fusarium Mycotoxin Deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana. Journal of Biological Chemistry,278(48), 47905–47914.
42. Roessner, U., & Dias, D. A. (2013). Plant tissue extraction for metabolomics. Methods in Molecular Biology,1055, 21–28.
43. Schweiger, W., Steiner, B., Ametz, C., Siegwart, G., Wiesenberger, G., Berthiller, F., et al. (2013). Transcriptomic characterization of two major Fusarium resistance quantitative trait loci (QTLs), Fhb1 and Qfhs.ifa-5A, identifies novel candidate genes. Molecular Plant Pathology,14(8), 772–785.
44. Seifi, H. S., Van Bockhaven, J., Angenon, G., & Höfte, M. (2013). Glutamate metabolism in plant disease and defense: Friend or foe? Molecular Plant-Microbe Interactions,26(5), 475–485.
45. Šidák, Z. (1967). Rectangular Confidence Regions for the Means of Multivariate Normal Distributions. Journal of the American Statistical Association,62(318), 626–633.
46. Somers, D. J., Fedak, G., & Savard, M. (2003). Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat. Genome,46(4), 555–564.
47. Steuer, R., Morgenthal, K., Weckwerth, W., & Selbig, J. (2007). A gentle guide to the analysis of metabolomic data. Methods in Molecular Biology,358, 105–126.
48. Sumner, L. W., Amberg, A., Barrett, D., Beale, M. H., Beger, R., Daykin, C. A., et al. (2007). Proposed minimum reporting standards for chemical analysis: Chemical Analysis Working Group (CAWG) Metabolomics Standards Initiative (MSI). Metabolomics,3(3), 211–221.
49. R Development, & Team, C. (2012). R: A Language and Environment for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing, Retrieved from http://www.R-project.org.
50. Van de Poel, B., Bulens, I., Markoula, A., Hertog, M. L. A. T. M., Dreesen, R., Wirtz, M., et al. (2012). Targeted systems biology profiling of tomato fruit reveals coordination of the yang cycle and a distinct regulation of ethylene biosynthesis during postclimacteric ripening. Plant Physiology,160(3), 1498–1514.
51. Van den Berg, R., Hoefsloot, H., Westerhuis, J., Smilde, A., & van der Werf, M. (2006). Centering, scaling, and transformations: improving the biological information content of metabolomics data. BMC Genomics,7(1), 142.
52. Vincent, D., Du Fall, L. A., Livk, A., Mathesius, U., Lipscombe, R. J., Oliver, R. P., et al. (2011). A functional genomics approach to dissect the mode of action of the Stagonospora nodorum effector protein SnToxA in wheat. Molecular Plant Pathology,13(5), 467–482.
53. Walter, S., Brennan, J., Arunachalam, C., Ansari, K., Hu, X., Khan, M., et al. (2008). Components of the gene network associated with genotype-dependent response of wheat to the Fusarium mycotoxin deoxynivalenol. Functional & Integrative Genomics,8(4), 421–427.
54. Walter, S., & Doohan, F. (2011). Transcript profiling of the phytotoxic response of wheat to the Fusarium mycotoxin deoxynivalenol. Mycotoxin Research,27, 221–230.
55. Walter, S., Nicholson, P., & Doohan, F. M. (2010). Action and reaction of host and pathogen during Fusarium head blight disease. New Phytologist,185(1), 54–66.
56. Wehrens, R. (2011). Chemometrics with R: Multivariate data analysis in the natural sciences and life sciences. Heidelberg: Springer.
57. Witte, C.-P. (2011). Urea metabolism in plants. Plant Science,180(3), 431–438.