Capsid-Damaging Effects of UV Irradiation as Measured by Quantitative PCR Coupled with Ethidium Monoazide Treatment

Food and Environmental Virology

Volumn 6, Issue 4, 2014, Pages 269-275

  Sangsanont, J ^a   Katayama, H ^a   Kurisu, F ^a   Furumai, H ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Ethidium monoazide; UV irradiation; Viral capsid damage; Viral genome damage

Indexed keywords

8-AZIDOETHIDIUM; AFFINITY LABELING; AZIDE; VIRUS DNA; VIRUS RNA;

AFFINITY LABELING; ANIMAL; CELL LINE; CHLOROCEBUS AETHIOPS; COMPARATIVE STUDY; DISINFECTION; EVALUATION STUDY; GROWTH, DEVELOPMENT AND AGING; HUMAN; HUMAN ADENOVIRUS C; METABOLISM; PATHOGENICITY; PHARMACOLOGY; POLIOMYELITIS VIRUS; PRESSURE; PROCEDURES; RADIATION RESPONSE; RADIATION TOLERANCE; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ULTRAVIOLET RADIATION; VIRAL PLAQUE ASSAY; VIRUS CAPSID; VIRUS GENOME; VIRUS INACTIVATION;

ADENOVIRUSES, HUMAN; AFFINITY LABELS; ANIMALS; AZIDES; CAPSID; CELL LINE; CERCOPITHECUS AETHIOPS; DISINFECTION; DNA, VIRAL; GENOME, VIRAL; HUMANS; POLIOVIRUS; PRESSURE; RADIATION TOLERANCE; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; ULTRAVIOLET RAYS; VIRAL PLAQUE ASSAY; VIRUS INACTIVATION;

EID: 84939883924     PISSN: 18670334     EISSN: 18670342     Source Type: Journal    
DOI: 10.1007/s12560-014-9162-4     Document Type: Article

References (19)

1. Currell, G., & Dowman, A. A. (2009). Essential mathematics and statistics for science (2nd ed.). Hoboken, NJ: Wiley–Blackwell.
2. Eischeid, A. C., & Linden, K. G. (2011). Molecular indications of protein damage in adenoviruses after UV disinfection. Applied and Environmental Microbiology,77, 1145–1147.
3. Fittipaldi, M., Rodriguez, N. J., Codony, B., Adrados, B., Penuela, G. A., & Morato, J. (2010). Discrimination of infectious bacteriophage T4 virus by propidiummonoazide real-time PCR. Journal Virology Methods,168, 228–232.
4. Guo, H., Chu, X., & Hu, J. (2010). Effect of host cells on low- and medium-pressure UV inactivation of adenoviruses. Applied and Environmental Microbiology,76, 7068–7075.
5. Harm, W. (1980). Biological effects of ultraviolet radiation. Cambridge: Cambridge University Press.
6. Heim, A., Ebnet, C., Harste, G., & Pring-Akerblom, P. (2003). Rapid and quantitative detection of human adenovirus DNA by real time PCR. Journal of Medical Virology,70, 228–239.
7. Hijnen, W. A., Beerendonk, E. F., & Medema, G. J. (2006). Inactivation credit of UV radiation for viruses, bacteria and protozoan (oo)cysts in water: A review. Water Research,40, 3–22.
8. Jin, S., Mofidi, A. A., & Linden, K. G. (2006). Polychromatic UV fluence measurement using chemical actinometry, biodosimetry, and mathematical techniques. Journal of Environmental Engineering,132, 831–841.
9. Kamiko, N., & Ohgaki, S. (1989). RNA coliphage Qβ as a bioindicator of the UV Disinfection efficiency. Water Science and Technology,21(3), 227–231.
10. Katayama, H., Shimasaki, A., & Ohgaki, S. (2002). Development of a virus concentration method and its application to detection of enterovirus and Norwalk virus from coastal seawater. Applied and Environmental Microbiology,68, 1033–1039.
11. Kim, K., Katayama, H., Kitajima, M., Tohya, Y., & Ohgaki, S. (2011). Development of a real-time RT-PCR assay combined with ethidiummonoazide treatment for RNA viruses and its application to detect viral RNA after heat exposure. Water Science and Technology,63(3), 502–507.
12. Ko, G., Cromeans, T. L., & Sobsey, M. D. (2005). UV inactivation of adenovirus type 41 measured by cell culture mRNA RT-PCR. Water Research,39, 3643–3649.
13. Linden, K. G., Thurston, J., Schaefer, R., & Malley, J. P. (2007). Enhanced UV inactivation of Adenoviruses under polychromatic UV lamps. Applied and Environmental Microbiology,73(23), 7571–7574.
14. Masschelein, W. J. (2002). Ultraviolet light in water and wastewater sanitation. Edited for English by Rip G. Rice. Boca Raton, FL: Lewis Publishers.
15. Nwachcuku, N., & Gerba, C. P. (2004). Emerging waterborne pathogens: Can we kill them all? Current Opinion in Biotechnology,15, 175–180.
16. Parshionikar, S., Laseke, I., & Fout, G. S. (2010). Use of propidiummonoazide in reverse transcriptase PCR to distinguish between infectious and noninfectious enteric viruses in water samples. Applied and Environmental Microbiology,76, 4318–4326.
17. Porterfield, J. Z., & Zlotnick, A. (2010). A simple and general method for determining the protein and nucleic acid content of viruses by UV absorbance. Virology,407, 281–288.
18. Rahn, R. O. (1993). Use of potassium iodide as a chemical actinometer. Photochemistry and Photobiology,58, 874–880.
19. Yates, M., Malley, J., Rochelle, P., & Hoffman, R. (2006). Effect of adenovirus resistance on UV disinfection requirements: Report on the state of adenovirus science. American Water Works Association Journal,98, 93–106.