Characterization of Mechanical and Regenerative Properties of Human, Adipose Stromal Cells

Cellular and Molecular Bioengineering

Volumn 7, Issue 4, 2014, Pages 585-597

  Kanthilal, Manisha ^a,b   Darling, Eric M ^a,b,c  

^a BROWN UNIVERSITY   (United States)

^b BROWN UNIVERSITY   (United States)

^c Brown University   (United States)

Author keywords

Adipose derived stem cell; Atomic force microscope; Cell mechanics; Differentiation; Human lipoaspirate; Mechanical biomarkers; Stromal vascular fraction

Indexed keywords

EID: 84939877986     PISSN: 18655025     EISSN: 18655033     Source Type: Journal    
DOI: 10.1007/s12195-014-0350-y     Document Type: Article

References (41)

1. Baer, P. C. Adipose-derived stem cells and their potential to differentiate into the epithelial lineage. Stem Cells and Development 20(10):1805–1816, 2011.
2. Baer, P. C., and H. Geiger. Adipose-derived mesenchymal stromal/stem cells: Tissue localization, characterization, and heterogeneity. Stem Cells International 2012:812693, 2012.
3. Bianco, P., X. Cao, P. S. Frenette, J. J. Mao, P. G. Robey, P. J. Simmons, and C. Y. Wang. The meaning, the sense and the significance: Translating the science of mesenchymal stem cells into medicine. Nature Medicine 19(1):35–42, 2013.
4. Bourin, P., B. A. Bunnell, L. Casteilla, M. Dominici, A. J. Katz, K. L. March, H. Redl, J. P. Rubin, K. Yoshimura, and J. M. Gimble. Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: A joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT). Cytotherapy 15(6):641–648, 2013.
5. Carpenter, A. E., T. R. Jones, M. R. Lamprecht, C. Clarke, I. H. Kang, O. Friman, D. A. Guertin, J. H. Chang, R. A. Lindquist, J. Moffat, P. Golland, and D. M. Sabatini. Cell Profiler: Image analysis software for identifying and quantifying cell phenotypes. Genome Biology 7(10):R100, 2006.
6. Darling, E. M., M. Topel, S. Zauscher, T. P. Vail, and F. Guilak. Viscoelastic properties of human mesenchymally-derived stem cells and primary osteoblasts, chondrocytes, and adipocytes. Journal of Biomechanics 41(2):454–464, 2008.
7. Darling, E. M., S. Zauscher, J. A. Block, and F. Guilak. A thin-layer model for viscoelastic, stress-relaxation testing of cells using atomic force microscopy: Do cell properties reflect metastatic potential? Biophysical Journal 92(5):1784–1791, 2007.
8. Darling, E. M., S. Zauscher, and F. Guilak. Viscoelastic properties of zonal articular chondrocytes measured by atomic force microscopy. Osteoarthritis Cartilage 14(6):571–579, 2006.
9. De Francesco, F., V. Tirino, V. Desiderio, G. Ferraro, F. D’Andrea, M. Giuliano, G. Libondi, G. Pirozzi, A. De Rosa, and G. Papaccio. Human CD34(+)/CD90(+) ASCs are capable of growing as sphere clusters, producing high levels of VEGF and forming capillaries. Plos One 4(8):e6537, 2009.
10. Dimitriadis, E. K., F. Horkay, J. Maresca, B. Kachar, and R. S. Chadwick. Determination of elastic moduli of thin layers of soft material using the atomic force microscope. Biophysical Journal 82(5):2798–2810, 2002.
11. Ekpenyong, A. E., G. Whyte, K. Chalut, S. Pagliara, F. Lautenschlager, C. Fiddler, S. Paschke, U. F. Keyser, E. R. Chilvers, and J. Guck. Viscoelastic properties of differentiating blood cells are fate- and function-dependent. Plos One 7(9):e45237, 2012.
12. Estes, B. T., B. O. Diekman, J. M. Gimble, and F. Guilak. Isolation of adipose-derived stem cells and their induction to a chondrogenic phenotype. Nature Protocols 5(7):1294–1311, 2010.
13. Festy, F., L. Hoareau, S. Bes-Houtmann, A. M. Pequin, M. P. Gonthier, A. Munstun, J. J. Hoarau, M. Cesari, and R. Roche. Surface protein expression between human adipose tissue-derived stromal cells and mature adipocytes. Histochemistry and Cell Biology 124(2):113–121, 2005.
14. Gimble, J. M., A. J. Katz, and B. A. Bunnell. Adipose-derived stem cells for regenerative medicine. Circulation Research 100(9):1249–1260, 2007.
15. Gonzalez-Cruz, R. D., V. C. Fonseca, and E. M. Darling. Cellular mechanical properties reflect the differentiation potential of adipose-derived mesenchymal stem cells. Proc Natl Acad Sci USA 109(24):E1523–E1529, 2012.
16. Gossett, D. R., W. M. Weaver, A. J. Mach, S. C. Hur, H. T. Tse, W. Lee, H. Amini, and D. Di Carlo. Label-free cell separation and sorting in microfluidic systems. Analytical and Bioanalytical Chemistry 397(8):3249–3267, 2010.
17. Gronthos, S., D. M. Franklin, H. A. Leddy, P. G. Robey, R. W. Storms, and J. M. Gimble. Surface protein characterization of human adipose tissue-derived stromal cells. Journal of Cellular Physiology 189(1):54–63, 2001.
18. Guilak, F., K. E. Lott, H. A. Awad, Q. F. Cao, K. C. Hicok, B. Fermor, and J. M. Gimble. Clonal analysis of the differentiation potential of human adipose-derived adult stem cells. Journal of Cellular Physiology 206(1):229–237, 2006.
19. Hochmuth, R. M. Micropipette aspiration of living cells. Journal of Biomechanics 33(1):15–22, 2000.
20. Hur, S. C., N. K. Henderson-MacLennan, E. R. B. McCabe, and D. Di Carlo. Deformability-based cell classification and enrichment using inertial microfluidics. Lab on a Chip 11(5):912–920, 2011.
21. Hutter, J. L., and J. Bechhoefer. Calibration of atomic-force microscope tips. Review of Scientific Instruments 64(7):1868–1873, 1993.
22. Izadpanah, R., D. Kaushal, C. Kriedt, F. Tsien, B. Patel, J. Dufour, and B. A. Bunnell. Long-term in vitro expansion alters the biology of adult mesenchymal stem cells. Cancer Research 68(11):4229–4238, 2008.
23. Li, H., L. Zimmerlin, K. G. Marra, V. S. Donnenberg, A. D. Donnenberg, and J. P. Rubin. Adipogenic potential of adipose stem cell subpopulations. Plastic and Reconstructive Surgery 128(3):663–672, 2011.
24. Lin, K., Y. Matsubara, Y. Masuda, K. Togashi, T. Ohno, T. Tamura, Y. Toyoshima, K. Sugimachi, M. Toyoda, H. Marc, and A. Douglas. Characterization of adipose tissue-derived cells isolated with the Celution system. Cytotherapy 10(4):417–426, 2008.
25. Maloney, J. M., D. Nikova, F. Lautenschlager, E. Clarke, R. Langer, J. Guck, and K. J. Van Vliet. Mesenchymal stem cell mechanics from the attached to the suspended state. Biophysical Journal 99(8):2479–2487, 2010.
26. McIntosh, K., S. Zvonic, S. Garrett, J. B. Mitchell, Z. E. Floyd, L. Hammill, A. Kloster, Y. Di Halvorsen, J. P. Ting, R. W. Storms, B. Goh, G. Kilroy, X. Wu, and J. M. Gimble. The immunogenicity of human adipose-derived cells: Temporal changes in vitro. Stem Cells 24(5):1246–1253, 2006.
27. Mitchell, J. B., K. McIntosh, S. Zvonic, S. Garrett, Z. E. Floyd, A. Kloster, Y. Di Halvorsen, R. W. Storms, B. Goh, G. Kilroy, X. Wu, and J. M. Gimble. Immunophenotype of human adipose-derived cells: Temporal changes in stromal-associated and stem cell-associated markers. Stem Cells 24(2):376–385, 2006.
28. Nilsson, S. K., M. S. Dooner, C. Y. Tiarks, H. U. Weier, and P. J. Quesenberry. Potential and distribution of transplanted hematopoietic stem cells in a nonablated mouse model. Blood 89(11):4013–4020, 1997.
29. Reddy, G. P. V., C. Y. Tiarks, L. Z. Pang, J. Wuu, C. C. Hsieh, and P. J. Quesenberry. Cell cycle analysis and synchronization of pluripotent hematopoietic progenitor stem cells. Blood 90(6):2293–2299, 1997.
30. Relou, I. A. M., C. A. Damen, D. W. J. van der Schaft, G. Groenewegen, and A. W. Griffioen. Effect of culture conditions on endothelial cell growth and responsiveness. Tissue and Cell 30(5):525–530, 1998.
31. Sawetzki, T., C. D. Eggleton, S. A. Desai, and D. W. M. Marr. Viscoelasticity as a biomarker for high-throughput flow cytometry. Biophysical Journal 105(10):2281–2288, 2013.
32. Strem, B. M., K. C. Hicok, M. Zhu, I. Wulur, Z. Alfonso, R. E. Schreiber, J. K. Fraser, and M. H. Hedrick. Multipotential differentiation of adipose tissue-derived stem cells. Keio Journal of Medicine 54(3):132–141, 2005.
33. Strioga, M., S. Viswanathan, A. Darinskas, O. Slaby, and J. Michalek. Same or not the same? Comparison of adipose tissue-derived vs. bone marrow-derived mesenchymal stem and stromal cells. Stem Cells and Development 21(14):2724–2752, 2012.
34. Suga, H., D. Matsumoto, H. Eto, K. Inoue, N. Aoi, H. Kato, J. Araki, and K. Yoshimura. Functional implications of CD34 expression in human adipose-derived stem/progenitor cells. Stem Cells and Development 18(8):1201–1210, 2009.
35. Suresh, S. Biomechanics and biophysics of cancer cells. Acta Biomaterialia 3(4):413–438, 2007.
36. Teboul, L., M. Febbraio, D. Gaillard, E. Z. Amri, R. Silverstein, and P. A. Grimaldi. Structural and functional characterization of the mouse fatty acid translocase promoter: Activation during adipose differentiation. Biochemical Journal 360(Pt 2):305–312, 2001.
37. Varma, M. J., R. G. Breuls, T. E. Schouten, W. J. Jurgens, H. J. Bontkes, G. J. Schuurhuis, S. M. van Ham, and F. J. van Milligen. Phenotypical and functional characterization of freshly isolated adipose tissue-derived stem cells. Stem Cells and Development 16(1):91–104, 2007.
38. Yourek, G., M. A. Hussain, and J. J. Mao. Cytoskeletal changes of mesenchymal stem cells during differentiation. ASAIO Journal 53(2):219–228, 2007.
39. Zheng, B., B. H. Cao, G. H. Li, and J. Huard. Mouse adipose-derived stem cells undergo multilineage differentiation in vitro but primarily osteogenic and chondrogenic differentiation in vivo. Tissue Engineering 12(7):1891–1901, 2006.
40. Zhu, Y., T. Liu, K. Song, X. Fan, X. Ma, and Z. Cui. Adipose-derived stem cell: A better stem cell than BMSC. Cell Biochemistry and Function 26(6):664–675, 2008.
41. Zuk, P. A., M. Zhu, H. Mizuno, J. Huang, J. W. Futrell, A. J. Katz, P. Benhaim, H. P. Lorenz, and M. H. Hedrick. Multilineage cells from human adipose tissue: Implications for cell-based therapies. Tissue Engineering 7(2):211–228, 2001.