Human CD34+/CD90+ ASCs are capable of growing as sphere clusters, producing high levels of VEGF and forming capillaries

PLoS ONE

Volumn 4, Issue 8, 2009, Pages

  De Francesco, Francesco ^a   Tirino, Virginia ^a   Desiderio, Vincenzo ^a   Ferraro, Giuseppe ^a   D'Andrea, Francesco ^a   Giuliano, Mariateresa ^a   Libondi, Guido ^a   Pirozzi, Giuseppe ^b   De Rosa, Alfredo ^a   Papaccio, Gianpaolo ^a  

^a SECOND UNIVERSITY OF NAPLES   (Italy)

^b NATIONAL CANCER INSTITUTE   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CD31 ANTIGEN; CD34 ANTIGEN; CELL MARKER; HERMES ANTIGEN; INTERCELLULAR ADHESION MOLECULE 1; METHYLCELLULOSE; TELOMERASE; THY 1 ANTIGEN; VASCULOTROPIN; VASCULOTROPIN RECEPTOR 2; VASCULOTROPIN A;

ADIPOCYTE; ADIPOSE TISSUE CELL; ADULT; ARTICLE; CAPILLARY; CELL DIFFERENTIATION; CELL FRACTIONATION; CELL GROWTH; CELL POPULATION; CELL PROLIFERATION; CONTROLLED STUDY; CULTURE MEDIUM; ENDOTHELIUM CELL; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IN VITRO STUDY; PHENOTYPE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STEM CELL; ADIPOSE TISSUE; BIOSYNTHESIS; CELL DIVISION; CYTOLOGY; GROWTH, DEVELOPMENT AND AGING; IMMUNOLOGY; METABOLISM;

ADIPOSE TISSUE; ANTIGENS, CD34; ANTIGENS, THY-1; CAPILLARIES; CELL DIFFERENTIATION; CELL DIVISION; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; HUMANS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STEM CELLS; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

EID: 68449104766     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0006537     Document Type: Article

References (33)

1. Hombach-Klonisch S, Panigrahi S, Rashedi I, Seifert A, Alberti E, et al. (2008) Adult stem cells and their trans-differentiation potential-perspectives and therapeutic applications. J Mol Med 86: 1301-1314.
2. Verfaillie CM, Pera MF, Lansdorp PM (2002) Stem cells: hype and reality. Hematology Am Soc Hematol Educ Program. pp 369-391.
3. Conget PA, Minguell JJ (1999) Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells. J Cell Physiol 181: 67-73.
4. Majumdar MK, Thiede MA, Mosca JD, Moorman M, Gerson SL (1998) Phenotypic and functional comparison of cultures of marrow-derived mesenchymal stem cells (MSCs) and stromal cells. J Cell Physiol 176: 57-66.
5. Schilling T, Noth U, Klein-Hitpass L, Jakob F, Schutze N (2007) Plasticity in adipogenesis and osteogenesis of human mesenchymal stem cells. Mol Cell Endocrinol 271: 1-17.
6. Pittenger MF (2008) Mesenchymal stem cells from adult bone marrow. Methods Mol Biol 449: 27-44.
7. Ryden M, Dicker A, Gotherstrom C, Astrom G, Tammik C, et al. (2003) Functional characterization of human mesenchymal stem cell-derived adipocytes. Biochem Biophys Res Commun 311: 391-397.
8. d'Aquino R, Graziano A, Sampaolesi M, Laino G, Pirozzi G, et al. (2007) Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation. Cell Death Differ 14: 1162-1171.
9. Laino G, d'Aquino R, Graziano A, Lanza V, Carinci F, et al. (2005) A new population of human adult dental pulp stem cells: a useful source of living autologous fibrous bone tissue (LAB). J Bone Miner Res 20: 1394-1402.
10. Lee OK, Kuo TK, Chen WM, Lee KD, Hsieh SL, et al. (2004) Isolation of multipotent mesenchymal stem cells from umbilical cord blood. Blood 103: 1669-1675.
11. Romanov YA, Svintsitskaya VA, Smirnov VN (2003) Searching for alternative sources of postnatal human mesenchymal stem cells: candidate MSC-like cells from umbilical cord. Stem Cells 21: 105-110.
12. Niknejad H, Peirovi H, Jorjani M, Ahmadiani A, Ghanavi J, et al. (2008) Properties of the amniotic membrane for potential use in tissue engineering. Eur Cell Mater 15: 88-99.
13. Fraser JK, Wulur I, Alfonso Z, Hedrick MH (2006) Fat tissue: an underappreciated source of stem cells for biotechnology. Trends Biotechnol 24: 150-154.
14. Gronthos S, Franklin DM, Leddy HA, Robey PG, Storms RW, et al. (2001) Surface protein characterization of human adipose tissue-derived stromal cells. J Cell Physiol 189: 54-63.
15. Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, et al. (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13: 4279-4295.
16. De Ugarte DA, Morizono K, Elbarbary A, Alfonso Z, Zuk PA, et al. (2003) Comparison of multi-lineage cells from human adipose tissue and bone marrow. Cells Tissues Organs 174: 101-109.
17. Gimble J, Guilak F (2003) Adipose-derived adult stem cells: isolation, characterization, and differentiation potential. Cytotherapy 5: 362-369.
18. Planat-Benard V, Silvestre JS, Cousin B, Andre M, Nibbelink M, et al. (2004) Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives. Circulation 109: 656-663.
19. Erickson GR, Gimble JM, Franklin DM, Rice HE, Awad H, et al. (2002) Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo. Biochem Biophys Res Commun 290: 763-769.
20. Halvorsen YD, Franklin D, Bond AL, Hitt DC, Auchter C, et al. (2001) Extracellular matrix mineralization and osteoblast gene expression by human adipose tissue-derived stromal cells. Tissue Eng 7: 729-741.
21. Sengenes C, Miranville A, Maumus M, de Barros S, Busse R, et al. (2007) Chemotaxis and differentiation of human adipose tissue CD34+/ CD31-progenitor cells: role of stromal derived factor-1 released by adipose tissue capillary endothelial cells. Stem Cells 25: 2269-2276.
22. Miranville A, Heeschen C, Sengenes C, Curat CA, Busse R, et al. (2004) Improvement of postnatal neovascularization by human adipose tissue-derived stem cells. Circulation 110: 349-355.
23. Rehman J, Traktuev D, Li J, Merfeld-Clauss S, Temm-Grove CJ, et al. (2004) Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation 109: 1292-1298.
24. D'Andrea F, De Francesco F, Ferraro GA, Desiderio V, Tirino V, et al. (2008) Large-Scale Production of Human Adipose Tissue from Stem Cells: A New Tool for Regenerative Medicine and Tissue Banking. Tissue Eng Part A.
25. Greaves MF, Brown J, Molgaard HV, Spurr NK, Robertson D, et al. (1992) Molecular features of CD34: a hemopoietic progenitor cell-associated molecule. Leukemia 6 Suppl 1: 31-36.
26. Fina L, Molgaard HV, Robertson D, Bradley NJ, Monaghan P, et al. (1990) Expression of the CD34 gene in vascular endothelial cells. Blood 75: 2417-2426.
27. Rafii S, Lyden D (2003) Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration. Nat Med 9: 702-712.
28. Maruyama K, Mori Y, Murasawa S, Masaki H, Takahashi N, et al. (1999) Interleukin-1 beta upregulates cardiac expression of vascular endothelial growth factor and its receptor KDR/flk-1 via activation of protein tyrosine kinases. J Mol Cell Cardiol 31: 607-617.
29. Isner JM (1996) Therapeutic angiogenesis: a new frontier for vascular therapy. Vasc Med 1: 79-87.
30. Isner JM, Asahara T (1999) Angiogenesis and vasculogenesis as therapeutic strategies for postnatal neovascularization. J Clin Invest 103: 1231-1236.
31. Kalka C, Masuda H, Takahashi T, Kalka-Moll WM, Silver M, et al. (2000) Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization. Proc Natl Acad Sci U S A 97: 3422-3427.
32. Cao Y, Sun Z, Liao L, Meng Y, Han Q, et al. (2005) Human adipose tissue-derived stem cells differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo. Biochem Biophys Res Commun 332: 370-379.
33. De Rosa A, De Francesco F, Tirino V, Ferraro GA, Paino F, et al. (2009) A new method for the cryopreserving ASCs: an attractive and suitable large-scale and long-term cell banking technology. Tissue Eng Part C Methods.