A simple, straightforward correlative live-cell-imaging-structured-illumination-microscopy approach for studying organelle dynamics

Microscopy Research and Technique

Volumn 78, Issue 9, 2015, Pages 777-783

  Sherman, Shachar ^a   Nachmias, Dikla ^a   Elia, Natalie ^a  

^a BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

Author keywords

Fission; Fusion; Light microscopy; Membrane dynamics; Mitochondria; SIM; Spinning Disk

Indexed keywords

CELL MEMBRANES; CYTOLOGY; DYNAMICS;

CELLULAR ORGANELLES; FISSION; LIVE-CELL IMAGING; MEMBRANE DYNAMICS; MEMBRANE FISSION; MITOCHONDRIAS; SIMPLE++; SPINNING DISKS; STRUCTURED ILLUMINATION; STRUCTURED ILLUMINATION MICROSCOPY;

MITOCHONDRIA;

ANIMAL; CELL ORGANELLE; DOG; FLUORESCENCE IMAGING; FLUORESCENCE MICROSCOPY; MDCK CELL LINE; PHYSIOLOGY; PROCEDURES; ULTRASTRUCTURE; VIDEO MICROSCOPY;

ANIMALS; DOGS; MADIN DARBY CANINE KIDNEY CELLS; MICROSCOPY, FLUORESCENCE; MICROSCOPY, VIDEO; OPTICAL IMAGING; ORGANELLES;

EID: 84939650915     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.22540     Document Type: Article

References (15)

1. Elia N, Ott C, Lippincott-Schwartz J. 2013. Incisive imaging and computation for cellular mysteries: Lessons from abscission. Cell 155:1220-1231.
2. Fiolka R, Shao L, Rego EH, Davidson MW, Gustafsson MG. 2012. Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination. Proc Natl Acad Sci USA 109:5311-5315.
3. Forster R, Lu-Walther HW, Jost A, Kielhorn M, Wicker K, Heintzmann R. 2014. Simple structured illumination microscope setup with high acquisition speed by using a spatial light modulator. Opt Express 22:20663-20677.
4. Friedman JR, Nunnari J. 2014. Mitochondrial form and function. Nature 505:335-343.
5. Gustafsson MG. 2000. Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. J Microsc 198:82-87.
6. Hayashi S, Okada Y. 2015. Ultrafast superresolution fluorescence imaging with spinning disk confocal microscope optics. Mol Biol Cell 26(9):1743-1751.
7. Heintzmann R, Cremer CG. 1999. Laterally modulated excitation microscopy: Improvement of resolution by using a diffraction grating. Proc Spie 3568:185-196.
8. Hirvonen LM, Wicker K, Mandula O, Heintzmann R. 2009. Structured illumination microscopy of a living cell. Eur Biophys J EBJ 38:807-812.
9. Huang B, Babcock H, Zhuang X. 2010. Breaking the diffraction barrier: Super-resolution imaging of cells. Cell 143:1047-1058.
10. Hubner B, Cremer T, Neumann J. 2013. Correlative microscopy of individual cells: Sequential application of microscopic systems with increasing resolution to study the nuclear landscape. Methods Mol Biol 1042:299-336.
11. Jakobs S, Wurm CA. 2014. Super-resolution microscopy of mitochondria. Curr Opin Chem Biol 20:9-15.
12. Shao L, Kner P, Rego EH, Gustafsson MG. 2011. Super-resolution 3D microscopy of live whole cells using structured illumination. Nat Methods 8:1044-1046.
13. Tam J, Cordier GA, Balint S, Sandoval Alvarez A, Borbely JS, Lakadamyali M. 2014. A microfluidic platform for correlative live-cell and super-resolution microscopy. PloS One 9:e115512.
14. Turnbull L, Strauss MP, Liew AT, Monahan LG, Whitchurch CB, Harry EJ. 2014. Super-resolution imaging of the cytokinetic Z ring in live bacteria using fast 3D-structured illumination microscopy (f3D-SIM). J Vis Exp JoVE 91:e51469.
15. York AG, Parekh SH, Dalle Nogare D, Fischer RS, Temprine K, Mione M, Chitnis AB, Combs CA, Shroff H. 2012. Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy. Nat Methods 9:749-754.