High yield purification of plasmodium falciparum merozoites for use in opsonizing antibody assays

Journal of Visualized Experiments

Volumn , Issue 89, 2014, Pages

  Hill, Danika L ^a,b   Eriksson, Emily M ^a,b   Schofield, Louis ^a,b  

^a WALTER AND ELIZA HALL INSTITUTE OF MEDICAL RESEARCH   (Australia)

^b UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Antibody; Fc receptor; Hemozoin; Immunology; Issue 89; Malaria; Merozoite; Opsonization; Parasitic diseases; Phagocytosis; Plasmodium falciparum; THP 1

Indexed keywords

EID: 84939498772     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51590     Document Type: Article

References (35)

1. Cohen, S., McGregor, I. A., & Carrington, S. Gamma-globulin and acquired immunity to human malaria. Nature. 192, 733-737 (1961).
2. Fowkes, F. J. I., Richards, J. S., Simpson, J. A., & Beeson, J. G. The relationship between anti-merozoite antibodies and incidence of Plasmodium falciparum malaria: A systematic review and meta-analysis. PLoS Medicine. 7, e1000218, doi:10.1371/journal.pmed.1000218 (2010).
3. Bouharoun-Tayoun, H., Attanath, P., Sabchareon, A., Chongsuphajaisiddhi, T., & Druilhe, P. Antibodies that protect humans against Plasmodium falciparum blood stages do not on their own inhibit parasite growth and invasion in vitro, but act in cooperation with monocytes. The Journal of experimental medicine. 172, 1633-1641 (1990).
4. Jafarshad, A. et al. A novel antibody-dependent cellular cytotoxicity mechanism involved in defense against malaria requires costimulation of monocytes FcgammaRII and FcgammaRIII. Journal of immunology (Baltimore, Md: 1950). 178, 3099-3106 (2007).
5. Genton, B. et al. A recombinant blood-stage malaria vaccine reduces Plasmodium falciparum density and exerts selective pressure on parasite populations in a phase 1-2b trial in Papua New Guinea. The Journal of Infectious Diseases. 185, 820-827, doi:10.1086/339342 (2002).
6. Sirima, S. B., Cousens, S., & Druilhe, P. Protection against malaria by MSP3 candidate vaccine. The New England journal of medicine. 365, 1062-1064, doi:10.1056/NEJMc1100670 (2011).
7. Llewellyn, D. et al. Assessment of antibody-dependent respiratory burst activity from mouse neutrophils on Plasmodium yoelii malaria challenge outcome. Journal of leukocyte biology, doi:10.1189/jlb.0513274 (2013).
8. McIntosh, R. S. et al. The importance of human FcgammaRI in mediating protection to malaria. PLoS pathogens. 3, e72, doi:10.1371/journal.ppat.0030072 (2007).
9. Pleass, R. J. et al. Novel antimalarial antibodies highlight the importance of the antibody Fc region in mediating protection. Blood. 102, 4424-4430, doi:10.1182/blood-2003-02-0583 (2003).
10. Hill, D. L. et al. Opsonizing Antibodies to P. falciparum Merozoites Associated with Immunity to Clinical Malaria. PLoS One. 8, e74627, doi:10.1371/journal.pone.0074627 (2013).
11. Joos, C. et al. Clinical Protection from Falciparum Malaria Correlates with Neutrophil Respiratory Bursts Induced by Merozoites Opsonized with Human Serum Antibodies. PLoS ONE. 5, e9871, doi:10.1371/journal.pone.0009871.t003 (2010).
12. Kumaratilake, L. M., & Ferrante, A. Opsonization and phagocytosis of Plasmodium falciparum merozoites measured by flow cytometry. Clinical and diagnostic laboratory immunology. 7, 9-13 (2000).
13. Richards, J. S. et al. Identification and Prioritization of Merozoite Antigens as Targets of Protective Human Immunity to Plasmodium falciparum Malaria for Vaccine and Biomarker Development. The Journal of Immunology, doi:10.4049/jimmunol.1300778 (2013).
14. Salmon, B. L., Oksman, A., & Goldberg, D. E. Malaria parasite exit from the host erythrocyte: a two-step process requiring extraerythrocytic proteolysis. Proceedings of the National Academy of Sciences of the United States of America. 98, 271-276, doi:10.1073/pnas.011413198 (2001).
15. Boyle, M. J. et al. Isolation of viable Plasmodium falciparum merozoites to define erythrocyte invasion events and advance vaccine and drug development. Proceedings of the National Academy of Sciences. 107, 14378-14383, doi:10.1073/pnas.1009198107 (2010).
16. Wilson, D. W., Langer, C., Goodman, C. D., Mcfadden, G. I., & Beeson, J. G. Defining the timing of action of anti-malarial drugs against Plasmodium falciparum. Antimicrobial Agents and Chemotherapy, 1-46, doi:10.1128/AAC.01881-12 (2013).
17. Angrisano, F. et al. Spatial Localisation of Actin Filaments across Developmental Stages of the Malaria Parasite. PLoS ONE. 7, e32188, doi:10.1371/journal.pone.0032188.s010 (2012).
18. Riglar, D. T. et al. Super-resolution dissection of coordinated events during malaria parasite invasion of the human erythrocyte. Cell Host and Microbe. 9, 9-20, doi:10.1016/j.chom.2010.12.003 (2011).
19. Zuccala, E. S. et al. Subcompartmentalisation of Proteins in the Rhoptries Correlates with Ordered Events of Erythrocyte Invasion by the Blood Stage Malaria Parasite. PLoS ONE. 7, e46160, doi:10.1371/journal.pone.0046160.s004 (2012).
20. Marapana, D. S. et al. Malaria Parasite Signal Peptide Peptidase is an ER-Resident Protease Required for Growth but not for Invasion. Traffic. 13, 1457-1465, doi:10.1111/j.1600-0854.2012.01402.x (2012).
21. Rzepczyk, C. M., Lopez, J. A., Anderson, K. L., & Alpers, M. P. Investigation of the effect of monocytes with Papua New Guinea sera on Plasmodium falciparum in culture. International Journal for Parasitology. 18, 401-406 (1988).
22. Schofield, L., & Mueller, I. Clinical immunity to malaria. Curr Mol Med. 6, 205-221 (2006).
23. Hill, D. L. et al. Efficient measurement of opsonizing antibodies to Plasmodium falciparum merozoites. PLoS ONE. 7, e51692, doi:10.1371/journal.pone.0051692 (2012).
24. Tsuchiya, S. et al. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). International journal of cancer Journal international du cancer. 26, 171-176 (1980).
25. Ackerman, M. E. et al. A robust, high-throughput assay to determine the phagocytic activity of clinical antibody samples. Journal of Immunological Methods. 366, 8-19, doi:10.1016/j.jim.2010.12.016 (2011).
26. Ataíde, R. et al. Using an Improved Phagocytosis Assay to Evaluate the Effect of HIV on Specific Antibodies to Pregnancy-Associated Malaria. PLoS ONE. 5, e10807, doi:10.1371/journal.pone.0010807.t003 (2010).
27. Wilson, D. W., Crabb, B. S., & Beeson, J. G. Development of fluorescent Plasmodium falciparum for in vitro growth inhibition assays. Malar J. 9, 152, doi:1475-2875-9-152 [pii] 10.1186/1475-2875-9-152 (2010).
28. Lambros, C., & Vanderberg, J. P. Synchronization of Plasmodium falciparum erythrocytic stages in culture. J Parasitol. 65, 418-420 (1979).
29. Braun-Breton, C., Rosenberry, T. L., & da Silva, L. P. Induction of the proteolytic activity of a membrane protein in Plasmodium falciparum by phosphatidyl inositol-specific phospholipase C. Nature. 332, 457-459, doi:10.1038/332457a0 (1988).
30. Jaramillo, M., Godbout, M., & Olivier, M. Hemozoin induces macrophage chemokine expression through oxidative stress-dependent and -independent mechanisms. J Immunol. 174, 475-484 (2005).
31. Schofield, L., & Tachado, S. D. Regulation of host cell function by glycosylphosphatidylinositols of the parasitic protozoa. Immunology and cell biology. 74, 555-563, doi:10.1038/icb.1996.89 (1996).
32. Khusmith, S., Druilhe, P., & Gentilini, M. Enhanced Plasmodium falciparum merozoite phagocytosis by monocytes from immune individuals. Infection and immunity. 35, 874-879 (1982).
33. Lunov, O. et al. Differential uptake of functionalized polystyrene nanoparticles by human macrophages and a monocytic cell line. ACS nano. 5, 1657-1669, doi:10.1021/nn2000756 (2011).
34. Tebo, A. E., Kremsner, P. G., & Luty, A. J. Fcgamma receptor-mediated phagocytosis of Plasmodium falciparum-infected erythrocytes in vitro. Clinical and experimental immunology. 130, 300-306 (2002).
35. McGilvray, I. D., Serghides, L., Kapus, A., Rotstein, O. D., & Kain, K. C. Nonopsonic monocyte/macrophage phagocytosis of Plasmodium falciparum-parasitized erythrocytes: a role for CD36 in malarial clearance. Blood. 96, 3231-3240 (2000).