Baicalin protects sertoli cells from heat stress-induced apoptosis via activation of the Fas/FasL pathway and Hsp72 expression

Reproductive Toxicology

Volumn 57, Issue , 2015, Pages 196-203

  Guo, Xiaotong ^a   Chi, Shikai ^a   Cong, Xia ^a   Li, Huatao ^a   Jiang, Zhongling ^a   Cao, Rongfeng ^a   Tian, Wenru ^a  

^a QINGDAO AGRICULTURAL UNIVERSITY   (China)

Author keywords

Apoptosis; Baicalin; Bovine sertoli cells; Heat stress; Hsp72

Indexed keywords

BAICALIN; CASPASE 3; CASPASE 8; FAS ANTIGEN; FAS LIGAND; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HEAT SHOCK PROTEIN 72; FLAVONOID; MESSENGER RNA; PROTECTIVE AGENT;

ANIMAL CELL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; BOVINE; CELL PROTECTION; CELL SURVIVAL; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG ACTIVITY; DRUG STRUCTURE; ENZYME ACTIVITY; HEAT STRESS; MALE; NONHUMAN; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; SERTOLI CELL; SIGNAL TRANSDUCTION; UPREGULATION; ADVERSE EFFECTS; ANIMAL; CELL CULTURE; DRUG EFFECTS; GENETICS; HEAT; METABOLISM;

ANIMALIA; BOVINAE;

ANIMALS; ANTIGENS, CD95; APOPTOSIS; CASPASE 3; CATTLE; CELLS, CULTURED; FAS LIGAND PROTEIN; FLAVONOIDS; HOT TEMPERATURE; HSP72 HEAT-SHOCK PROTEINS; MALE; PROTECTIVE AGENTS; RNA, MESSENGER; SERTOLI CELLS; SIGNAL TRANSDUCTION;

EID: 84939479775     PISSN: 08906238     EISSN: 18731708     Source Type: Journal    
DOI: 10.1016/j.reprotox.2015.06.049     Document Type: Article

References (44)

1. Setchell B.P. The parkes lecture. Heat and the testis. J Reprod Fertil 1998, 114:179-194. 10.1530/jrf.0.1140179.
2. Bantwal G., Mathew V. Male contraception. J Indian J Endocrinol Metab 2012, 16(6):910-917. 10.4103/2230-8210.102991.
3. Lue Y.H., Hikim A.P., Swerdloff R.S., Im P., Taing K.S., Bui T., et al. J Endocrinol 1999, 140:1709-1717. 10.1210/endo.140.4.6629.
4. Brito L.F., Silva A.E., Barbosa R.T., Kastelic J.P. J Theriogenol 2004, 61(2-3):511-528. 10.1016/S0093-691X(03)00231-0.
5. Durairajanayagam D., Agarwal A., Ong C. Causes, effects and molecular mechanisms of testicular heat stress. J Reprod Biomed Online 2015, 30(1):14-27. 10.1016/j.rbmo.2014.09.018.
6. Cai H., Ren Y., Li X.X., Yang J.L., Zhang C.P., Chen M., et al. Scrotal heat stress causes a transient alteration in tight junctions and induction of TGF-beta expression. Int J Androl 2011, 34:352-362. 10.1111/j.1365-2605.2010.01089.x.
7. Nair R., Shaha C. Diethylstilbestrol induces rat spermatogenic cell apoptosis in vivo through increased expression of spermatogenic cell Fas/FasL system. J Biol Chem 2003, 278(8):6470-6481. 10.1074/jbc.M209319200.
8. Qi S., Fu W., Wang C., et al. BPA-induced apoptosis of rat Sertoli cells through Fas/FasL and JNKs/p38 MAPK pathways. J Reprod Toxicol 2014, 50:108-116. 10.1016/j.reprotox.2014.10.013.
9. Nezhad F.S., Lavvaf A., Ahmadi A.Y. Studying effect of heat stress on DNA damage exposure in Sertoli cells. J Eur J Zool Res 2013, 2(6):70-74. ISSN: 2278-7356.
10. Assinder S., Davis R., Fenwick M., Glover A. Adult-only exposure of male rats to a diet of high phytoestrogen content increases apoptosis of meiotic and post-meiotic germ cells. J Reprod 2007, 133(1):11-19. 10.1530/rep.1.01211.
11. Wechalekar H., Setchell B.P., Peirce E.J., Ricci M., Leigh C., Breed W.G. Whole-body heat exposure induces membrane changes in spermatozoa from the cauda epididymidis of laboratory mice. J Asian J Androl 2010, 12:591-598. 10.1038/aja.2010.41.
12. Dix D.J., Allen J.W., Collins B.W., Poorman-Allen P., Mori C., Blizard D.R., et al. Hsp70-2 is required for desynapsis of synaptonemal complexes during meiotic prophase in juvenile and adult mouse spermatocytes. J Dev 1997, 124:4595-4603. http://dev.biologists.org/content/124/22/4595.long.
13. Kiang J.G., Tsokos G.C. Heat shock protein 70kDa: molecular biology, biochemistry, and physiology. J Pharmacol Therap 1998, 80(2):183-201. http://www.sciencedirect.com/science/article/pii/S016372589800028X.
14. Chen M., Yuan J., Shi Y. Effect of 43°C treatment on expression of heat shock proteins 105, 70 and 60 in cultured monkey Sertoli cells. J Asian J Androl 2008, 10:474-485. 10.1111/j.1745-7262.2008.00391.x.
15. Dix D.J., Allen J.W., Collins B.W., Goulding E.H., Eddy E.M. Targeted gene disruption of hsp70-2 results in failed meiosis, germ cell apoptosis, and male infertility. J Sci Proc Natl Acad 1996, 93:3264-3268.
16. Huang W.H., Lee A.R., Yang C.H. Antioxidative and anti-inflammatory activities of polyhydroxyflavonoids of Scutellaria baicalensis GEORGI. J Biosci Biotechnol Biochem 2006, 70(10):2371-2380. 10.1271/bbb.50698.
17. Xu S., Meng Q.G. Advance of research on antipyretic effect and mechanism of Scutellaria. J Chin Arch Tradit Chin Med 2008, 06:1179-1181. 10.13193/j.archtcm.2008.06.60.xush.057.
18. Li Q.N., Ge X.Q. Effect of baicalin on antipyresis and influence on cytokine. J China J Chin Mater Med 2010, 35(4):1068-1072. 10.4268/cjcmm20100829.
19. Huang Y., Tsang S.Y., Yao X., Chen Z.Y. Biological properties of baicalein in cardiovascular system. J Curr Drug Targets - Cardiovasc Hematol Disord 2005, 5(2):177-184. 10.3969/j.issn.1003-6245.2010.05.022.
20. Song L.L., Meng Q.G. Study development on phar macodynamics effect of scutellaria. J Zhong hua Zhong yi yao Xue kan 2008, 26(8):29. 10.13193/j.archtcm.2008.08.77.songll.067.
21. Liu L.L., Gong L.K., Wang H., Xiao Y., Wu X.F., Zhang Y.H., et al. Baicalin protects mouse from concanavalin A-induced liver injury through inhibition of cytokine production and hepatocyte apoptosis. J Liver Int 2007, 27(4):582-591. ISSN: 1478-3223.
22. Guo X., Zhang Q., et al. Baicalin effects glial cell line-derived neurotrophic factor (GDNF) and stem cell factors (SCF) expression in heat-stressed calf (Bos taurus) Sertoli cells. J Agric Biotechnol 2015, 04:441-449. 10.3969/j.issn.1674-7968.2015.04.003.
23. Zhang H., Liu B., Qiu Y. Pure cultures and characterization of yak Sertoli cells. J Tissue Cell 2013, 45(6):414-420. 10.1016/j.tice.2013.07.004.
24. Barekzi N., Laroussi M. Dose-dependent killing of leukemia cells by low-temperature plasma. J Phys D: Appl Phys 2012, 45(42):422002. 10.1088/0022-3727/45/42/422002.
25. Liu C., Zhao X.-E., Ma B.-H., Sun B. Efficient separation, culture and identification of Sertoli cells in new-born calf. J Northwest A F Univ 2010, 38:6-10.
26. Hao M.C., Liu B., Fan J.F., Wang, et al. Establishment and characterization of a yak mammary myoepithelial cell line (YMM). J Anim Vet Adv 2012, 11:1028-1035. [eCollection 2014]. 10.1371/journal.pone.0113669.
27. Zhang B.Z., Guo X.T., Chen J.W., Zhao Y., Cong X., Jiang Z.L., et al. Saikosaponin-D attenuates heat stress-induced oxidative damage in LLC-PK1 cells by increasing the expression of anti-oxidant enzymes and HSP72. J Am J Chin Med 2014, 42(05):1261-1277. 10.1142/S0192415X14500797.
28. Schmittgen T.D., Livak K.J. Analyzing real-time PCR data by the comparative CT method. J Nat Protoc 2008, 3(6):1101-1108. 10.1038/nprot.2008.73.
29. Imao M., Nagaki M., Moriwaki H. Dual effects of heat stress on tumor necrosis factor-alpha-induced hepatocyte apoptosis in mice. J Lab Investig 2006, 86:959-967. doi:10.1038%2Flabinvest.3700451.
30. Kanter M., Aktas C., Erboga M. Heat stress decreases testicular germ cell proliferation and increases apoptosis in short term: an immunohistochemical and ultrastructural study. J Toxicol Ind Health 2013, 29:99-113. 10.1177/0748233711425082.
31. Simões V., Alves M., Martins A., Dias T., Rato L., Socorro S., et al. Regulation of apoptotic signaling pathways by 5 α-dihydrotestosterone and 17β-estradiol in immature rat Sertoli cells. J Steroid Biochem Mol Biol 2013, 135:15-23. 10.1016/j.jsbmb.2012.11.019.
32. Cai H., Ren Y., Li X.X., Yang J.L., Zhang C.P., Chen M., et al. Scrotal heat stress causes a transient alteration in tight junctions and induction of TGF-β expression. J Int J Androl 2011, 34(4pt1):352-362. 10.1111/j.1365-2605.2010.01089.x.
33. Ai X., Butts B., Vora K., Li W., Tache-Talmadge C., Fridman A., et al. Generation and characterization of antibodies specific for caspase-cleaved neo-epitopes: a novel approach. J Cell Death Dis 2011, 2:e205. 10.1038/cddis.2011.91.
34. Lavrik I.N., Krammer P.H. Regulation of CD95/Fas signaling at the DISC. J Cell Death Differ 2012, 19:36-41. [Epub 2011 November 11]. 10.1038/cdd.2011.155.
35. Aitken R.J., Curry B.J. Redox regulation of human sperm function: from the physiological control of sperm capacitation to the etiology of infertility and DNA damage in the germ line. J Antioxid Redox Signal 2011, 14(3):367-381. 10.1089/ars.2010.3186.
36. Chan F.L., Choi H.L., Chen Z.Y., Chan P.S.F., Huang Y. Induction of apoptosis in prostate cancer cell lines by a flavonoid, baicalin. J Cancer Lett 2000, 160(2):219-228. http://www.ncbi.nlm.nih.gov/pubmed/11053652.
37. Ye L., Tao Y., Wang Y., Feng T., Li H. The effects of baicalin on the TLR2/4 signaling pathway in the peripheral blood mononuclear cells of a lipopolysaccharide-induced rat fever model. J Int Immunopharmacol 2015, 10.1016/j.intimp.2014.12.028.
38. Sun C.-L., Guo X.-T., Zhao Y., Chen J.-W., Cong X., Wang X., et al. Effects of baicalin on expression of B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X Protein Gene (Bax) and apoptosis rates of pig kidney proximal tubular (LLC-PK1) cells subjected to heat stress. J Agric Biotechnol 2014, 12:1553-1560. 10.3969/j.issn.1674-7968.2014.12.010.
39. Cao Y., Mao X., Sun C., Zheng P., Gao J., Wang X., et al. Baicalin attenuates global cerebral ischemia/reperfusion injury in gerbils via anti-oxidative and anti-apoptotic pathways. J Brain Res Bull 2011, 85(6):396-402. 10.1016/j.brainresbull.2011.05.002.
40. Shen M., Wang L., Yang G., Gao L., Wang B., Guo X., et al. Baicalin protects the cardiomyocytes from ER stress-induced apoptosis: inhibition of CHOP through induction of endothelial nitric oxide synthase. J PLoS ONE 2014, 9(2):e88389. [eCollection 2014]. 10.1371/journal.pone.0088389.
41. O'Neill S., Harrison E.M., Ross J.A., Wigmore S.J., Hughes J. Heat-shock proteins and acute ischaemic kidney injury. J Nephron Exp Nephrol 2014, 126(4):167-174. 10.1159/000363323.
42. Purandhar K., Jena P.K., Prajapati B., Rajput P., Seshadri S. Understanding the role of heat shock protein isoforms in male fertility, aging and apoptosis. J World J Men's Health 2014, 32(3):123-132. 10.5534/wjmh.2014.32.3.123.
43. Steel R., Doherty J.P., Buzzard K., Clemons N., Hawkins C.J., Anderson R.L. Hsp72 inhibits apoptosis upstream of the mitochondria and not through interactions with Apaf-1. J Biol Chem 2004, 279:51490-51499. 10.1074/jbc.M401314200.
44. Liu P., Wang J.-Y., Li Q., Wang Z.-Y., Liu Z.-P., Zhang X.-M. Effects of baicalin on neuronal apoptosis and related gene expression after focal cerebral ischemia-reperfusion injury in rats. J Chin J New Drugs Clin Remed 2007, 02:109-114.