Identification and Validation of Quantitative PCR Reference Genes Suitable for Normalizing Expression in Normal and Dystrophic Cell Culture Models of Myogenesis

PLoS Currents

Volumn , Issue MAR, 2014, Pages

  Hildyard, John C W ^a   Wells, Dominic J ^a  

^a ROYAL VETERINARY COLLEGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADAPTER RELATED PROTEIN COMPLEX 3 SUBUNIT; BETA ACTIN; CASEIN KINASE II; COMPLEMENTARY DNA; DYSTROPHIN; F BOX PROTEIN; GAMMA INTERFERON; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HIV 1 TAT SPECIFIC FACTOR; MESSENGER RNA; MYOCYTE ENHANCER FACTOR 2; MYOD PROTEIN; MYOGENIN; P21 ACTIVATED KINASE 1; PEPTIDES AND PROTEINS; RNA 18S; UNCLASSIFIED DRUG; ZINC FINGER PROTEIN;

ARTICLE; CELL CULTURE; CELL CYCLE PARAMETERS; CELL DIFFERENTIATION; CELL FUNCTION; CELL MIGRATION; CONTROLLED STUDY; DNA SYNTHESIS; GENE; GENETIC ANALYSIS; HUMAN; HUMAN CELL; IN VITRO STUDY; MUSCLE DEVELOPMENT; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; RNA PURIFICATION; SEQUENCE ANALYSIS;

EID: 84939259794     PISSN: None     EISSN: 21573999     Source Type: Journal    
DOI: 10.1371/currents.md.faafdde4bea8df4aa7d06cd5553119a6     Document Type: Article

References (23)

1. Thormar HG, Gudmundsson B, Eiriksdottir F, Kil S, Gunnarsson GH, Magnusson MK, Hsu JC, Jonsson JJ. Importance of the efficiency of double-stranded DNA formation in cDNA synthesis for the imprecision of microarray expression analysis. Clin Chem. 2013 Apr;59(4):667-74. PubMed PMID:23378568. 10.1373/clinchem.2012.19383923378568.
2. Rho HW, Lee BC, Choi ES, Choi IJ, Lee YS, Goh SH. Identification of valid reference genes for gene expression studies of human stomach cancer by reverse transcription-qPCR. BMC Cancer. 2010 May 28;10:240. PubMed PMID:20507635. 20507635.
3. Schmittgen TD, Zakrajsek BA. Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR. J Biochem Biophys Methods. 2000 Nov 20;46(1-2):69-81. PubMed PMID:11086195. 11086195.
4. Thellin O, Zorzi W, Lakaye B, De Borman B, Coumans B, Hennen G, Grisar T, Igout A, Heinen E. Housekeeping genes as internal standards: use and limits. J Biotechnol. 1999 Oct 8;75(2-3):291-5. PubMed PMID:10617337. 10617337.
5. Lafontaine DL. A 9garbage can9 for ribosomes: how eukaryotes degrade their ribosomes. Trends Biochem Sci. 2010 May;35(5):267-77. PubMed PMID:20097077. 20097077.
6. Jackowiak P, Nowacka M, Strozycki PM, Figlerowicz M. RNA degradome--its biogenesis and functions. Nucleic Acids Res. 2011 Sep 1;39(17):7361-70. PubMed PMID:21653558. 21653558.
7. Lodish H, Berk A, Zipursky SL,et al. Molecular Cell Biology. 4th edition. New York: W. H. Freeman; 2000.
8. Zhang J, Tang Z, Wang N, Long L, Li K. Evaluating a set of reference genes for expression normalization in multiple tissues and skeletal muscle at different development stages in pigs using quantitative real-time polymerase chain reaction. DNA Cell Biol. 2012 Jan;31(1):106-13. PubMed PMID:21631284. 21631284.
9. McBryan J, Hamill RM, Davey G, Lawlor P, Mullen AM. Identification of suitable reference genes for gene expression analysis of pork meat quality and analysis of candidate genes associated with the trait drip loss. Meat Sci. 2010 Oct;86(2):436-9. PubMed PMID:20579813. 20579813.
10. Bower NI, Johnston IA. Selection of reference genes for expression studies with fish myogenic cell cultures. BMC Mol Biol. 2009 Aug 10;10:80. PubMed PMID:19664261. 19664261.
11. McCurley AT, Callard GV. Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment. BMC Mol Biol. 2008 Nov 12;9:102. PubMed PMID:19014500. 19014500.
12. Muráni E, Murániová M, Ponsuksili S, Schellander K, Wimmers K. Identification of genes differentially expressed during prenatal development of skeletal muscle in two pig breeds differing in muscularity. BMC Dev Biol. 2007 Oct 1;7:109. PubMed PMID:17908293. 17908293.
13. Masilamani TJ, Loiselle JJ, Sutherland LC. Assessment of Reference Genes for Real-Time Quantitative PCR Gene Expression Normalization During C2C12 and H9c2 Skeletal Muscle Differentiation. Mol Biotechnol. 2013 Oct 22. PubMed PMID:24146429. 24146429.
14. Stern-Straeter J, Bonaterra GA, Hörmann K, Kinscherf R, Goessler UR. Identification of valid reference genes during the differentiation of human myoblasts. BMC Mol Biol. 2009 Jul 2;10:66. PubMed PMID:19573231. 19573231.
15. Yaffe D, Saxel O. Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle. Nature. 1977 Dec 22-29;270(5639):725-7. PubMed PMID:563524. 563524.
16. Muses S, Morgan JE, Wells DJ. A new extensively characterised conditionally immortal muscle cell-line for investigating therapeutic strategies in muscular dystrophies. PLoS One. 2011;6(9):e24826. PubMed PMID:21935475. 21935475.
17. Morgan JE, Beauchamp JR, Pagel CN, Peckham M, Ataliotis P, Jat PS, Noble MD, Farmer K, Partridge TA. Myogenic cell lines derived from transgenic mice carrying a thermolabile T antigen: a model system for the derivation of tissue-specific and mutation-specific cell lines. Dev Biol. 1994 Apr;162(2):486-98. PubMed PMID:8150209. 8150209.
18. PrimerDesign geNorm system.
19. Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations. Biotechnol Lett. 2004 Mar;26(6):509-15. PubMed PMID:15127793. 15127793.
20. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002 Jun 18;3(7):RESEARCH0034. PubMed PMID:12184808. 12184808.
21. Andersen CL, Jensen JL, Ørntoft TF. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 2004 Aug 1;64(15):5245-50. PubMed PMID:15289330. 15289330.
22. Tennyson CN, Klamut HJ, Worton RG. The human dystrophin gene requires 16 hours to be transcribed and is cotranscriptionally spliced. Nat Genet. 1995 Feb;9(2):184-90. PubMed PMID:7719347. 7719347.
23. Edmondson DG, Lyons GE, Martin JF, Olson EN. Mef2 gene expression marks the cardiac and skeletal muscle lineages during mouse embryogenesis. Development. 1994 May;120(5):1251-63. PubMed PMID:8026334. 8026334.