Ketone bodies effectively compete with glucose for neuronal acetyl-CoA generation in rat hippocampal slices

NMR in Biomedicine

Volumn 28, Issue 9, 2015, Pages 1111-1116

  Valente Silva, Paula ^a   Lemos, Cristina ^a   Köfalvi, Attila ^a   Cunha, Rodrigo A ^a,b   Jones, John G ^a,c  

^a UNIVERSITY OF COIMBRA   (Portugal)

^b UNIVERSITY OF COIMBRA   (Portugal)

^c APDP Portuguese Diabetes Association   (Portugal)

Author keywords

13C MRS; Energy metabolism; Ketogenic substrate; Lactate

Indexed keywords

KETONES; METABOLISM; NEURONS; RATS;

3-HYDROXYBUTYRATE; ENERGY GENERATIONS; ENERGY METABOLISM; HIPPOCAMPAL SLICE; ISOTOPOMER ANALYSIS; LACTATE; NON STEADY-STATE; ^13C-MRS;

GLUCOSE;

3 HYDROXYBUTYRIC ACID; 4 AMINOBUTYRIC ACID; 4 AMINOBUTYRIC ACID RECEPTOR; ACETYL COENZYME A; GLUCOSE; GLUTAMIC ACID; KETONE BODY; LACTIC ACID; PYRUVATE KINASE; PYRUVIC ACID; ACETIC ACID DERIVATIVE;

ANIMAL TISSUE; ARTICLE; BRAIN SLICE; CARBON NUCLEAR MAGNETIC RESONANCE; CITRIC ACID CYCLE; DIET RESTRICTION; ENERGY RESOURCE; GLYCOLYSIS; HIPPOCAMPUS; KETOGENIC DIET; MALE; NONHUMAN; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; RAT; ANIMAL; BIOSYNTHESIS; METABOLISM; NERVE CELL; WISTAR RAT;

RATTUS;

ACETATES; ACETYL COENZYME A; ANIMALS; CITRIC ACID CYCLE; GLUCOSE; GLYCOLYSIS; HIPPOCAMPUS; KETONE BODIES; MALE; NEURONS; PYRUVIC ACID; RATS; RATS, WISTAR;

EID: 84939190356     PISSN: 09523480     EISSN: 10991492     Source Type: Journal    
DOI: 10.1002/nbm.3355     Document Type: Article

References (28)

1. Owen OE, Morgan AP, Kemp HG, Sullivan JM, Herrera MG, Cahill GF. Brain metabolism during fasting. J. Clin. Invest. 1967; 46: 1589-1595.
2. Jiang L, Mason GF, Rothman DL, de Graaf RA, Behar KL. Cortical substrate oxidation during hyperketonemia in the fasted anesthetized rat in vivo. J. Cereb. Blood Flow Metab. 2011; 31: 2313-2323.
3. Pull I, McIlwain H. 3-Hydroxybutyrate dehydrogenase of rat brain on dietary change and during maturation. J. Neurochem. 1971; 18: 1163-1165.
4. Lutas A, Yellen G. The ketogenic diet: metabolic influences on brain excitability and epilepsy. Trends Neurosci. 2013; 36: 32-40.
5. Costantini LC, Barr LJ, Vogel JL, Henderson ST. Hypometabolism as a therapeutic target in Alzheimer's disease. BMC Neurosci. 2008; 9(Suppl. 2): S16.
6. Prins ML. Cerebral metabolic adaptation and ketone metabolism after brain injury. J. Cereb. Blood Flow Metab. 2008; 28: 1-16.
7. Lobley GE, Johnstone AM, Fyfe C, Horgan GW, Holtrop G, Bremner DM, Broom I, Schweiger L, Welch A. Glucose uptake by the brain on chronic high-protein weight-loss diets with either moderate or low amounts of carbohydrate. Br. J. Nutr. 2014; 111: 586-597.
8. Champ CE, Palmer JD, Volek JS, Werner-Wasik M, Andrews DW, Evans JJ, Glass J, Kim L, Shi W. Targeting metabolism with a ketogenic diet during the treatment of glioblastoma multiforme. J. Neuro-Oncol. 2014; 117: 125-131.
9. Dienel GA. Brain lactate metabolism: the discoveries and the controversies. J. Cereb. Blood Flow Metab. 2012; 32: 1107-1138.
10. Pellerin L. Food for thought: the importance of glucose and other energy substrates for sustaining brain function under varying levels of activity. Diabetes Metab. 2010; 36: S59-S63.
11. Chowdhury GMI, Jiang L, Rothman DL, Behar KL. The contribution of ketone bodies to basal and activity-dependent neuronal oxidation in vivo. J. Cereb. Blood Flow Metab. 2014; 34: 1233-1242.
12. Duarte JMN, Cunha RA, Carvalho RA. Different metabolism of glutamatergic and gabaergic compartments in superfused hippocampal slices characterized by nuclear magnetic resonance spectroscopy. Neuroscience 2007; 144: 1305-1313.
13. 13C NMR under non-steady-state conditions. Biochemistry 1990; 29: 6756-6761.
14. Lopes da Silva FH, Arnolds D. Physiology of hippocampus and related structures. Annu. Rev. Physiol. 1978; 40: 185-216.
15. Cooper AJL, Plum F. Biochemistry and physiology of brain ammonia. Physiol. Rev. 1987; 67: 440-519.
16. Daikhin Y, Yudkoff M. Compartmentation of brain glutamate metabolism in neurons and glia. J. Nutr. 2000; 130: 1026S-1031S.
17. Martin SJ, Grimwood PD, Morris RGM. Synaptic plasticity and memory: an evaluation of the hypothesis. Annu. Rev. Neurosci. 2000; 23: 649-711.
18. Lynch MA. Long-term potentiation and memory. Physiol. Rev. 2004; 84: 87-136.
19. Edmond J, Robbins RA, Bergstrom JD, Cole RA, Devellis J. Capacity for substrate utilization in oxidative-metabolism by neurons, astrocytes, and oligodendrocytes from developing brain in primary culture. J. Neurosci. Res. 1987; 18: 551-561.
20. Brooks KJ, Clark JB, Bates TE. 3-hydroxybutyrate aids the recovery of the energy state from aglycaemic hypoxia of adult but not neonatal rat brain slices. J. Neurochem. 1998; 70: 1986-1990.
21. Sato K, Kashiwaya Y, Keon CA, Tsuchiya N, King MT, Radda GK, Chance B, Clarke K, Veech RL. Insulin, ketone-bodies, and mitochondrial energy transduction. FASEB J. 1995; 9: 651-658.
22. Kashiwaya Y, Pawlosky R, Markis W, King MT, Bergman C, Srivastava S, Murray A, Clarke K, Veech RL. A ketone ester diet increases brain malonyl-CoA and uncoupling proteins 4 and 5 while decreasing food intake in the normal Wistar rat. J. Biol. Chem. 2010; 285: 25950-25956.
23. Melo TM, Nehlig A, Sonnewald U. Neuronal-glial interactions in rats fed a ketogenic diet. Neurochem. Int. 2006; 48: 498-507.
24. Waniewski RA, Martin DL. Preferential utilization of acetate by astrocytes is attributable to transport. J. Neurosci. 1998; 18: 5225-5233.
25. Qu H, Haberg A, Haraldseth O, Unsgard G, Sonnewald U. C-13 MR spectroscopy study of lactate as substrate for rat brain. Dev. Neurosci. 2000; 22: 429-436.
26. 13C NMR-spectroscopy. NMR Biomed. 1993; 6: 264-277.
27. Chowdhury GMI, Patel AB, Mason GF, Rothman DL, Behar KL. Glutamatergic and GABAergic neurotransmitter cycling and energy metabolism in rat cerebral cortex during postnatal development. J. Cereb. Blood Flow Metab. 2007; 27: 1895-1907.
28. Patel AB, de Graaf RA, Mason GF, Rothman DL, Shulman RG, Behar KL. The contribution of GABA to glutamate/glutamine cycling and energy metabolism in the rat cortex in vivo. Proc. Natl. Acad. Sci. U. S. A. 2005; 102: 5588-5593.