Characterization of silk fibroin/chitosan 3D porous scaffold and in vitro cytology

PLoS ONE

Volumn 10, Issue 6, 2015, Pages

  Zeng, Shuguang ^a   Liu, Lei ^a,b   Shi, Yong ^a   Qiu, Junqi ^a   Fang, Wei ^a   Rong, Mingdeng ^a   Guo, Zehong ^a   Gao, Wenfeng ^a  

^a SOUTHERN MEDICAL UNIVERSITY   (China)

^b Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CHITOSAN; MOLECULAR SCAFFOLD; NANOCOMPOSITE; NANOMATERIAL; POROUS POLYMER; SILK FIBROIN; ALKALINE PHOSPHATASE; BIOMATERIAL; FIBROIN;

ARTICLE; BIOCOMPATIBILITY; BONE GROWTH; CELL ADHESION; CELL GROWTH; CELL PROLIFERATION; CELLULAR SECRETION; CHEMICAL MODIFICATION; COMPOSITE MATERIAL; CROSS LINKING; CYTOLOGY; DEGRADATION; EXTRACELLULAR MATRIX; FREEZE DRYING; HUMAN; HUMAN CELL; IN VITRO STUDY; MOLECULAR SIZE; MOLECULAR STABILITY; OSTEOBLAST; POROSITY; TISSUE ENGINEERING; WATER ABSORPTION; BONE DEVELOPMENT; CELL LINE; CHEMISTRY; DRUG EFFECTS; FLUORESCENCE MICROSCOPY; INFRARED SPECTROSCOPY; METABOLISM; SCANNING ELECTRON MICROSCOPY; TISSUE SCAFFOLD;

ALKALINE PHOSPHATASE; BIOCOMPATIBLE MATERIALS; CELL ADHESION; CELL LINE; CELL PROLIFERATION; CHITOSAN; FIBROINS; HUMANS; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, FLUORESCENCE; OSTEOBLASTS; OSTEOGENESIS; POROSITY; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; TISSUE ENGINEERING; TISSUE SCAFFOLDS;

EID: 84939178820     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0128658     Document Type: Article

References (41)

1. Meinel L, Fajardo R, Hofmann S, Langer R, Chen J, Snyder B, et al. Silk implants for the healing of critical size bone defects. Bone, 2005, 37(5):688-698. PMID: 16140599
2. Altman GH, Diaz F, Jakuba C, Calabro T, Horan RL, Chen J, et al. Silk-basedbiomaterials. Biomaterials, 2003, 24(3):401-416. PMID: 12423595
3. Lahiji A, Sohrabi A, Hungerford DS, Frondoza CG. Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chondrocytes. J Biomed Mater Res, 2000, 51(4):586-595. PMID: 10880106
4. Hofmann S, Hagenmuller H, Koch AM, Müller R, Vunjak Novakovic G, Kaplan DL, et al. Control of in vitro tissue- engineered bone-like structures using human mesenchymal stem cells and porous silk scaffolds. Biomaterials, 2007, 28(6): 1152-1162. PMID: 17092555
5. She Z, Jin C, Huang Z, Zhang B, Feng Q, Xu Y. Silk fibroin/chitosan scaffold:preparation, char-acterization, and culture with HepG2 cell. J Mater Sci Mater Med, 2008, 19(12):3545-3553. doi: 10.1007/s10856-008-3526-y PMID: 18622765
6. Liu H, Li X, Zhou G, Fan H, Fan Y. Electrospun sulfated silk fibroin nanofibrous scaffolds for vascular tissue engineering. Biomaterials, 2011, 32(15): 3784-3793 doi: 10.1016/j.biomaterials.2011.02.002 PMID: 21376391
7. Neves SC, Moreira Teixeira LS, Moroni L, Reis RL, Van Blitterswijk CA, Alves NM, et al. Chitosan/poly (epsilon-caprolact-one) blend scaffolds for cartilage repair. Biomaterials, 2011, 32(4):1068-1079. doi: 10.1016/j.biomaterials.2010.09.073 PMID: 20980050
8. Zhang K, Qian Y, Wang H, Fan L, Huang C, Mo X. Electrospun silk fibroin-hydroxybutyl chitosan nanofibrous scaffolds to biomimic extracellular matrix. J Biomater Sci Polym- Ed, 2011, 22(8):1069-1082. doi: 10.1163/092050610X498204 PMID: 20615313
9. Mandal BB, Park SH, Gil ES, Kaplan DL. Multilayered silk scaffolds for meniscus tissue engineering. Biomaterials, 2011, 32(2): 639-651 doi: 10.1016/j.biomaterials.2010.08.115 PMID: 20926132
10. Wang S, Zhang Y, Wang H, Dong Z. Preparation, characterization and biocomp-atibility of electrospinning heparin-modified silk fibroin nanofibers. Int J Biol Macromol, 2011, 48(2): 345-353. doi: 10.1016/j.ijbiomac.2010.12.008 PMID: 21182858
11. Dunne LW, Iyyanki T, Hubenak J, Mathur AB. Characterization of dielectrophoresis-aligned nanofibrous silk fibroin-chitosan scaffold and its interactions with endothelial cells for tissue engineering applications. Acta Biomater. 2014; 10(8):3630-40. doi: 10.1016/j.actbio.2014.05.005 PMID: 24821141
12. Gu Y, Zhu J, Xue C, Li Z, Ding F, Yang Y, et al. Chitosan/silk fibroin-based, Schwann cell-derived extracellular matrix-modified scaffolds for bridging rat sciatic nerve gaps. Biomaterials. 2014; 35(7):2253-63. doi: 10.1016/j.biomaterials.2013.11.087 PMID: 24360577
13. Guan L, Ge H, Tang X, Su S, Tian P, Xiao N, et al. Use of a silk fibroin-chitosan scaffold to construct a tissue-engineered corneal stroma. Cells Tissues Organs. 2013; 198(3):190-7. doi: 10.1159/000355944 PMID: 24247045
14. Yang MC, Wang SS, Chou NK, Chi NH, Huang YY, Chang YL, et al. The cardiomyogenic differentiation of rat mesenchymal stem cells on silk fibroin-polysaccharide cardiac patches in vitro. Biomaterials. 2009; 30(22):3757-65. doi: 10.1016/j.biomaterials.2009.03.057 PMID: 19410289
15. Chen JP, Chen SH, Lai GJ. Preparation and characterization of biomimetic silk fibroin/chitosan composite nanofibers by electrospinning for osteoblasts culture. Nanoscale Res Lett. 2012; 7(1):170 doi: 10.1186/1556-276X-7-170 PMID: 22394697
16. Lai GJ, Shalumon KT, Chen JP. Response of human mesenchymal stem cells to intrafibrillar nanohydroxyapatite content and extrafibrillar nanohydroxyapatite in biomimetic chitosan/silk fibroin/nanohydroxyapatite nanofibrous membrane scaffolds. Int J Nanomedicine. 2015; 10:567-84. doi: 10.2147/IJN.S73780 PMID: 25609962
17. Lai GJ, Shalumon KT, Chen SH, Chen JP. Composite chitosan/silk fibroin nanofibers for modulation of osteogenic differentiation and proliferation of human mesenchymal stem cells. Carbohydr Polym. 2014; 111:288-97 doi: 10.1016/j.carbpol.2014.04.094 PMID: 25037354
18. Boudriot U, Dersch R, Greiner A, Wendorff JH. Electrospinning approaches toward scaffold engineering - a brief overview. Artif Organs. 2006; 30(10):785-92. PMID: 17026578
19. Ji W, Zhang Y, Hu S, Zhang Y. Biocompatibility study of a silk fibroin-chitosan scaffold with adipose tissue-derived stem cells in vitro. Exp Ther Med. 2013; 6(2):513-518. PMID: 24137218
20. Deng J, She R, Huang W, Dong Z, Mo G, Liu B. A silk fibroin/chitosan scaffold in combination with bone marrow-derived mesenchymal stem cells to repair cartilage defects in the rabbit knee. J Mater Sci Mater Med. 2013; 24(8):2037-46. doi: 10.1007/s10856-013-4944-z PMID: 23677433
21. Tao W, Li M, Zhao C. Structure and properties of regenerated antheraea pernyi silk fibroin in aqueous solution. Int J Biol Macromol, 2007, 40(5): 472-478. PMID: 17173967
22. Nazarov R, Jin HJ, Kaplan DL. Porous 3-D scaffolds from regenerated silk fibroin. Biomacromolecules, 2004, 5(3):718-726. PMID: 15132652
23. Person FG, Marchessault RH, Liang CY. Infrared spectra of crystalline polysaccharides V.Chitinp. J Polym Sci. 1960; 43: 101-116.
24. Mikos AG, Sarakinos G, Leite SM, Vacanti JP, Langer R. Laminated three-dimensional biodeg-radable foams for use in tissue engineering. Biomaterials,1993, 14(5):323-330. PMID: 8507774
25. Mikos AG, Lyman MD, Freed LE, Langer R. Wetting of poly(L-lactic acid) and poly(DL-lactic-co-glycolic acid) foams for tissue culture. Biomaterials, 1994, 15 (1): 55-58. PMID: 8161659
26. Singh L, Kumar V, Ratner BD. Generation of porous microcellular 85/15 poly (DL-lactide-co-glycolide) foams for biomedical applications. Biomaterials, 2004, 25(13): 2611-2617. PMID: 14751747
27. Gerçek I, Tigli RS, Gümüşderelioglu M. A novel scaffold based on formation and agglomeration of PCL microbeads by freeze-drying. J Biomed Mater ReA, 2008, 86(4):1012-1022. PMID: 18067167
28. Kim TK, Yoon JJ, Lee DS, Park TG. Gas foamed open porous biodegradable polymeric microspheres. Biomaterials, 2006, 27(2):152-159. PMID: 16023197
29. Zhang K, Qian Y, Wang H, Fan L, Huang C, Yin A, et al. Genipin-crosslinked silk fibroin/hydroxybutyl chitosan nanofibrous scaffolds for tissue-engineering application. J Biomed Mater Res A, 2010, 95(3): 870-881. doi: 10.1002/jbm.a.32895 PMID: 20824649
30. Qazvini NT, Zinatloo S. Synthesis and characterization of gelatin nanoparticles using CDI/NHS as a non-toxic cross-linking system. J Mater Sci Mater Med, 2011, 22(1):63-69. doi: 10.1007/s10856-010-4178-2 PMID: 21052793
31. Harris LD, Kim BS, Mooney DJ. Open pore biodegradable matrices formed with gas foaming. Journal of Biomedical Materials Research, 1998, 42(3):396-402. PMID: 9788501
32. Sun K, Li H, Li R, Nian Z, Li D, Xu C. Silk fibroin/collagen and silk fibroin/chitosan blended three-dimensional scaffolds for tissue engineering. Eur J Orthop Surg Traumatol. 2015; 25(2):243-9. doi: 10.1007/s00590-014-1515-z PMID: 25118870
33. Elliott A, Ambrose EJ. Structure of synthetic polypeptides. Nature, 1950, 165(4206):921-922. PMID: 15423537
34. Yang F, Cui W, Xiong Z, Liu L, Bei J, Wang S. Poly(L,L-lactide-co-glycolide)/tricalcium phospate composite scaffold and its various changes during degradation in vitro. Polymer Degradation and Stability, 2006, 91(12):3065-3073.
35. Mittal A, Negi P, Garkhal K, Verma S, Kumar N. Integration of porosity and bio-functionalization to form a 3D scaffold: cell culture studies and in vitro degradation. Biomed Mater, 2010, 5(4):045001. doi: 10.1088/1748-6041/5/4/045001 PMID: 20539055
36. Kang Y, Yao Y, Yin G, Huang Z, Liao X, Xu X, et al. A study on the in vitro degradation properties of poly(L-lactic acid)/beta-tricalcuim phosphate (PLLA/beta-TCP) scaffold under dynamic loading. Med Eng Phys, 2009, 31(5):589-594. doi: 10.1016/j.medengphy.2008.11.014 PMID: 19131266
37. Horan RL, Antle K, Collette AL, Wang Y, Huang J, Moreau JE, et al. In vitro degradation of silk fibroin. Biomaterials, 2005, 26(17):3385-3393. PMID: 15621227
38. Gori F, Thomas KC, Hicok TC, Spelsberg TC, Riggs BL. Defferentiati of human marrow stromal precursor cells:bone morphogenetic protein-2 increases OSF2/CBFA1,enhances osteoblast commitment, and inhibits late adipocyte maturation. J Bone Miner Res, 1999, 14(9):1522-1535. PMID: 10469280
39. Bellows CG, Aubin JEM, Antosa ME. Mineralized bone nodules formedin vitrofrom enzymatically released rat calvaria populations. Calcif Tissue Int, 1986, 38(3):143-154. PMID: 3085892
40. Errico JA, Macneil RL, Takata T, Berry J, Strayhorn C, Somerman MJ. Expression of bone associated marker by tooth root lining cells in situ and vitro. Bone, 1997, 20 (2):117-126. PMID: 9028535
41. Collignon H, Davicco MJ, Barlet JP. Isolation of cells from oxine fetal long bone and characterization of their osteoblastic activities during in vitro mineralization. Arch Physiol Biochem, 1997, 105(2):158-166. PMID: 9255400