Preparation of Human Nuclear RNA m6A Methyltransferases and Demethylases and Biochemical Characterization of Their Catalytic Activity

Methods in Enzymology

Volumn 560, Issue , 2015, Pages 117-130

  Liu, Jianzhao ^a,b   Yue, Yanan ^a,b   He, Chuan ^a,b  

^a UNIVERSITY OF CHICAGO   (United States)

^b UNIVERSITY OF CHICAGO   (United States)

Author keywords

ALKBH5; Demethylation activity; FTO; m6A demethylase; m6A methyltransferase; Methylation activity; METTL3 METTL14; N6 methyladenosine

Indexed keywords

6 N METHYLADENOSINE; BACMID; DOUBLE STRANDED DNA; DOUBLE STRANDED RNA; LONG UNTRANSLATED RNA; NUCLEAR RNA; NUCLEAR RNA DEMETHYLASE; NUCLEAR RNA METHYLTRANSFERASE; PROTEIN ALKBH5; PROTEIN FTO; PROTEIN METTL14; PROTEIN METTL3; PROTEIN WTAP; RECOMBINANT PROTEIN; RNA METHYLTRANSFERASE; SINGLE STRANDED DNA; SINGLE STRANDED RNA; UNCLASSIFIED DRUG; ADENOSINE; ALKBH5 PROTEIN, HUMAN; DIOXYGENASE; FTO PROTEIN, HUMAN; MEMBRANE PROTEIN; MESSENGER RNA; METHYLTRANSFERASE; METTL14 PROTEIN, HUMAN; METTL3 PROTEIN, HUMAN; N(6)-METHYLADENOSINE; PROTEIN;

ANIMAL CELL; ARTICLE; BACTERIUM; BACULOVIRUS EXPRESSION SYSTEM; BIOCHEMICAL ANALYSIS; CONSENSUS SEQUENCE; CONTROLLED STUDY; DEMETHYLATION; DNA PROBE; ENZYME ACTIVITY; ENZYME ANALYSIS; GEL FILTRATION; GENE EXPRESSION SYSTEM; GENETIC RECOMBINATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INSECT CELL; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN METHYLATION; PROTEIN PURIFICATION; RNA PROBE; SF9 CELL LINE; TANDEM MASS SPECTROMETRY; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; ANALOGS AND DERIVATIVES; BIOSYNTHESIS; CATALYSIS; CHEMISTRY; GENETICS; HUMAN; ISOLATION AND PURIFICATION;

ADENOSINE; CATALYSIS; DIOXYGENASES; HUMANS; MEMBRANE PROTEINS; METHYLTRANSFERASES; PROTEINS; RNA, LONG NONCODING; RNA, MESSENGER; RNA, NUCLEAR;

EID: 84938990130     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/bs.mie.2015.03.013     Document Type: Chapter

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