A comparative method for protein extraction and 2-D gel electrophoresis from different tissues of Cajanus cajan

Frontiers in Plant Science

Volumn 6, Issue AUG, 2015, Pages

  Singh, Nisha ^a   Jain, Neha ^a   Kumar, Ram ^a   Jain, Ajay ^a   Singh, Nagendra K ^a   Rai, Vandna ^a  

^a NATIONAL RESEARCH CENTRE ON PLANT BIOTECHNOLOGY   (India)

Author keywords

2 DE; Pigeonpea; Proteomics; SDS PAGE; Tissue specific

Indexed keywords

EID: 84938831306     PISSN: None     EISSN: 1664462X     Source Type: Journal    
DOI: 10.3389/fpls.2015.00606     Document Type: Article

References (27)

1. Agrawal, G. K., Timperio, A. M., Zolla, L., Bansal, V., Shukla, R., and Rakwal, R. (2013). Biomarker discovery and applications for foods and beverages: proteomics to nanoproteomics. J. Proteomics93, 74-92. doi: 10. 1016/j. jprot. 2013. 04. 014.
2. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254. doi: 10. 1016/0003-2697(76)90527-3.
3. Carpentier, S. B., Witters, E., Laukens, K., Deckers, P., Swennen, R., and Panis, B. (2005). Preparation of protein extracts from recalcitrant plant tissues: an evaluation of different methods for two-dimensional gel electrophoresis analysis. Proteomics 5, 2497-2507. doi: 10. 1002/pmic. 200401222.
4. Chitra, U., Singh, U., and Venkateswara, R. P. (1996). Phytic acid, in vitro protein digestibility, dietary fiber and minerals of pulses as influenced by processing methods. Plant Foods Human Nutr. 49, 307-316. doi: 10. 1007/BF01091980.
5. Cho, K., Torres, N. L., Subramanyam, S., Deepak, S. A., Sardesai, N., and Han, O. (2006). Protein extraction/solubilization protocol for monocot and dicot plant gel-based proteomics. J. Plant Biol. 49, 413-420. doi: 10. 1007/BF03031120.
6. Damerval, C., De Vienne, D., Zivy, M., and Thiellement, H. (1986). Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat seedling proteins. Electrophoresis 7, 52-54. doi: 10. 1002/elps. 1150070108.
7. Devouge, V., Rogniaux, H., Nesi, N., Tessier, D., Gueguen, J., and Larre, C. (2007). Differential proteomic analysis of four near-Isogenic Brassica napus varieties bred for their erucic acid and glucosinolate contents. J. Proteome Res. 6, 1342-1353. doi: 10. 1021/pr060450b.
8. FAOSTAT. (2015). FAOSTAT Database. Available at: http://faostat. fao. org/site/339/default. aspx.
9. Hurkman, W. J., and Tanaka C. K. (1986). Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis. Plant Physiol. 81, 802-806. doi: 10. 1104/pp. 81. 3. 802.
10. Isaacson, T., Damasceno, C. M. B., Saravanan, R. S., He, Y., Catala., Saladie, M., et al. (2006). Sample extraction techniques for enhanced proteomic analysis of plant tissues. Nature Protocols1, 769-774. doi: 10. 1038/nprot. 2006. 102.
11. Laemmli, U. (1970). Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227, 680-685. doi: 10. 1038/227680a0.
12. Maldonado, A. M., Echevarria-Zomeno, S., Jean-Baptiste, S., Hernandez, M., and Jorrin-Novo, J. V. (2008). Evaluation of three different protocols of protein extraction for Arabidopsis thalianaleaf proteome analysis by two-dimensional electrophoresis. J. Proteomics. 71, 461-472. doi: 10. 1016/j. jprot. 2008. 06. 012.
13. Michaud, D., and Asselin, A. (1995). Application to plant proteins of gel electrophoretic methods. J. Chromatogr. 698, 263-279. doi: 10. 1016/0021-9673(94)00880-I.
14. Newsholme, S. J., Maleeft, B. F., Steiner, S., Anderson, N. L., and Schwartz, L. W. (2000). Two-dimensional electrophoresis of liver proteins: characterization of a drug-induced hepatomegaly in rats. Electrophoresis 21, 2122-2128. doi: 10. 1002/1522-2683(20000601).
15. Reddy, R. P., Rao, K. V., and Rao, N. G. P. (1979). Heterosis and combining ability in pigeonpea. Indian J. Gen. Plant Breed. 39, 240-246.
16. Santoni, V., Delarue, M., Caboche, M., and Bellini, C. (1997). A comparison of two-dimensional electrophoresis data with phenotypical traits in Arabidopsis leads to the identification of a mutant (cri1) that accumulates cytokinins. Planta 202, 62-69. doi: 10. 1007/s004250050103.
17. Saravanan, R. S., and Rose, J. K. C. (2004). A critical evaluation of sample extraction techniques for enhanced proteomic analysis of recalcitrant plant tissues. Proteomics 4, 2522-2532. doi: 10. 1002/pmic. 200300789.
18. Sharma, S., Agarwal, N., and Verma, P. (2011). Pigeon pea (Cajanus cajan L.): a hidden treasure of regime nutrition. J. Func. Environ. Bot. 1, 91-101. doi: 10. 5958/j. 2231-1742. 1. 2. 010.
19. Singh, N. K., Gupta, D. K., Jayaswal, P. K., Mahato, A. K., Dutta, S., Singh, S., et al. (2012). The first draft of the pigeonpea genome sequence. J. Plant Biochem. Biotechnol. 21, 98-112. doi: 10. 1007/s13562-011-0088-8.
20. Stalikas, C. D. (2007). Extraction, separation, and detection methods for phenolic acids and flavonoids. J. Sep. Sci. 30, 3268-3295. doi: 10. 1002/jssc. 200700261.
21. Varshney, R. K., Chen, W., Li, Y., Bharti, A. K., Saxena, R. K., Schlueter, J. A., et al. (2012). Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume crop of resource-poor farmers. Nat. Biotechnol. 30, 83-89. doi: 10. 1038/nbt. 2022.
22. Varshney, R. K., Penmetsa, R. V., Dutta, S., Kulwal, P. L., Saxena, R. K., Datta, S., et al. (2010). Pigeonpea genomics initiative (PGI): an international effort to improve crop productivity of pigeonpea (Cajanus cajan L.) Molecular Breeding 26, 393-408. doi: 10. 1007/s11032-009-9327-2.
23. Wang, W., Scali, M., Vignani, R., Spadafora, A., Sensi, E., Mazzuca, S., et al. (2003). Protein extraction for two-dimensional electrophoresis from olive leaf, a plant tissue containing high levels of interfering compounds. Electrophoresis 24, 2369-2375. doi: 10. 1002/elps. 200305500.
24. Wang, W., Tai, F., and Chen, S. (2008). Optimizing protein extraction from plant tissues for enhanced proteomics analysis. J. Sep. Sci. 31, 2032-2039. doi: 10. 1002/jssc. 200800087.
25. Wu, F. S., and Wang, M. Y. (1984). Extraction of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis from protease-rich plant tissues. Anal. Biochem. 139, 100-103. doi: 10. 1016/0003-2697(84)90394-4.
26. Wu, X., Xiong, E., Wang, W., Scali, M., and Cresti, M. (2014). Universal sample preparation method integrating trichloroacetic acid/acetone precipitation with phenol extraction for crop proteomic analysis. Nature Protocols 9, 362-374. doi: 10. 1038/nprot. 2014. 022.
27. Zargar, S. M., Nazir, M., Rai, V., Hajduch, M., Agrawal, G. K., and Rakwal, R. (2015). Towards a common bean proteome atlas: looking at the current state of research and the need for a comprehensive proteome. Front. Plant Sci. 27: 201. doi: 10. 3389/fpls. 2015. 00201.