Rapid establishment of droplet digital PCR for quantitative GMO analysis

European Food Research and Technology

Volumn 241, Issue 3, 2015, Pages 427-439

  Köppel, René ^a   Bucher, Thomas ^a  

^a Official Food Control Authority of the Canton of Zurich   (Switzerland)

Author keywords

Droplet digital PCR; Genetically modified; GMO; Quantification; Rapid

Indexed keywords

DROPS; GENETICALLY MODIFIED PLANTS; NUCLEIC ACIDS; UNCERTAINTY ANALYSIS;

DIGITAL PCR; GENETICALLY MODIFIED; GMO; QUANTIFICATION; RAPID;

POLYMERASE CHAIN REACTION;

EID: 84938749475     PISSN: 14382377     EISSN: 14382385     Source Type: Journal    
DOI: 10.1007/s00217-015-2475-1     Document Type: Article

References (14)

1. Finney DJ (1951) The estimation of bacterial densities from dilution series. J Hyg 49(1):26–35 (Lond)
2. Titus RG, Marchnd M, Boon T, Louis JA (1985) A limiting dilution assay for quantifying Leishmania major in tissues of infected mice. Parasite Immunol 7:545–555
3. Sanders R, Huggett JF, Bushell CA, Cowen S, Scott DJ, Foy CA (2011) Evaluation of digital PCR for absolute DNA quantification. Anal Chem 83(17):6474–6484
4. Pinheira LB, Coleman VA, Hindson CM, Herrmann J, Hindson BJ, Bhat S, Emsli KR (2012) Evaluation of a droplet digital polymerase chain reaction format for DNA copy number quantification. Anal Chem 84:1003–1011
5. White RA, Quake SR, Currb K (2012) Digital PCR provides absolute quantitation of viral load for an occult RNA virus. J Virol Methods 179(1):45–50
6. Gürtler P, Gerdes L (2014) Digitale Polymerasekettenreaktion, ddPCR; BIOspektrum | 06.14 | 20. Jahrgang pp 632–635
7. Hindson CM, Chevillet JR, Briggs HA, Gallichotte EN, Rufa IK, Benjamin J, Hindson R, Vessella L, Tewari M (2013) Absolute quantification by droplet digital PCR versus analog real-time PCR. Nat Methods 10:1003–1005
8. Miotke L, Lau BT, Rumma TR, Hanlee P, Ji HP (2014) High sensitivity detection and quantitation of DNA copy number and single nucleotide variants with single color droplet digital PCR. Anal Chem 86(5):2618–2624
9. Höhne M, Santisi C, Meyer R (2002) Real-time multiplex PCR: an accurate method for the detection and quantification of 35S-CaMV promoter in genetically modified maize-containing food. Eur Food Res Technol 215(1):59–64
10. Scholdberg TA, Norden TD, Nelson D, Jenkins RG (2009) Evaluating precision and accuracy when quantifying different endogenous control, reference genes in maize using real-time PCR. J Agric Food Chem 57(7):2903–2911
11. Burns MJ, Burrell AM, Foy CA (2010) The applicability of digital PCR for the assessment of detection limits in GMO analysis. Eur Food Res Technol 231(3):353–362
12. McDermott GP, Do D, Litterst CM, Maar D, Hindson CM, Steenblock ER, Legler TC, Jouvenot Y, Marrs SH, Bemis A, Shah P, Wong J, Wang S, Sally D, Javier L, Dinio T, Han C, Brackbill TP, Hodges SP, Ling Y, Klitgord N, Carman GJ, Berman JR, KoehlerR T, Hiddessen AL, Walse P, Bousse L, Tzonev S, Hefner E, Hindson BJ, Cauly TH, Hamby K, Patel VP, Regan JF, Wyatt PW, Karlin-Neumann GA, Stumbo DP, Lowe AJ (2013) Multiplexed target detection using DNA-Binding dye chemistry in droplet digital PCR. Anal Chem 85(23):11619–11627
13. Corbisier P, Bhat S, Partis L, Rui V, Xie D, Emslie KR (2010) Absolute quantification of genetically modified MON810 maize (Zea mays L.) by digital polymerase chain reaction. Anal Bioanal Chem 396(6):2143–2150
14. Mano J, Hatano S, Futo S, Yoshii J, Nakae H, Naito S, Takabatake R, Kitta K (2014) Development of a reference material of a single DNA molecule for the quality control of PCR testing. Anal Chem 86(17):8621–8627