Unrestricted modification search reveals lysine methylation as major modification induced by tissue formalin fixation and paraffin embedding

Proteomics

Volumn 15, Issue 15, 2015, Pages 2568-2579

  Zhang, Ying ^a,b,c   Muller, Markus ^b   Xu, Bo ^a,d   Yoshida, Yutaka ^a   Horlacher, Oliver ^b   Nikitin, Frederic ^b   Garessus, Samuel ^b   Magdeldin, Sameh ^a,e   Kinoshita, Naohiko ^a   Fujinaka, Hidehiko ^a,f   Yaoita, Eishin ^a   Hasegawa, Miki ^c   Lisacek, Frederique ^b   Yamamoto, Tadashi ^a,c  

^a NIIGATA UNIVERSITY GRADUATE SCHOOL OF MEDICAL AND DENTAL SCIENCES   (Japan)

^b SWISS INSTITUTE OF BIOINFORMATICS   (Switzerland)

^c NIIGATA UNIVERSITY   (Japan)

^d ETH ZURICH   (Switzerland)

^e Suez Canal University   (Egypt)

^f National Hospital Organization Niigata National Hospital   (Japan)

Author keywords

Bioinformatics; FFPE tissue; Lysine methylation; Open modification search; Spectral library

Indexed keywords

FORMALDEHYDE; LYSINE; PARAFFIN; FIXATIVE; PROTEOME;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL MODIFICATION; CLUSTER ANALYSIS; CRYOPRESERVATION; FORMALIN FIXED PARAFFIN EMBEDDED TISSUE; GENE FUNCTION; GENE ONTOLOGY; HUMAN; HUMAN TISSUE; KIDNEY PARENCHYMA; LASER MICRODISSECTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN METHYLATION; PROTEOMICS; REVERSED PHASE LIQUID CHROMATOGRAPHY; TANDEM MASS SPECTROMETRY; TISSUE PRESERVATION; CHEMISTRY; CLASSIFICATION; FROZEN SECTION; KIDNEY; LIQUID CHROMATOGRAPHY; METABOLISM; METHYLATION; PROCEDURES; REPRODUCIBILITY; TISSUE FIXATION;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, LIQUID; CLUSTER ANALYSIS; FIXATIVES; FORMALDEHYDE; FROZEN SECTIONS; HUMANS; KIDNEY; LYSINE; METHYLATION; PARAFFIN EMBEDDING; PROTEOME; PROTEOMICS; REPRODUCIBILITY OF RESULTS; TANDEM MASS SPECTROMETRY; TISSUE FIXATION;

EID: 84938739525     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.201400454     Document Type: Article

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