Flow cytometric measurement of respiratory burst activity and surface expression of neutrophils for septic patient prognosis

Cytometry Part B - Clinical Cytometry

Volumn 90, Issue 4, 2016, Pages 368-375

  Bae, Mi Hyun ^a   Park, So Hee ^a   Park, Chan Jeoung ^a   Cho, Eun Jung ^a   Lee, Bo Ra ^a   Kim, Young Jin ^a   Park, Sang Hyuk ^b   Cho, Young Uk ^a   Jang, Seongsoo ^a   Song, Dong Keun ^c   Hong, Sang Bum ^a  

^a University of Ulsan College of Medicine   (South Korea)

^b Pusan National University School of Medicine   (South Korea)

^c Hallym University   (South Korea)

Author keywords

CD10; CD16; CD64; flow cytometry; neutrophil; respiratory burst; sepsis

Indexed keywords

ANTIBIOTIC AGENT; CD16 ANTIGEN; CD64 ANTIGEN; COMMON ACUTE LYMPHOBLASTIC LEUKEMIA ANTIGEN;

ADULT; ANTIBIOTIC THERAPY; ANTIGEN EXPRESSION; ARTICLE; ARTIFICIAL VENTILATION; CONTINUOUS RENAL REPLACEMENT THERAPY; CONTROLLED STUDY; DISEASE SEVERITY; FEMALE; FLOW CYTOMETRY; FLUID RESUSCITATION; HUMAN; INNATE IMMUNITY; MAJOR CLINICAL STUDY; MALE; MIDDLE AGED; MORTALITY; NEUTROPHIL; PHENOTYPE; PREDICTION; PRIORITY JOURNAL; PROGNOSIS; PROSPECTIVE STUDY; RESPIRATORY BURST; SEPSIS; SEPTIC SHOCK; SURVIVOR; AGED; CYTOLOGY; IMMUNOLOGY; INTENSIVE CARE UNIT; PATHOLOGY; PHYSIOLOGY; PROCEDURES; VERY ELDERLY;

ADULT; AGED; AGED, 80 AND OVER; FEMALE; FLOW CYTOMETRY; HUMANS; INTENSIVE CARE UNITS; MALE; MIDDLE AGED; NEUTROPHILS; PROGNOSIS; RESPIRATORY BURST; SEPSIS;

EID: 84938703024     PISSN: 15524949     EISSN: 15524957     Source Type: Journal    
DOI: 10.1002/cyto.b.21274     Document Type: Article

References (29)

1. Smith JA. Neutrophils, host defense, and inflammation: A double-edged sword. J Leukoc Biol 1994;56:672–686.
2. Kaneko T, Stearns-Kurosawa DJ, Taylor F Jr, Twigg M, Osaki K, Kinasewitz GT, Peer G, Kurosawa S. Reduced neutrophil CD10 expression in nonhuman primates and humans after in vivo challenge with E. coli or lipopolysaccharide. Shock 2003;20:130–137.
3. Livaditi O, Kotanidou A, Psarra A, Dimopoulou I, Sotiropoulou C, Augustatou K, Papasteriades C, Armaganidis A, Roussos C, Orfanos SE, et al. Neutrophil CD64 expression and serum IL-8: Sensitive early markers of severity and outcome in sepsis. Cytokine 2006;36:283–290.
4. Brown KA, Brain SD, Pearson JD, Edgeworth JD, Lewis SM, Treacher DF. Neutrophils in development of multiple organ failure in sepsis. Lancet 2006;368:157–169.
5. Kuijpers TW, Tool AT, van der Schoot CE, Ginsel LA, Onderwater JJ, Roos D, Verhoeven AJ. Membrane surface antigen expression on neutrophils: A reappraisal of the use of surface markers for neutrophil activation. Blood 1991;78:1105–1111.
6. Orr Y, Wilson DP, Taylor JM, Bannon PG, Geczy C, Davenport MP, Kritharides L. A kinetic model of bone marrow neutrophil production that characterizes late phenotypic maturation. Am J Physiol Regul Integr Comp Physiol 2007;292:R1707–R1716.
7. Guerin E, Orabona M, Raquil MA, Giraudeau B, Bellier R, Gibot S, Bene MC, Lacombe F, Droin N, Solary E, et al. Circulating immature granulocytes with T-cell killing functions predict sepsis deterioration. Crit Care Med 2014;42:2007–2018.
8. Martens A, Eppink GJ, Woittiez AJ, Eidhof H, de Leij LF. Neutrophil function capacity to express CD10 is decreased in patients with septic shock. Crit Care Med 1999;27:549–553.
9. Drifte G, Dunn-Siegrist I, Tissieres P, Pugin J. Innate immune functions of immature neutrophils in patients with sepsis and severe systemic inflammatory response syndrome. Crit Care Med 2013;41:820–832.
10. Gibot S, Bene MC, Noel R, Massin F, Guy J, Cravoisy A, Barraud D, De Carvalho Bittencourt M, Quenot JP, Bollaert PE, et al. Combination biomarkers to diagnose sepsis in the critically ill patient. Am J Respir Crit Care Med 2012;186:65–71.
11. Danikas DD, Karakantza M, Theodorou GL, Sakellaropoulos GC, Gogos CA. Prognostic value of phagocytic activity of neutrophils and monocytes in sepsis. Correlation to CD64 and CD14 antigen expression. Clin Exp Immunol 2008;154:87–97.
12. Vowells SJ, Sekhsaria S, Malech HL, Shalit M, Fleisher TA. Flow cytometric analysis of the granulocyte respiratory burst: A comparison study of fluorescent probes. J Immunol Methods 1995;178:89–97.
13. Segal BH, Leto TL, Gallin JI, Malech HL, Holland SM. Genetic, biochemical, and clinical features of chronic granulomatous disease. Medicine (Baltimore) 2000;79:170–200.
14. Bruns T, Peter J, Hagel S, Herrmann A, Stallmach A. The augmented neutrophil respiratory burst in response to Escherichia coli is reduced in liver cirrhosis during infection. Clin Exp Immunol 2011;164:346–356.
15. Bone RC, Sprung CL, Sibbald WJ. Definitions for sepsis and organ failure. Crit Care Med 1992;20:724–726.
16. Cohen J. The immunopathogenesis of sepsis. Nature 2002;420:885–891.
17. Hotchkiss RS, Karl IE. The pathophysiology and treatment of sepsis. N Engl J Med 2003;348:138–150.
18. Hall MW, Knatz NL, Vetterly C, Tomarello S, Wewers MD, Volk HD, Carcillo JA. Immunoparalysis and nosocomial infection in children with multiple organ dysfunction syndrome. Intensive Care Med 2011;37:525–532.
19. Meisel C, Schefold JC, Pschowski R, Baumann T, Hetzger K, Gregor J, Weber-Carstens S, Hasper D, Keh D, Zuckermann H, et al. Granulocyte-macrophage colony-stimulating factor to reverse sepsis-associated immunosuppression: A double-blind, randomized, placebo-controlled multicenter trial. Am J Respir Crit Care Med 2009;180:640–648.
20. Marshall JC. Neutrophils in the pathogenesis of sepsis. Crit Care Med 2005;33:S502–S505.
21. Monneret G, Venet F, Pachot A, Lepape A. Monitoring immune dysfunctions in the septic patient: A new skin for the old ceremony. Mol Med 2008;14:64–78.
22. Fadlon E, Vordermeier S, Pearson TC, Mire-Sluis AR, Dumonde DC, Phillips J, Fishlock K, Brown KA. Blood polymorphonuclear leukocytes from the majority of sickle cell patients in the crisis phase of the disease show enhanced adhesion to vascular endothelium and increased expression of CD64. Blood 1998;91:266–274.
23. Icardi M, Erickson Y, Kilborn S, Stewart B, Grief B, Scharnweber G. CD64 index provides simple and predictive testing for detection and monitoring of sepsis and bacterial infection in hospital patients. J Clin Microbiol 2009;47:3914–3919.
24. Groselj-Grenc M, Ihan A, Pavcnik-Arnol M, Kopitar AN, Gmeiner-Stopar T, Derganc M. Neutrophil and monocyte CD64 indexes, lipopolysaccharide-binding protein, procalcitonin and C-reactive protein in sepsis of critically ill neonates and children. Intensive Care Med 2009;35:1950–1958.
25. Shipp MA, Stefano GB, Switzer SN, Griffin JD, Reinherz EL. CD10 (CALLA)/neutral endopeptidase 24.11 modulates inflammatory peptide-induced changes in neutrophil morphology, migration, and adhesion proteins and is itself regulated by neutrophil activation. Blood 1991;78:1834–1841.
26. Brown CC, Malech HL, Gallin JI. Intravenous endotoxin recruits a distinct subset of human neutrophils, defined by monoclonal antibody 31D8, from bone marrow to the peripheral circulation. Cell Immunol 1989;123:294–306.
27. Seligmann B, Malech HL, Melnick DA, Gallin JI. An antibody binding to human neutrophils demonstrates antigenic heterogeneity detected early in myeloid maturation which correlates with functional heterogeneity of mature neutrophils. J Immunol 1985;135:2647–2653.
28. Orr Y, Taylor JM, Bannon PG, Geczy C, Kritharides L. Circulating CD10-/CD16low neutrophils provide a quantitative index of active bone marrow neutrophil release. Br J Haematol 2005;131:508–519.
29. Angus DC. The search for effective therapy for sepsis: Back to the drawing board? JAMA 2011;306:2614–2615.