A novel quantitative assay of mitophagy: Combining high content fluorescence microscopy and mitochondrial DNA load to quantify mitophagy and identify novel pharmacological tools against pathogenic heteroplasmic mtDNA

Pharmacological Research

Volumn 100, Issue , 2015, Pages 24-35

  Diot, Alan ^a   Hinks Roberts, Alex ^a   Lodge, Tiffany ^a   Liao, Chunyan ^a   Dombi, Eszter ^a   Morten, Karl ^a   Brady, Stefen ^a   Fratter, Carl ^b   Carver, Janet ^a   Muir, Rebecca ^a   Davis, Ryan ^c   Green, Charlotte J ^a   Johnston, Iain ^d   Hilton Jones, David ^e   Sue, Carolyn ^c   Mortiboys, Heather ^f   Poulton, Joanna ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b CHURCHILL HOSPITAL   (United Kingdom)

^c UNIVERSITY OF SYDNEY   (Australia)

^d IMPERIAL COLLEGE LONDON   (United Kingdom)

^e JOHN RADCLIFFE HOSPITAL   (United Kingdom)

^f UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Chemical compounds studied in this article PubChem CID: Metformin 4091; PubChem CID: 3 MA 1673; PubChem CID: Bafilomycin A1 2287; PubChem CID: Chloroquine 2719; PubChem CID: Colchicine 6167; PubChem CID: E64d 65663; PubChem CID: Pepstatin A 16218527; PubChem CID: Phenanthroline 1318; PubChem CID: Rapamycin 5284616; PubChem CID: Trehalose 7427

Indexed keywords

GALACTOSE; HETEROPLASMIC MITOCHONDRIAL DNA; METFORMIN; MITOCHONDRIAL DNA; MITOCHONDRIAL PROTEIN; PROTEIN LC3 II; UNCLASSIFIED DRUG;

AEROBIC METABOLISM; ANALYTICAL EQUIPMENT; ARTICLE; AUTOPHAGOSOME; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA DETERMINATION; DRUG MECHANISM; DRUG SCREENING; ELECTRON MICROSCOPY; FLUORESCENCE MICROSCOPY; GENE MUTATION; GLYCOLYSIS; HETEROPLASMY; HUMAN; HUMAN CELL; MITOCHONDRIAL RESPIRATION; MITOPHAGY; PRIORITY JOURNAL; PROTEIN LOCALIZATION; QUANTITATIVE ANALYSIS; SKIN FIBROBLAST; ADOLESCENT; ADULT; AGED; AUTOPHAGY; BIOASSAY; DRUG EFFECTS; GENETICS; MIDDLE AGED; MITOCHONDRION; PHYSIOLOGY; PROCEDURES; VERY ELDERLY; YOUNG ADULT;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; AUTOPHAGY; BIOLOGICAL ASSAY; DNA, MITOCHONDRIAL; HUMANS; METFORMIN; MICROSCOPY, FLUORESCENCE; MIDDLE AGED; MITOCHONDRIA; MITOCHONDRIAL DEGRADATION; YOUNG ADULT;

EID: 84938631388     PISSN: 10436618     EISSN: 10961186     Source Type: Journal    
DOI: 10.1016/j.phrs.2015.07.014     Document Type: Article

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