Large-volume en-bloc staining for electron microscopy-based connectomics

Nature Communications

Volumn 6, Issue , 2015, Pages

  Hua, Yunfeng ^a   Laserstein, Philip ^a   Helmstaedter, Moritz ^a  

^a MAX PLANCK INSTITUTE FOR BRAIN RESEARCH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CORRELATION; ELECTRON MICROSCOPY; NEUROLOGY; RODENT; THREE-DIMENSIONAL MODELING; VOLUME;

ANIMAL EXPERIMENT; ARTICLE; BRAIN SIZE; CONNECTOME; CONTROLLED STUDY; ELECTRON MICROSCOPY; EMBEDDING; MALE; MOUSE; MYELINATED NERVE; NEOCORTEX; NONHUMAN; PERIOSTEUM; POSTSYNAPTIC DENSITY; SYNAPSE VESICLE; THREE DIMENSIONAL IMAGING; ANIMAL; GLIA; NERVE CELL; NEURITE; PROCEDURES; STAINING; ULTRASTRUCTURE;

ANIMALS; CONNECTOME; IMAGING, THREE-DIMENSIONAL; MICE; MICROSCOPY, ELECTRON; NEOCORTEX; NEURITES; NEUROGLIA; NEURONS; STAINING AND LABELING;

EID: 84938490941     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms8923     Document Type: Article

References (22)

1. Briggman, K. L. & Bock, D. D. Volume electron microscopy for neuronal circuit reconstruction. Curr. Opin. Neurobiol. 22, 154-161 (2012).
2. Mikula, S. & Denk, W. High-resolution whole-brain staining for electron microscopic circuit reconstruction. Nat. Methods 12, 541-546 (2015).
3. Helmstaedter, M. Cellular-resolution connectomics: challenges of dense neural circuit reconstruction. Nat. Methods 10, 501-507 (2013).
4. Palade, G. E. A study of fixation for electron microscopy. J. Exp. Med. 95, 285-298 (1952).
5. Watson, M. L. Staining of tissue sections for electron microscopy with heavy metals. J. Biophys. Biochem. Cytol. 4, 475-478 (1958).
6. Tapia, J. C. et al. High-contrast en bloc staining of neuronal tissue for field emission scanning electron microscopy. Nat. Protoc. 7, 193-206 (2012).
7. Wai, J. S. M. et al. A mechanistic insight leads to a greatly improved osmium-catalyzed asymmetric dihydroxylation process. J. Am. Chem. Soc. 111, 1123-1125 (1989).
8. Kwong, H.-L., Sorato, C., Ogino, Y., Chen, H. & Sharpless, K. B. Preclusion of the "second cycle" in the osmium-catalyzed asymmetric dihydroxylation of olefins leads to a superior process. Tetrahedron Lett. 31, 2999-3002 (1990).
9. Kolb, H. C., VanNieuwenhze, M. S. & Sharpless, K. B. Catalytic Asymmetric Dihydroxylation. Chem. Rev. 94, 2483-2547 (1994).
10. Locke, M., Krishnan, N. & McMahon, J. T. A routine method for obtaining high contrast without staining sections. J. Cell Biol. 50, 540-544 (1971).
11. Khan, A. A., Riemersma, J. C. & Booij, H. L. The reactions of osmium tetroxide with lipids and other compounds. J. Histochem. Cytochem. 9, 560-563 (1961).
12. Korn, E. D. A chromatographic and spectrophotometric study of the products of the reaction of osmium tetroxide with unsaturated lipids. J. Cell Biol. 34, 627-638 (1967).
13. White, D. L., Andrews, S. B., Faller, J. W. & Barrnett, R. J. The chemical nature of osmium tetroxide fixation and staining of membranes by x-ray photoelectron spectroscopy. Biochim. Biophys. Acta 436, 577-592 (1976).
14. Subbaraman, L. R., Subbaraman, J. & Behrman, E. J. Inorganic Chemistry (Department of Biochemistry, Ohio State Univ. 11, 2621-2627, 1972).
15. White, D. L., Mazurkiewicz, J. E. & Barrnett, R. J. A chemical mechanism for tissue staining by osmium tetroxide-ferrocyanide mixtures. J. Histochem. Cytochem. 27, 1084-1091 (1979).
16. Seligman, A. M., Wasserkrug, H. L. & Hanker, J. S. A new staining method (OTO) for enhancing contrast of lipid-containing membranes and droplets in osmium tetroxide-fixed tissue with osmiophilic thiocarbohydrazide(TCH). J. Cell Biol. 30, 424-432 (1966).
17. Willingham, M. C. & Rutherford, A. V. The use of osmiumthiocarbohydrazide-osmium (OTO) and ferrocyanide-reduced osmium methods to enhance membrane contrast and preservation in cultured cells. J. Histochem. Cytochem. 32, 455-460 (1984).
18. Briggman, K. L., Helmstaedter, M. & Denk, W. Wiring specificity in the direction-selectivity circuit of the retina. Nature 471, 183-188 (2011).
19. Holcomb, P. S. et al. Synaptic inputs compete during rapid formation of the calyx of Held: a new model system for neural development. J. Neurosci. 33, 12954-12969 (2013).
20. Seligman, A. M., Hanker, J. S., Wasserkrug, H., Dmochowski, H. & Katzoff, L. Histochemical demonstration of some oxidized macromolecules with thiocarbohydrazide (tch) or thiosemicarbazide (TSC) and osmium tetroxide. J. Histochem. Cytochem. 13, 629-639 (1965).
21. Helmstaedter, M., Briggman, K. L. & Denk, W. High-accuracy neurite reconstruction for high-throughput neuroanatomy. Nat. Neurosci. 14, 1081-1088 (2011).
22. Helmstaedter, M. et al. Connectomic reconstruction of the inner plexiform layer in the mouse retina. Nature 500, 168-174 (2013).