Successful use of saliva without DNA extraction for detection of macrolide-resistant Mycoplasma pneumoniae DNA in children using LNA probe-based real-time PCR

Journal of Infection and Chemotherapy

Volumn 19, Issue 6, 2013, Pages 1087-1092

  Komatsu, Haruki ^a,b   Tsunoda, Tomoyuki ^b   Inui, Ayano ^b   Sogo, Tsuyoshi ^b   Fujisawa, Tomoo ^b   Imura, Motoki ^a   Tateno, Akihiko ^a  

^a TOHO UNIVERSITY   (Japan)

^b Eastern Yokohama Hospital   (Japan)

Author keywords

Direct real time PCR; Locked nucleic acid; Macrolide resistant; Mycoplasma pneumoniae; Rapid diagnosis

Indexed keywords

MACROLIDE; RNA 23S;

ADOLESCENT; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; CHILD; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; DNA EXTRACTION; FEMALE; HUMAN; INTERMETHOD COMPARISON; LOCKED NUCLEIC ACID PROBE REAL TIME POLYMERASE CHAIN REACTION; MAJOR CLINICAL STUDY; MALE; MYCOPLASMA PNEUMONIA; MYCOPLASMA PNEUMONIAE; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; SALIVA ANALYSIS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; SPUTUM ANALYSIS;

ADOLESCENT; CHILD; CHILD, PRESCHOOL; DNA, BACTERIAL; FEMALE; HUMANS; INFANT; MALE; MYCOPLASMA PNEUMONIAE; OLIGONUCLEOTIDES; PNEUMONIA, MYCOPLASMA; REAL-TIME POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 23S; SALIVA;

EID: 84890858148     PISSN: 1341321X     EISSN: 14377780     Source Type: Journal    
DOI: 10.1007/s10156-013-0630-9     Document Type: Article

References (15)

1. Ieven M, Ursi D, Van Bever H, Quint W, Niesters HG, Goossens H. Detection of Mycoplasma pneumoniae by two polymerase chain reactions and role of M. pneumoniae in acute respiratory tract infections in pediatric patients. J Infect Dis. 1996;173(6):1445-52.
2. Welti M, Jaton K, Altwegg M, Sahli R, Wenger A, Bille J. Development of a multiplex real-time quantitative PCR assay to detect Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae in respiratory tract secretions. Diagn Microbiol Infect Dis. 2003;45(2):85-95.
3. Johnson MP, Haupt LM, Griffiths LR. Locked nucleic acid (LNA) single nucleotide polymorphism (SNP) genotype analysis and validation using real-time PCR. Nucleic Acids Res. 2004;32(6):e55.
4. Simeonov A, Nikiforov TT. Single nucleotide polymorphism genotyping using short, fluorescently labeled locked nucleic acid (LNA) probes and fluorescence polarization detection. Nucleic Acids Res. 2002;30(17):e91.
5. Vester B, Wengel J. LNA (locked nucleic acid): high-affinity targeting of complementary RNA and DNA. Biochemistry. 2004;43(42):13233-41.
6. Abele-Horn M, Busch U, Nitschko H, Jacobs E, Bax R, Pfaff F, et al. Molecular approaches to diagnosis of pulmonary diseases due to Mycoplasma pneumoniae. J Clin Microbiol. 1998;36(2):548-51.
7. Matsuoka M, Narita M, Okazaki N, Ohya H, Yamazaki T, Ouchi K, et al. Characterization and molecular analysis of macrolide-resistant Mycoplasma pneumoniae clinical isolates obtained in Japan. Antimicrob Agents Chemother. 2004;48(12):4624-30.
8. Suzuki N, Yuyama M, Maeda S, Ogawa H, Mashiko K, Kiyoura Y. Genotypic identification of presumptive Streptococcus pneumoniae by PCR using four genes highly specific for S. pneumoniae. J Med Microbiol. 2006;55(pt 6):709-14.
9. Morozumi M, Hasegawa K, Kobayashi R, Inoue N, Iwata S, Kuroki H, et al. Emergence of macrolide-resistant Mycoplasma pneumoniae with a 23S rRNA gene mutation. Antimicrob Agents Chemother. 2005;49(6):2302-6.
10. Morozumi M, Iwata S, Hasegawa K, Chiba N, Takayanagi R, Matsubara K, et al. Increased macrolide resistance of Mycoplasma pneumoniae in pediatric patients with community-acquired pneumonia. Antimicrob Agents Chemother. 2008;52(1):348-50.
11. Cao B, Zhao CJ, Yin YD, Zhao F, Song SF, Bai L, et al. High prevalence of macrolide resistance in Mycoplasma pneumoniae isolates from adult and adolescent patients with respiratory tract infection in China. Clin Infect Dis. 2010;51(2):189-94.
12. Dumke R, von Baum H, Luck PC, Jacobs E. Occurrence of macrolide-resistant Mycoplasma pneumoniae strains in Germany. Clin Microbiol Infect. 2010;16(6):613-6.
13. Chironna M, Sallustio A, Esposito S, Perulli M, Chinellato I, Di Bari C, et al. Emergence of macrolide-resistant strains during an outbreak of Mycoplasma pneumoniae infections in children. J Antimicrob Chemother. 2011;66(4):734-7.
14. Warshawsky I, Mularo F. Locked nucleic acid probes for enhanced detection of FLT3 D835/I836, JAK2 V617F and NPM1 mutations. J Clin Pathol. 2011;64(10):905-10.
15. Liu Y, Doring J, Hurek T. Bias in topoisomerase (TOPO)-cloning of multitemplate PCR products using locked nucleic acid (LNA)-substituted primers. J Microbiol Methods. 2012;91(3):483-6.