Determination of in-gel protein concentration by a ninhydrin-based method

Proteomics

Volumn 11, Issue 3, 2011, Pages 481-484

  Kang, Sung Ung ^a   Lubec, Gert ^a  

^a MEDICAL UNIVERSITY OF VIENNA   (Austria)

Author keywords

Hydrophobic protein; Ninhydrin assay; Protein quantification; Technology

Indexed keywords

NINHYDRIN; PROTEINASE;

ARTICLE; CHEMICAL REACTION; DIGESTION; HYDROLYSIS; HYDROPHILICITY; HYDROPHOBICITY; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN GEL BLOT ANALYSIS;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; GELS; HYDROLYSIS; INDICATORS AND REAGENTS; NINHYDRIN; PROTEINS;

EID: 79251508897     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.201000388     Document Type: Article

References (5)

1. Moore, S., Stein, W. H., Photometric ninhydrin method for use in the chromatography of amino acids. J. Biol. Chem. 1948, 176, 367-388.
2. Schwartz, T. B., Engel, F. L., A photometric ninhydrin method for the measurement of proteolysis. J. Biol. Chem. 1950, 184, 197-202.
3. Wessel, D., Flugge, U. I., A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Anal. Biochem. 1984, 138, 141-143.
4. Starcher, B., A ninhydrin-based assay to quantitate the total protein content of tissue samples. Anal. Biochem. 2001, 292, 125-129.
5. Friedman, M., Applications of the ninhydrin reaction for analysis of amino acids, peptides, and proteins to agricultural and biomedical sciences. J. Agric. Food Chem. 2004, 52, 385-406.