Directed evolution and mutagenesis of lysine decarboxylase from Hafnia alvei AS1.1009 to improve its activity toward efficient cadaverine production

Biotechnology and Bioprocess Engineering

Volumn 20, Issue 3, 2015, Pages 439-446

  Wang, Chen ^a   Zhang, Kai ^a   Zhongjun, Chen ^b   Cai, Heng ^a   Honggui, Wan ^a   Ouyang, Pingkai ^a  

^a NANJING TECH UNIVERSITY   (China)

^b INNER MONGOLIA AGRICULTURAL UNIVERSITY   (China)

Author keywords

cadaverine; directed evolution; DNA shuffling; error prone PCR; lysine decarboxylase

Indexed keywords

AMINO ACIDS; ESCHERICHIA COLI; HYDROPHOBIC CHROMATOGRAPHY; MUTAGENESIS; POLYMERASE CHAIN REACTION; PURIFICATION;

CADAVERINE; DECARBOXYLASES; DIRECTED EVOLUTION; DNA SHUFFLING; ERROR PRONE PCR;

CATALYST ACTIVITY;

CADAVERINE; LYSINE; LYSINE DECARBOXYLASE;

ARTICLE; DNA SHUFFLING; ENZYME KINETICS; ENZYME MECHANISM; ENZYME PURIFICATION; HAFNIA ALVEI; HIGH THROUGHPUT SCREENING; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; MOLECULAR EVOLUTION; MUTANT; NONHUMAN; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS;

HAFNIA ALVEI;

EID: 84937911782     PISSN: 12268372     EISSN: 19763816     Source Type: Journal    
DOI: 10.1007/s12257-014-0690-4     Document Type: Article

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