Random mutagenesis and recombination of sam1 gene by integrating error-prone PCR with staggered extension process

Biotechnology Letters

Volumn 30, Issue 7, 2008, Pages 1227-1232

  An, Yingfeng ^a,b   Ji, Jianfei ^c   Wu, Wenfang ^a   Huang, Ribo ^d   Wei, Yutuo ^d   Xiu, Zhilong ^e  

^a INSTITUTE OF APPLIED ECOLOGY   (China)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (France)

^c CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

^d GUANGXI UNIVERSITY   (China)

^e DALIAN UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

AdoMet synthetase; Directed evolution; DNA recombination; Error prone PCR; S adenosylmethionine

Indexed keywords

ADOMET SYNTHETASE; DIRECTED EVOLUTION; DNA RECOMBINATION; ERROR-PRONE PCR; S-ADENOSYLMETHIONINE;

DNA; ERROR ANALYSIS; ETHANOL; GENES;

RANDOM PROCESSES;

ANTIMETABOLITE; ETHIONINE; METHIONINE ADENOSYLTRANSFERASE; S ADENOSYLMETHIONINE; SACCHAROMYCES CEREVISIAE PROTEIN;

ARTICLE; BIOSYNTHESIS; DIRECTED MOLECULAR EVOLUTION; DRUG ANTAGONISM; DRUG EFFECT; ENZYMOLOGY; GENETIC RECOMBINATION; GENETICS; METHODOLOGY; MUTAGENESIS; MUTATION; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE;

ANTIMETABOLITES; DIRECTED MOLECULAR EVOLUTION; ETHIONINE; METHIONINE ADENOSYLTRANSFERASE; MUTAGENESIS; MUTATION; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; S-ADENOSYLMETHIONINE; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 44949211457     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9674-9     Document Type: Article

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