Using solutes and kinetics to probe large conformational changes in the steps of transcription initiation

Methods in Molecular Biology

Volumn 1276, Issue , 2015, Pages 241-261

  Ruff, Emily F ^a   Kontur, Wayne S ^a   Thomas Record, M ^a  

^a NONE

Author keywords

Conformational changes; Kinetics; Mechanism; Open complex formation; RNA polymerase; Solute effects

Indexed keywords

DNA FRAGMENT; HEPARIN; PYROXYLIN; RNA POLYMERASE; UREA; DNA DIRECTED RNA POLYMERASE; MOLECULAR PROBE;

ARTICLE; BINDING ASSAY; BINDING KINETICS; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; DISSOCIATION; ENTEROBACTERIA PHAGE T7; EQUILIBRIUM CONSTANT; ESCHERICHIA COLI; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN FOLDING; SOLUTE; SURFACE PROPERTY; TRANSCRIPTION INITIATION; ENZYMOLOGY; KINETICS; METABOLISM; MOLECULAR PROBE; PHYSIOLOGY; PROTEIN CONFORMATION;

ESCHERICHIA COLI;

COLLODION; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; KINETICS; MOLECULAR PROBES; PROTEIN CONFORMATION; TRANSCRIPTION INITIATION, GENETIC;

EID: 84937818044     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2392-2_14     Document Type: Article

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