Alanine-Scanning Mutational Analysis of Durancin GL Reveals Residues Important for Its Antimicrobial Activity

Journal of Agricultural and Food Chemistry

Volumn 63, Issue 28, 2015, Pages 6402-6409

  Ju, Xingrong ^a   Chen, Xinquan ^a   Du, Lihui ^a   Wu, Xueyou ^a   Liu, Fang ^b   Yuan, Jian ^a  

^a NANJING UNIVERSITY OF FINANCE AND ECONOMICS   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

alanine scanning mutation; antilisteria; durancin GL; Enterococcus

Indexed keywords

AMINO ACIDS; LISTERIA; SCANNING; SPECTRUM ANALYSIS;

ALANINE-SCANNING MUTATION; ANTI-MICROBIAL ACTIVITY; ANTILISTERIA; ANTIMICROBIAL SPECTRA; DURANCIN GL; ENTEROCOCCUS; LISTERIA MONOCYTOGENES; SITE DIRECTED MUTAGENESIS;

MICROORGANISMS;

ENTEROCOCCUS; ENTEROCOCCUS DURANS; LISTERIA MONOCYTOGENES;

ALANINE; ANTIINFECTIVE AGENT; BACTERIOCIN; DURANCIN GL, ENTEROCOCCUS DURANS; HYBRID PROTEIN;

AMINO ACID SEQUENCE; CHEMISTRY; DRUG EFFECTS; ENTEROCOCCUS; GENE EXPRESSION; GENETICS; LISTERIA MONOCYTOGENES; METABOLISM; NUCLEOTIDE SEQUENCE; STRUCTURE ACTIVITY RELATION;

ALANINE; AMINO ACID SEQUENCE; ANTI-INFECTIVE AGENTS; BACTERIOCINS; DNA MUTATIONAL ANALYSIS; ENTEROCOCCUS; GENE EXPRESSION; LISTERIA MONOCYTOGENES; RECOMBINANT FUSION PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 84937691045     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/acs.jafc.5b02114     Document Type: Article

References (28)

1. Drider, D.; Fimland, G.; Héchard, Y.; McMullen, L. M.; Prévost, H. The continuing story of class IIa bacteriocins Microbiol. Mol. Biol. Rev. 2006, 70, 564-582 10.1128/MMBR.00016-05
2. Klaenhammer, T. R. Genetics of bacteriocins produced by lactic acid bacteria FEMS Microbiol. Rev. 1993, 12, 39-85 10.1016/0168-6445(93)90057-G
3. Fimland, G.; Johnsen, L.; Dalhus, B.; Nissen-Meyer, J. Pediocin-like antimicrobial peptides (class IIa bacteriocins) and their immunity proteins: biosynthesis, structure, and mode of action J. Pept. Sci. 2005, 11, 688-696 10.1002/psc.699
4. Cleveland, J.; Montville, T. J.; Nes, I. F.; Chikindas, M. L. Bacteriocins: safe, natural antimicrobials for food preservation Int. J. Food Microbiol. 2001, 71, 1-20 10.1016/S0168-1605(01)00560-8
5. Cotter, P. D.; Hill, C.; Ross, R. P. Bacteriocins: developing innate immunity for food Nat. Rev. Microbiol. 2005, 3, 777-788 10.1038/nrmicro1273
6. Corr, S. C.; Li, Y.; Riedel, C. U.; O'Toole, P. W.; Hill, C.; Gahan, C. G. Bacteriocin production as a mechanism for the antiinfective activity of Lactobacillus salivarius UCC118 Proc. Natl. Acad. Sci. U. S. A. 2007, 104, 7617-7621 10.1073/pnas.0700440104
7. Lohans, C. T.; Vederas, J. C. Development of class IIa bacteriocins as therapeutic agents Int. J. Microbiol. 2012, 2012, 1-13 10.1155/2012/386410
8. Cui, Y.; Zhang, C.; Wang, Y.; Shi, J.; Zhang, L.; Ding, Z.; Qu, X.; Cui, H. Class IIa bacteriocins: Diversity and new developments Int. J. Mol. Sci. 2012, 13, 16668-16707 10.3390/ijms131216668
9. Fimland, G.; Blingsmo, O. R.; Sletten, K.; Jung, G.; Nes, I. F.; Nissen-Meyer, J. New biologically active hybrid bacteriocins constructed by combining regions from various pediocin-like bacteriocins: the C-terminal region is important for determining specificity Appl. Environ. Microbiol. 1996, 62, 3313-3318
10. Miller, K. W.; Schamber, R.; Osmanagaoglu, O.; Ray, B. Isolation and characterization of pediocin AcH chimeric protein mutants with altered bactericidal activity Appl. Environ. Microbiol. 1998, 64, 1997-2005
11. Fimland, G.; Eijsink, V. G.; Nissen-Meyer, J. Mutational analysis of the role of tryptophan residues in an antimicrobial peptide Biochemistry 2002, 41, 9508-9515 10.1021/bi025856q
12. Kazazic, M.; Nissen-Meyer, J.; Fimland, G. Mutational analysis of the role of charged residues in target-cell binding, potency and specificity of the pediocin-like bacteriocin sakacin P Microbiology 2002, 148, 2019-2027
13. Morisset, D.; Berjeaud, J. M.; Marion, D.; Lacombe, C.; Frère, J. Mutational analysis of mesentericin Y105, an anti-Listeria bacteriocin, for determination of impact on bactericidal activity, in vitro secondary structure, and membrane interaction Appl. Environ. Microbiol. 2004, 70, 4672-4680 10.1128/AEM.70.8.4672-4680.2004
14. Quadri, L. E.; Yan, L. Z.; Stiles, M. E.; Vederas, J. C. Effect of amino acid substitutions on the activity of carnobacteriocin B2 Overproduction of the antimicrobial peptide, its engineered variants, and its precursor in Escherichia coli J. Biol. Chem. 1997, 272, 3384-3388 10.1074/jbc.272.6.3384
15. Tominaga, T.; Hatakeyama, Y. Determination of essential and variable residues in pediocin PA-1 by NNK scanning Appl. Environ. Microbiol. 2006, 72, 1141-1147 10.1128/AEM.72.2.1141-1147.2006
16. Du, L.; Somkuti, G. A.; Renye, J. A., Jr. Molecular analysis of the bacteriocin-encoding plasmid pDGL1 from Enterococcus durans and genetic characterization of the Durancin GL locus Microbiology 2012, 158, 1523-1532 10.1099/mic.0.055624-0
17. Du, L.; Somkuti, G. A.; Renye, J. A., Jr.; Huo, G. Properties of Durancin GL, a new antilisterial bacteriocin produced by Enterococcus durans 41D J. Food Saf. 2012, 32, 74-83 10.1111/j.1745-4565.2011.00346.x
18. Sambrook, J.; Fritsch, E. F.; Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 1989a.
19. Sambrook, J.; Fritsch, E.; Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 1989b.
20. Pilet, M. F.; Dousset, X.; Barré, R.; Novel, G.; Desmazeaud, M.; Piard, J. C. Evidence for two bacteriocins produced by Carnobacterium piscicola and Carnobacterium divergens isolated from fish and active against Listeria monocytogenes J. Food Protect. 1995, 58, 256-262
21. Nissen-Meyer, J.; Rogne, P.; Oppegard, C.; Haugen, H.; Kristiansen, P. Structure-function relationships of the non-lanthionine-containing peptide (class II) bacteriocins produced by gram-positive bacteria Curr. Pharm. Biotechnol. 2009, 10, 19-37 10.2174/138920109787048661
22. Eijsink, V. G.; Skeie, M.; Middelhoven, P. H.; Brurberg, M. B.; Nes, I. F. Comparative studies of class IIa bacteriocins of lactic acid bacteria Appl. Environ. Microbiol. 1998, 64, 3275-3281
23. Chen, Y.; Shapira, R.; Eisenstein, M.; Montville, T. J. Functional characterization of pediocin PA-1 binding to liposomes in the absence of a protein receptor and its relationship to a predicted tertiary structure Appl. Environ. Microbiol. 1997, 63, 524-531
24. Fimland, G.; Pirneskoski, J.; Kaewsrichan, J.; Jutila, A.; Kristiansen, P. E.; Kinnunen, P. K.; Nissen-Meyer, J. Mutational analysis and membrane-interactions of the β-sheet-like N-terminal domain of the pediocin-like antimicrobial peptide sakacin P Biochim. Biophys. Acta, Proteins Proteomics 2006, 1764, 1132-1140 10.1016/j.bbapap.2006.04.007
25. Haugen, H. S.; Fimland, G.; Nissen-Meyer, J. Mutational analysis of residues in the helical region of the class IIa bacteriocin pediocin PA-1 Appl. Environ. Microbiol. 2011, 77 (6) 1966-1972 10.1128/AEM.02488-10
26. Hu, W.; Lazo, N.; Cross, T. Tryptophan dynamics and structural refinement in a lipid bilayer environment: solid state NMR of the gramicidin channel Biochemistry 1995, 34, 14138-14146 10.1021/bi00043a019
27. Hu, W.; Cross, T. Tryptophan hydrogen bonding and electric dipole moments: functional roles in the gramicidin channel and implications for membrane proteins Biochemistry 1995, 34, 14147-14155 10.1021/bi00043a020
28. Richard, C.; Canon, R.; Naghmouchi, K.; Bertrand, D.; Prevost, H.; Drider, D. Evidence on correlation between number of disulfide bridge and toxicity of class IIa bacteriocins Food Microbiol. 2006, 23, 175-183 10.1016/j.fm.2005.02.001