Isolation and characterization of pediocin AcH chimeric protein mutants with altered bactericidal activity

Applied and Environmental Microbiology

Volumn 64, Issue 6, 1998, Pages 1997-2005

  Miller, Kurt W ^a   Schamber, Robin ^a   Osmanagaoglu, Ozlem ^a,b   Ray, Bibek ^a  

^a UNIVERSITY OF WYOMING   (United States)

^b MIDDLE EAST TECHNICAL UNIVERSITY   (Turkey)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; CHIMERIC PROTEIN; DNA; MALTOSE BINDING PROTEIN; PEDIOCIN;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERICIDAL ACTIVITY; ENTEROCOCCUS FAECALIS; ESCHERICHIA COLI; LACTOBACILLUS PLANTARUM; LEUCONOSTOC; LISTERIA; MUTAGENESIS; MUTANT; NONHUMAN; PEDIOCOCCUS; PLASMID; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; PROTEIN ISOLATION; STRUCTURAL GENE;

ACRONICTA LEPORINA; ENTEROCOCCUS FAECALIS; ESCHERICHIA COLI; LACTOBACILLUS PLANTARUM; LEUCONOSTOC; LEUCONOSTOC MESENTEROIDES; LISTERIA INNOCUA; PEDIOCOCCUS; PEDIOCOCCUS ACIDILACTICI; STREPTOCOCCUS;

EID: 0031745937     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.6.1997-2005.1998     Document Type: Article

References (36)

1. Amann, E., J. Brosius, and M. Ptashne. 1983. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25:167-178.
2. Bhunia, A. K., M. C. Johnson, B. Ray, and N. Kalchayanand. 1991. Mode of action of pediocin AcH from Pediococcus acidilactici H on sensitive bacterial strains. J. Appl. Bacteriol. 70:25-30.
3. Biswas, S. R., P. Ray, M. C. Johnson, and B. Ray. 1991. Influence of growth conditions on the production of bacteriocin, pediocin AcH, by Pediococcus acidilactici H. Appl. Environ. Microbiol. 57:1265-1267.
4. Bukhtiyarova, M., R. Yang, and B. Ray. 1994. Analysis of the pediocin AcH gene cluster from plasmid pSMB74 and its expression in a pediocin-negative Pediococcus acidilactici strain. Appl. Environ. Microbiol. 60:3405-3408.
5. Chen, Y., R. Shapira, M. Eisenstein, and T. J. Montville. 1997. Functional characterization of pediocin PA-1 binding to liposomes in the absence of a protein receptor and its relationship to a predicted tertiary structure. Appl. Environ. Microbiol. 63:524-531.
6. Chen, Y., R. D. Ludescher, and T. J. Montville. 1997. Electrostatic interactions, but not the YGNGV consensus motif, govern the binding of pediocin PA-1 and its fragments to phospholipid vesicles. Appl. Environ. Microbiol. 63:4770-4777.
7. Chikindas, M. L., M. J. Garcia-Garcera, A. J. M. Driessen, A. M. Ledeboer, J. Nissen-Meyer, I. F. Nes, T. Abee, W. N. Konings, and G. Venema. 1993. Pediocin PA-1, a bacteriocin from Pediococcus acidilactici PAC 1.0, forms hydrophilic pores in the cytoplasmic membrane of target cells. Appl. Environ. Microbiol. 59:3577-3584.
8. Cowan, S. W., and J. P. Rosenbusch. 1994. Folding pattern diversity of integral membrane proteins. Science 264:914-916.
9. Eisenberg, D., E. Schwarz, M. Komaroray, and R. Wall. 1984. Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J. Mol. Biol. 179:125-142.
10. Engelman, D. M., and T. A. Steitz. 1981. The spontaneous insertion of proteins into and across membranes: the helical hairpin hypothesis. Cell 23:79-88.
11. Fath, M. J., and R. Kolter. 1993. ABC-transporters: bacterial exporters. Microbiol. Rev. 57:995-1017.
12. Fimland, G., O. R. Blingsmo, K. Sletten, G. Jung, I. F. Nes, and J. Nissen-Meyer. 1996. New biologically active hybrid bacteriocins constructed by combining regions from various pediocin-like bacteriocins: the C-terminal region is important for determining specificity. Appl. Environ. Microbiol. 62:3313-3318.
13. Hastings, J. W., M. Sailer, K. Johnson, K. L. Roy, J. C. Vederas, and M. E. Stiles. 1991. Characterization of leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidum. J. Bacteriol. 173:7491-7500.
14. Henderson, J. T., A. L. Chopko, and P. D. van Wassenaar. 1992. Purification and primary structure of pediocin PA-1 produced by Pediococcus acidilactici PAC-1.0. Arch. Biochem. Biophys. 295:5-12.
15. Higuchi, R. 1990. Recombinant PCR, p. 177-183. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, and T. J. White (ed.), PCR protocols. A guide to methods and applications. Academic Press, Inc., San Diego, Calif.
16. Holck, A., L. Axelsson, S.-E. Birkeland, T. Aukrust, and H. Bloom. 1992. Purification and amino acid sequence of sakacin A, a bacteriocin from Lactobacillus sake LB706. J. Gen. Microbiol. 138:2715-2720.
17. Holck, A., L. Axelsson, K. Huhne, and L. Krockel. 1994. Purification and cloning of sakacin 674, a bacteriocin from Lactobacillus sake LB674. FEMS Microbiol. Lett. 115:143-150.
18. Hutchinson, E. G., and J. M. Thornton. 1994. A revised set of potentials for β-turn formation in proteins. Protein Sci. 3:2207-2216.
19. Jack, R. W., J. R. Tagg, and B. Ray. 1995. Bacteriocins of gram-positive bacteria. Microbiol. Rev. 59:171-200.
20. Kaiser, E. T., and F. J. Kezdy. 1984. Amphiphilic secondary structure: design of peptide hormones. Science 223:249-255.
21. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
22. Leung, D. W., E. Chen, and D. V. Goeddel. 1989. A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction. Technique 1:11-15.
23. Liu, L.-P., and C. M. Deber. 1997. Anionic phospholipids modulate peptide insertion into membranes. Biochemistry 36:5476-5482.
24. Marugg, J. D., C. F. Gonzalez, B. S. Kunka, A. M. Ledeboer, M. J. Pucci, M. Y. Toonen, S. A. Walker, L. C. M. Zoetmulder, and P. A. Vandenbergh. 1992. Cloning, expression, and nucleotide sequence of genes involved in production of pediocin PA-1, a bacteriocin from Pediococcus acidilactici PAC1.0. Appl. Environ. Microbiol. 58:2360-2367.
25. Miller, K. W., R. Schamber, Y. Chen, and B. Ray. 1998. Production of active chimeric pediocin AcH in Escherichia coli in the absence of processing and secretion genes from the Pediococcus pap operon. Appl. Environ. Microbiol. 64:14-20.
26. Motlagh, A. M., M. Bukhtiyarova, and B. Ray. 1994. Complete nucleotide sequence of pSMB74, a plasmid encoding the production of pediocin AcH in Pediococcus acidilactici. Lett. Appl. Microbiol. 18:305-312.
27. Ray, B. 1996. Characteristics and application of pediocin(s) of Pediococcus acidilactici: pediocin PA-1/AcH, p. 155-203. In T. F. Bozoglu and B. Ray (ed.), Lactic acid bacteria: current advances in metabolism, genetics, and applications. Springer, New York, N.Y.
28. 13C-labeled media from Anabaena sp. for universal isotopic labeling of bacteriocins: NMR resonance assignments of leucocin A from Leuconostoc gelidum and nisin A from Lactococcus lactis. Biochemistry 32:310-318.
29. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
30. Taylor, J. W., J. Ott, and F. Eckstein. 1985. The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA. Nucleic Acids Res. 13:8765-8785.
31. Tichaczek, P. S., R. F. Vogel, and W. P. Hammes. 1993. Cloning and sequencing of curA encoding curvacin A, the bacteriocin produced by Lactobacillus curvatus LTH1174. Arch. Microbiol. 160:279-283.
32. Tichaczek, P. S., R. F. Vogel, and W. Hammes. 1994. Cloning and sequencing sakP encoding sakacin P, the bacteriocin produced by Lactobacillus sake LTH673. Microbiology 140:361-367.
33. Venema, K., J. Kok, J. D. Marugg, M. Y. Toonen, A. M. Ledeboer, G. Venema, and M. L. Chikindas. 1995. Functional analysis of the pediocin operon of Pediococcus acidilactici PAC 1.0: PedB is the immunity protein and PedD is the precursor processing enzyme. Mol. Microbiol. 17:515-522.
34. Wieprecht, T., M. Dathe, M. Beyermann, E. Krause, W. L. Maloy, D. L. MacDonald, and M. Bienert. 1997. Peptide hydrophobicity controls the activity and selectivity of magainin 2 amide in interaction with membranes. Biochemistry 36:6124-6132.
35. Yang, R., M. C. Johnson, and B. Ray. 1992. Novel method to extract large amounts of bacteriocins from lactic acid bacteria. Appl. Environ. Microbiol. 58:3355-3359.
36. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.