Preparation of single-cell RNA-Seq libraries for next generation sequencing

Current Protocols in Molecular Biology

Volumn 2014, Issue , 2014, Pages 4.22.1-4.22.17

  Trombetta, John J ^a   Gennert, David ^a   Lu, Diana ^a   Satija, Rahul ^a   Shalek, Alex K ^b   Regev, Aviv ^a,c  

^a BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^b HARVARD UNIVERSITY   (United States)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Cell type; Low input RNA Seq; Next generation sequencing; Single cell; Single cell RNA Seq; Smart seq; Smart seq2; Template switching; Transcriptomics

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA; RNA DIRECTED DNA POLYMERASE; TRANSCRIPTOME;

ARTICLE; CLINICAL PROTOCOL; CONTROLLED STUDY; ENZYME ACTIVITY; GENE AMPLIFICATION; GENE EXPRESSION; HIGH THROUGHPUT SEQUENCING; MOLECULAR LIBRARY; NEXT GENERATION SEQUENCING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION; RNA ANALYSIS; RNA SEQUENCE; SINGLE CELL RNA SEQUENCE; ANIMAL; CHEMISTRY; GENE LIBRARY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; PROCEDURES; SEQUENCE ANALYSIS;

ANIMALS; DNA, COMPLEMENTARY; GENE LIBRARY; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; RNA, MESSENGER; SEQUENCE ANALYSIS, RNA;

EID: 84937535711     PISSN: 19343639     EISSN: 19343647     Source Type: Journal    
DOI: 10.1002/0471142727.mb0422s107     Document Type: Article

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