CDC42 Gtpase Activation Affects Hela Cell DNA Repair and Proliferation Following UV Radiation-Induced Genotoxic Stress

Journal of Cellular Biochemistry

Volumn 116, Issue 9, 2015, Pages 2086-2097

  Ascer, Liv G ^a   Magalhaes, Yuli T ^a   Espinha, Gisele ^a   Osaki, Juliana H ^a   Souza, Renan C ^a   Forti, Fabio L ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

CDC42 GTPASE; CONSTITUTIVELY ACTIVE CDC42 V12 MUTANT; DNA DAMAGE AND REPAIR; MIGRATION; PROLIFERATION AND CELL CYCLE; ULTRAVIOLET RADIATION

Indexed keywords

CHECKPOINT KINASE 1; GUANOSINE TRIPHOSPHATASE; HISTONE H2AX; PROTEIN CDC42; RHO GUANINE NUCLEOTIDE BINDING PROTEIN; TUBULIN;

AGING; ARTICLE; CELL ADHESION; CELL CYCLE PROGRESSION; CELL MIGRATION; CELL POLARITY; CELL PROLIFERATION; CELL SURVIVAL; CONTROLLED STUDY; DNA REPAIR; ENZYME ACTIVATION; G2 PHASE CELL CYCLE CHECKPOINT; GENOTOXICITY; HELA CELL LINE; HUMAN; HUMAN CELL; M PHASE CELL CYCLE CHECKPOINT; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RADIOSENSITIVITY; SIGNAL TRANSDUCTION; TRANSCRIPTION REGULATION; ULTRAVIOLET RADIATION; WILD TYPE; ADVERSE EFFECTS; CELL CYCLE CHECKPOINT; CELL MOTION; GENETICS; METABOLISM; MUTATION; RADIATION RESPONSE; RADIATION TOLERANCE;

CDC42 GTP-BINDING PROTEIN; CELL CYCLE CHECKPOINTS; CELL MOVEMENT; CELL PROLIFERATION; CELL SURVIVAL; DNA REPAIR; HELA CELLS; HUMANS; MUTATION; RADIATION TOLERANCE; ULTRAVIOLET RAYS;

EID: 84937460080     PISSN: 07302312     EISSN: 10974644     Source Type: Journal    
DOI: 10.1002/jcb.25166     Document Type: Article

References (46)

1. Bartek J, Lukas J,. 2001. Mammalian G1- and S-phase checkpoints in response to DNA damage. Curr Opin Cell Biol 13: 738-747.
2. Bray K, Brakebusch C, Vargo-Gogola T,. 2011. The Rho GTPase Cdc42 is required for primary mammary epithelial cell morphogenesis in vitro. Small GTPases 2: 247-258.
3. Cerione RA,. 2004. Cdc42: New roads to travel. Trends Cell Biol 14: 127-132.
4. Chen F, Ma L, Parrini MC, Mao X, Lopez M, Wu C, Marks PW, Davidson L, Kwiatkowski DJ, Kirchhausen T, Orkin SH, Rosen FS, Mayer BJ, Kirschner MW, Alt FW,. 2000. Cdc42 is required for PIP(2)-induced actin polymerization and early development but not for cell viability. Curr Biol 10: 758-765.
5. Chen QY, Jiao DM, Yao QH, Yan J, Song J, Chen FY, Lu GH, Zhou JY,. 2012. Expression analysis of Cdc42 in lung cancer and modulation of its expression by curcumin in lung cancer cell lines. Int J Oncol 40: 1561-1568.
6. Cho KA, Ryu SJ, Oh YS, Park JH, Lee JW, Kim HP, Kim KT, Jang IS, Park SC,. 2004. Morphological adjustment of senescent cells by modulating caveolin-1 status. J Biol Chem 279: 42270-42278.
7. Deevi R, Fatehullah A, Jagan I, Nagaraju M, Bingham V, Campbell FC,. 2011. PTEN regulates colorectal epithelial apoptosis through Cdc42 signalling. Br J Cancer 105: 1313-1321.
8. Evans T, Brown ML, Fraser ED, Northup JK,. 1986. Purification of the major GTP-binding proteins from human placental membranes. J Biol Chem 261: 7052-7059.
9. Florian MC, Dorr K, Niebel A, Daria D, Schrezenmeier H, Rojewski M, Filippi MD, Hasenberg A, Gunzer M, Scharffetter-Kochanek K, Zheng Y, Geiger H,. 2012. Cdc42 activity regulates hematopoietic stem cell aging and rejuvenation. Cell Stem Cell 10: 520-530.
10. Forti FL, Armelin HA,. 2007. Vasopressin triggers senescence in K-ras transformed cells via RhoA-dependent downregulation of cyclin D1. Endocr Relat Cancer 14: 1117-1125.
11. Freitas AA, de Magalhaes JP,. 2011. A review and appraisal of the DNA damage theory of ageing. Mutat Res 728: 12-22.
12. Friesland A, Zhao Y, Chen YH, Wang L, Zhou H, Lu Q,. 2013. Small molecule targeting Cdc42-intersectin interaction disrupts Golgi organization and suppresses cell motility. Proc Natl Acad Sci USA 110: 1261-1266.
13. Gadea G, Lapasset L, Gauthier-Rouviere C, Roux P,. 2002. Regulation of Cdc42-mediated morphological effects: A novel function for p53. EMBO J 21: 2373-2382.
14. Gadea G, Sanz-Moreno V, Self A, Godi A, Marshall CJ,. 2008. DOCK10-mediated Cdc42 activation is necessary for amoeboid invasion of melanoma cells. Curr Biol 18: 1456-1465.
15. Gjoerup O, Lukas J, Bartek J, Willumsen BM,. 1998. Rac and Cdc42 are potent stimulators of E2F-dependent transcription capable of promoting retinoblastoma susceptibility gene product hyperphosphorylation. J Biol Chem 273: 18812-18818.
16. Heasman SJ, Ridley AJ,. 2008. Mammalian Rho GTPases: New insights into their functions from in vivo studies. Nature reviews. Mol Cell Biol 9: 690-701.
17. Heynen SR, Tanimoto N, Joly S, Seeliger MW, Samardzija M, Grimm C,. 2011. Retinal degeneration modulates intracellular localization of CDC42 in photoreceptors. Mol Vis 17: 2934-2946.
18. Hong L, Kenney SR, Phillips GK, Simpson D, Schroeder CE, Noth J, Romero E, Swanson S, Waller A, Strouse JJ, Carter M, Chigaev A, Ursu O, Oprea T, Hjelle B, Golden JE, Aube J, Hudson LG, Buranda T, Sklar LA, Wandinger-Ness A,. 2013. Characterization of a Cdc42 protein inhibitor and its use as a molecular probe. J Biol Chem 288: 8531-8543.
19. Johnson DI,. 1999. Cdc42: An essential Rho-type GTPase controlling eukaryotic cell polarity. Microbiol Mol Biol Rev 63: 54-105.
20. Johnson DI, Pringle JR,. 1990. Molecular characterization of CDC42, a Saccharomyces cerevisiae gene involved in the development of cell polarity. J Cell Biol 111: 143-152.
21. Kaibuchi K, Kuroda S, Amano M,. 1999. Regulation of the cytoskeleton and cell adhesion by the Rho family GTPases in mammalian cells. Annu Rev Biochem 68: 459-486.
22. Kamai T, Yamanishi T, Shirataki H, Takagi K, Asami H, Ito Y, Yoshida K,. 2004. Overexpression of RhoA, Rac1, and Cdc42 GTPases is associated with progression in testicular cancer. Clin Cancer Res 10: 4799-4805.
23. Kerber RA, O'Brien E, Cawthon RM,. 2009. Gene expression profiles associated with aging and mortality in humans. Aging Cell 8: 239-250.
24. Liang CC, Park AY, Guan JL,. 2007. In vitro scratch assay: A convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2: 329-333.
25. Lin Q, Yang W, Cerione RA,. 2006. Measurement of epidermal growth factor receptor turnover and effects of Cdc42. Methods Enzymol 406: 614-625.
26. Melendez J, Grogg M, Zheng Y,. 2011. Signaling role of Cdc42 in regulating mammalian physiology. J Biol Chem 286: 2375-2381.
27. Molnar A, Theodoras AM, Zon LI, Kyriakis JM,. 1997. Cdc42Hs, but not Rac1, inhibits serum-stimulated cell cycle progression at G1/S through a mechanism requiring p38/RK. J Biol Chem 272: 13229-13235.
28. Munemitsu S, Innis MA, Clark R, McCormick F, Ullrich A, Polakis P,. 1990. Molecular cloning and expression of a G25K cDNA, the human homolog of the yeast cell cycle gene CDC42. Mol Cell Biol 10: 5977-5982.
29. Muris DF, Verschoor T, Divecha N, Michalides RJ,. 2002. Constitutive active GTPases Rac and Cdc42 are associated with endoreplication in PAE cells. Eur J Cancer 38: 1775-1782.
30. Na S, Li B, Grewal IS, Enslen H, Davis RJ, Hanke JH, Flavell RA,. 1999. Expression of activated CDC42 induces T cell apoptosis in thymus and peripheral lymph organs via different pathways. Oncogene 18: 7966-7974.
31. Nobes CD, Hall A,. 1995. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell 81: 53-62.
32. Palomera-Sanchez Z, Zurita M,. 2011. Open, repair and close again: Chromatin dynamics and the response to UV-induced DNA damage. DNA Repair 10: 119-125.
33. Philips A, Roux P, Coulon V, Bellanger JM, Vie A, Vignais ML, Blanchard JM,. 2000. Differential effect of Rac and Cdc42 on p38 kinase activity and cell cycle progression of nonadherent primary mouse fibroblasts. J Biol Chem 275: 5911-5917.
34. Ponimaskin E, Voyno-Yasenetskaya T, Richter DW, Schachner M, Dityatev A,. 2007. Morphogenic signaling in neurons via neurotransmitter receptors and small GTPases. Mol Neurobiol 35: 278-287.
35. Rathinam R, Berrier A, Alahari SK,. 2011. Role of Rho GTPases and their regulators in cancer progression. Front Biosci 16: 2561-2571.
36. Ren XD, Kiosses WB, Schwartz MA,. 1999. Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton. EMBO J 18: 578-585.
37. Seo M, Cho CH, Lee YI, Shin EY, Park D, Bae CD, Lee JW, Lee ES, Juhnn YS,. 2004. Cdc42-dependent mediation of UV-induced p38 activation by G protein betagamma subunits. J Biol Chem 279: 17366-17375.
38. Shinjo K, Koland JG, Hart MJ, Narasimhan V, Johnson DI, Evans T, Cerione RA,. 1990. Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42. Proc Natl Acad Sci USA 87: 9853-9857.
39. Stengel K, Zheng Y,. 2011. Cdc42 in oncogenic transformation, invasion, and tumorigenesis. Cell Signal 23: 1415-1423.
40. Vanni C, Ottaviano C, Guo F, Puppo M, Varesio L, Zheng Y, Eva A,. 2005. Constitutively active Cdc42 mutant confers growth disadvantage in cell transformation. Cell Cycle 4: 1675-1682.
41. Wang L, Yang L, Debidda M, Witte D, Zheng Y,. 2007. Cdc42 GTPase-activating protein deficiency promotes genomic instability and premature aging-like phenotypes. Proc Natl Acad Sci USA 104: 1248-1253.
42. Wang SW, Norbury C, Harris AL, Toda T,. 1999. Caffeine can override the S-M checkpoint in fission yeast. J Cell Sci 112 (Pt 6): 927-937.
43. Williams CL,. 2003. The polybasic region of Ras and Rho family small GTPases: a regulator of protein interactions and membrane association and a site of nuclear localization signal sequences. Cell Signal 15: 1071-1080.
44. Yang W,. 2011. Surviving the sun: Repair and bypass of DNA UV lesions. Protein Sci 20: 1781-1789.
45. Yasuda S, Taniguchi H, Oceguera-Yanez F, Ando Y, Watanabe S, Monypenny J, Narumiya S,. 2006. An essential role of Cdc42-like GTPases in mitosis of HeLa cells. FEBS letters 580: 3375-3380.
46. Yeh YM, Pan YT, Wang TC,. 2005. Cdc42/Rac1 participates in the control of telomerase activity in human nasopharyngeal cancer cells. Cancer Lett 218: 207-213.