Aggregation of monocytes and platelets interferes in measurement of monocyte viability with phosphatidylserine expression but not with Mitochondrial membrane potential in whole blood

Cytometry Part B - Clinical Cytometry

Volumn 92, Issue 3, 2017, Pages 228-235

  Amundsen, Erik K ^a   Urdal, Petter ^a   Holthe, Mette R ^a   Henriksson, Carola E ^a  

^a ULLEVÅL UNIVERSITY HOSPITAL   (Norway)

Author keywords

aggregates; apoptosis; monocytes; platelet; whole blood

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE; EDETIC ACID; LACTADHERIN; PHOSPHATIDYLSERINE; PROCOAGULANT; LIPOPOLYSACCHARIDE; MEMBRANE ANTIGEN; MFGE8 PROTEIN, HUMAN; MILK PROTEIN; THROMBOPLASTIN;

ANTICOAGULATION; ARTICLE; CELL CULTURE; CELL DEATH; CELL VIABILITY; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MEMBRANE PERMEABILITY; MITOCHONDRIAL MEMBRANE POTENTIAL; MONOCYTE; OUTER MEMBRANE; PRIORITY JOURNAL; PROTEIN EXPRESSION; THROMBOCYTE AGGREGATION; BLOOD; BLOOD CLOTTING; CELL SURVIVAL; DRUG EFFECTS; GENETICS; METABOLISM; PROCEDURES; THROMBOCYTE;

ANTIGENS, SURFACE; BLOOD COAGULATION; BLOOD PLATELETS; CELL DEATH; CELL SURVIVAL; FLOW CYTOMETRY; HUMANS; LIPOPOLYSACCHARIDES; MEMBRANE POTENTIAL, MITOCHONDRIAL; MILK PROTEINS; MONOCYTES; PHOSPHATIDYLSERINES; PLATELET AGGREGATION; THROMBOPLASTIN;

EID: 84937231056     PISSN: 15524949     EISSN: 15524957     Source Type: Journal    
DOI: 10.1002/cyto.b.21189     Document Type: Article

References (21)

1. Henriksson CE, Klingenberg O, Ovstebo R, Joo GB, Westvik AB, Kierulf P. Discrepancy between tissue factor activity and tissue factor expression in endotoxin-induced monocytes is associated with apoptosis and necrosis. Thromb Haemost 2005;94:1236–1244.
2. Bach RR. Tissue factor encryption. Arterioscler Thromb Vasc Biol 2006;26:456–461.
3. Ovstebo R, Hellum M, Aass HC, Troseid AM, Brandtzaeg P, Mollnes TE, Henriksson CE. Microparticle-associated tissue factor activity is reduced by inhibition of the complement protein 5 in Neisseria meningitidis-exposed whole blood. Innate Immun 2014;20:552–560.
4. Gyulkhandanyan AV, Mutlu A, Freedman J, Leytin V. Markers of platelet apoptosis: Methodology and applications. J Thromb Thrombolysis 2012;33:397–411.
5. McFarland DC, Zhang C, Thomas HC, R TL. Confounding effects of platelets on flow cytometric analysis and cell-sorting experiments using blood-derived cells. Cytometry A 2006;69A:86–94.
6. Ouk C, Jayat-Vignoles C, Donnard M, Feuillard J. Both CD62 and CD162 antibodies prevent formation of CD36-dependent platelets, rosettes, and artefactual pseudoexpression of platelet markers on white blood cells: A study with ImageStream(R). Cytometry A 2011;79A:477–484.
7. Basavaraj MG, Osterud B, Hansen JB. Influence of different anticoagulants on monocyte procoagulant functions and monocyte-platelet aggregates formation. J Thromb Haemost 2012;10:1698–1702.
8. Chen M, Geng JG. P-selectin mediates adhesion of leukocytes, platelets, and cancer cells in inflammation, thrombosis, and cancer growth and metastasis. Arch Immunol Ther Exp (Warsz) 2006;54:75–84.
9. Durham AC. A survey of readily available chelators for buffering calcium ion concentrations in physiological solutions. Cell Calcium 1983;4:33–46.
10. Key NS, Mackman N. Tissue factor and its measurement in whole blood, plasma, and microparticles. Semin Thromb Hemost 2010;36:865–875.
11. Hodge GL, Flower R, Han P. Optimal storage conditions for preserving granulocyte viability as monitored by Annexin V binding in whole blood. J Immunol Methods 1999;225:27–38.
12. Tait JF, Smith C, Wood BL. Measurement of phosphatidylserine exposure in leukocytes and platelets by whole-blood flow cytometry with annexin V. Blood Cells Mol Dis 1999;25:271–278.
13. Telford WG, King LE, Fraker PJ. Rapid quantitation of apoptosis in pure and heterogeneous cell populations using flow cytometry. J Immunol Methods 1994;172:1–16.
14. Kroemer G, Galluzzi L, Vandenabeele P, Abrams J, Alnemri ES, Baehrecke EH, Blagosklonny MV, El-Deiry WS, Golstein P, Green DR, et al. Classification of cell death: Recommendations of the Nomenclature Committee on Cell Death 2009. Cell Death Differ 2009;16:3–11.
15. Shi J, Shi Y, Waehrens LN, Rasmussen JT, Heegaard CW, Gilbert GE. Lactadherin detects early phosphatidylserine exposure on immortalized leukemia cells undergoing programmed cell death. Cytometry A 2006;69A:1193–1201.
16. Lund PK, Joo GB, Westvik AB, Ovstebo R, Kierulf P. Isolation of monocytes from whole blood by density gradient centrifugation and counter-current elutriation followed by cryopreservation: Six years' experience. Scand J Clin Lab Invest 2000;60:357–365.
17. Fossati G, Moulding DA, Spiller DG, Moots RJ, White MR, Edwards SW. The mitochondrial network of human neutrophils: Role in chemotaxis, phagocytosis, respiratory burst activation, and commitment to apoptosis. J Immunol 2003;170:1964–1972.
18. McCarthy DA, Macey MG, Allen PD. A simple, novel, procedure for monitoring membrane scrambling and permeability in microparticles, platelets, and leukocytes in whole blood samples. Exp Hematol 2008;36:909–921.
19. Jakel A, Plested JS, Hoe JC, Makepeace K, Gidney MA, Lacelle S, St MF, Cox AD, Richards JC, Moxon ER. Naturally-occurring human serum antibodies to inner core lipopolysaccharide epitopes of Neisseria meningitidis protect against invasive meningococcal disease caused by isolates displaying homologous inner core structures. Vaccine 2008;26:6655–6663.
20. Remer KA, Brcic M, Jungi TW. Toll-like receptor-4 is involved in eliciting an LPS-induced oxidative burst in neutrophils. Immunol Lett 2003;85:75–80.
21. Tsukamoto H, Fukudome K, Takao S, Tsuneyoshi N, Kimoto M. Lipopolysaccharide-binding protein-mediated Toll-like receptor 4 dimerization enables rapid signal transduction against lipopolysaccharide stimulation on membrane-associated CD14-expressing cells. Int Immunol 2010;22:271–280.