Rapid transfer of overexpressed integral membrane protein from the host membrane into soluble lipid nanodiscs without previous purification

Biological Chemistry

Volumn 396, Issue 8, 2015, Pages 903-915

  Shirzad Wasei, Nazhat ^a   Van Oostrum, Jenny ^a   Bovee Geurts, Petra H M ^a   Kusters, Lisanne J A ^a   Bosman, Giel J C G M ^a   DeGrip, Willem J ^a  

^a RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

baculovirus expression system; his tagged rhodopsin; membrane protein solubilization; membrane scaffold protein; protein stabilization; recombinant protein

Indexed keywords

DETERGENT; GUANINE NUCLEOTIDE BINDING PROTEIN; MEMBRANE SCAFFOLD PROTEIN; N DODECYL BETA DEXTRO MALTOSIDE; NANODISC; OPSIN; RECOMBINANT PROTEIN; RHODOPSIN; SCAFFOLD PROTEIN; SOLUBLE LIPID NANODISC; TRANSDUCIN; UNCLASSIFIED DRUG; LIPID BILAYER; MEMBRANE PROTEIN; NANOMATERIAL;

AFFINITY CHROMATOGRAPHY; ARTICLE; DYNAMIC LIGHT SCATTERING; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IMMUNOBLOTTING; LIGHT SCATTERING; LIPID BILAYER; NONHUMAN; PHOTOLYSIS; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN TRANSPORT; REGENERATION; SOLUBILIZATION; CHEMISTRY; GENE EXPRESSION; ISOLATION AND PURIFICATION;

CHROMATOGRAPHY, AFFINITY; GENE EXPRESSION; LIPID BILAYERS; MEMBRANE PROTEINS; NANOSTRUCTURES; RHODOPSIN;

EID: 84936818615     PISSN: 14316730     EISSN: 14374315     Source Type: Journal    
DOI: 10.1515/hsz-2015-0100     Document Type: Article

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