Characterization of aflatoxigenic Aspergillus flavus and A. parasiticus strain isolates from animal feedstuffs in northeastern Iran

Iranian Journal of Veterinary Research

Volumn 16, Issue 2, 2015, Pages 150-155

  Davari, E ^a   Mohsenzadeh, M ^a   Mohammadi, G ^a   Rezaeian Doloei, R ^b  

^a FERDOWSI UNIVERSITY OF MASHHAD   (Iran)

^b ISLAMIC AZAD UNIVERSITY   (Iran)

Author keywords

Aflatoxin; Aspergillus spp.; Feedstuff; Multiplex PCR

Indexed keywords

ANIMALIA; ASPERGILLUS; ASPERGILLUS FLAVUS; ASPERGILLUS NIGER; ASPERGILLUS ORYZAE; ASPERGILLUS PARASITICUS;

EID: 84936757130     PISSN: 17281997     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Accensi, E; Abarca, ML and Cabanes, FJ (2004). Occurrence of Aspergillus species in mixed feeds and component raw materials and their ability to produce ochratoxin A. Food Microb., 21: 623-627.
2. Chang, PK; Horn, BW and Dorner, JW (2005). Sequence breakpoints in the aflatoxin biosynthesis gene cluster and flanking regions in nonaflatoxigenic Aspergillus flavus isolates. Fungal Genet. Biol., 42: 914-923.
3. Chang, PK; Yu, J; Bhatnagar, D and Cleveland, TE (1999a). The carboxy-terminal portion of the aflatoxin pathway regulatory protein AFLR of Aspergillus parasiticus activates GAL1:lacZ gene expression in Saccharomyces cerevisiae. Appl. Environ. Microbiol., 65: 2508-2512.
4. Chang, PK; Yu, J; Bhatnagar, D and Cleveland, TE (1999b). Repressor-AFLR interaction modulates aflatoxin biosynthesis in Aspergillus parasiticus. Mycopathologia. 147: 105-112.
5. Criseo, G; Bagnara, A and Bisignano, G (2001). Differentiation of aflatoxin-producing and non-producing strain of Aspergillus flavus group. J. App. Microb., 33: 291-295.
6. Ehrlich, KC; Montalbano, BG; Bhatnagar, D and Cleveland, TE (1998). Alteration of different domains in AFLR affects aflatoxin pathway metabolism in Aspergillus parasiticus transformants. Fungal Genet. Biol., 23: 279-287.
7. Ehrlich, KC and Yu, J (2010). Aflatoxin-like gene clusters and how they evolved. In: Rai, M and Varma, A (Eds.), Mycotoxins in food, feed, and bioweapons. New York, Springer. PP: 65-75.
8. Erami, M; Hashemi, SJ; Pourbakhsh, SA; Shahsavandi, S; Mohammadi, S; Shooshtari, AH and Jahanshiri, Z (2007). Application of PCR on detection of aflatoxinogenic fungi. Arch. Razi Inst., 62: 95-100.
9. Ghiasian, SA and Maghsood, AH (2011). Occurrence of aflatoxigenic fungi in cow feeds during the summer and winter season in Hamadan, Iran. Afr. J. Microbiol. Res., 5: 516-521.
10. Keller, NP; Kantz, NJ and Adams, TH (1994). Aspergillus nidulans verA is required for production of the mycotoxin sterigmatocystin. Appl. Environ. Microbiol., 60: 1444-1450.
11. Khosravi, AR; Shokri, H; Yahyaraeyat, R and Soltani, M (2004). Isolation of toxigenic and nontoxigenic fungi from feedstuffreferred to the center of mycology. J. Vet. Res., 59: 221-226.
12. Klich, MA (2002). Identification of common Aspergillus species. Utrecht, Netherlands, CBS. P: 116.
13. Konietzny, U and Greiner, R (2003). The application of PCR in the detection of mycotoxigenic fungi in foods. Braz. J. Microbiol., 34: 283-300.
14. Macioro, KG; Herrera, P; Jones, FT; Pillai, SD and Ricke, SC (2007). Effect on poultry and livestock of feed contamination with bacteria and fungi. Anim. Feed Sci. Tech., 133: 109-136.
15. Mehrzad, J; Klein, G; Kamphues, J; Wolf, P; Grabowski, N and Schuberth, HJ (2011). In vitro effects of very low levels of aflatoxin B1 on free radicals production and bactericidal activity of bovine blood neutrophils. Vet. Immunol. Immunopatol., 141: 16-25.
16. Moubasher, H; Abutaleb, A and Senousy, HH (2013). Molecular differentiation between aflatoxinogenic and non-aflatoxinogenic strains of Aspergillus flavus and Aspergillus parasiticus. Microbiology. 82: 642-646.
17. Pitt, JI and Hocking, AD (2009). Fungi and food spoilage. 3rd Edn., New York, Springer-Verlag. PP: 3-9.
18. Rahimi, P; Sharifnabi, B and Bahar, M (2008). Detection of aflatoxin in Aspergillus species isolated from pistachio in Iran. Phytopathology. 156: 15-20.
19. Rashid, M; Khalil, S; Ayub, N; Ahmed, W and Ghaffarkhan, A (2008). Categorization of Aspergillus flavus and Aspergillus parasiticus isolates of stored wheat grains in to aflatoxinogenics and non-aflatoxinogenics. Pak. J. Bot., 40: 2177-2192.
20. Rodrigues, P; Armando, V; Kozakiewicz, Z and Lima, N (2009). A polyphasic approach to the identification of aflatoxigenic and non-aflatoxigenic strain of Aspergillus section flavi isolation from Portuguesa almond. Food Microbiol., 129: 187-193.
21. Rosa, CAR; Cavaglieri, LR; Ribeiro, JMM; Keller, KM; Alonso, VA; Chiacchiera, SM and Dalcero, AM (2008). Mycobiota and naturally-occurring ochratoxin A in dairy cattle feed from Rio de Janeiro state, Brazil. World Mycotoxin J., 1: 195-201.
22. Saleemi, MK; Khan, MZ; Ahrar, K and Javed, I (2010). Mycoflora of poultry feeds and mycotoxins producing potential of Aspergillus species. Pak. J. Bot., 42: 427-434.
23. Shapira, R; Paster, N; Eyal, O; Menashero, M; Mett, A and Salomon, R (1996). Detection of aflatoxigenic mold in grain by PCR. App. Environ. Microbiol., 62: 3270-3273.
24. Somashekar, D; Rati, ER; Anad, S and Chandrashekar, A (2004). Isolation, enumeration and PCR characterization of aflatoxigenic fungi from food and feed samples in India. Food Microb., 21: 809-813.
25. van der Vossen, JM and Hofstra, H (1996). DNA based typing, identification and detection systems for food spoilage microorganisms: development and implementation. Int. J. Food Microbiol., 33: 35-49.
26. Woloshuk, CP; Foutz, KR; Brewer, JF; Bhatnagar, D; Cleveland, TE and Payne, GA (1994). Molecular characterization of aflR, a regulatory locus for aflatoxin biosynthesis. App. Environ. Microbiol., 60: 2408-2414.
27. Yelton, MM; Hamer, JE and Timberlake, WE (1984). Transformation of Aspergillus nidulans by using a trpC plasmid. Proceedings of the National Academy of Sciences of the USA. 81: 1470-1474.
28. Yu, J (2012). Current understanding on aflatoxin biosynthesis and future perspective in reducing aflatoxin contamination. Toxins. 4: 1024-1057.
29. Yu, J; Bhatnagar, D and Cleveland, TE (2004a). Completed sequence of aflatoxin pathway gene cluster in Aspergillus parasiticus. FEBS Lett., 564: 126-130.
30. Yu, J; Chang, PK; Ehrlich, KC; Cary, JW; Bhatnagar, D; Cleveland, TE; Payne, GA; Linz, JE; Woloshuk, CP and Bennett, JW (2004b). Clustered pathway genes in aflatoxin biosynthesis. Appl. Environ. Microbiol., 70: 1253-1262.