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Volumn 13, Issue 24, 2015, Pages 6681-6685

Biofunction-assisted aptasensors based on ligand-dependent 3′ processing of a suppressor tRNA in a wheat germ extract

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EID: 84935897512     PISSN: 14770520     EISSN: None     Source Type: Journal    
DOI: 10.1039/c5ob00794a     Document Type: Article
Times cited : (7)

References (30)
  • 17
    • 79951519285 scopus 로고    scopus 로고
    • A. Ogawa RNA 2011 17 478
    • (2011) RNA , vol.17 , pp. 478
    • Ogawa, A.1
  • 23
    • 84935864710 scopus 로고    scopus 로고
    • This high suppression efficiency comparable to that of t86 was likely attributable to the moderately long 3′ trailer being favorable for RNase Z-mediated cleavage. It has been reported that a much longer 3′ trailer adversely affects the RNase Z-mediated cleavage; see
    • A. Ogawa Y. Doi Org. Biomol. Chem. 2015 13 1008
    • (2015) Org. Biomol. Chem. , vol.13 , pp. 1008
    • Ogawa, A.1    Doi, Y.2
  • 26
    • 0030752211 scopus 로고    scopus 로고
    • The suppression efficiency in the OFF state (2.6% of that by 3pt) was as low as those of the th-free probes Given the fact that the maturation efficiency of a 5′ premature sup-tRNA (5pt) was 21% with 1 h incubation in WGE, the structural change was expected to be faster than the 5′ processing (k = 2.7 × 10-2 min-1), which was slightly slower than the degradation of 3pt (k = 5-7 × 10-2 min-1). 9
    • G. R. Zimmermann R. D. Jenison C. L. Wick J.-P. Simorre A. Pardi Nat. Struct. Biol. 1997 4 644
    • (1997) Nat. Struct. Biol. , vol.4 , pp. 644
    • Zimmermann, G.R.1    Jenison, R.D.2    Wick, C.L.3    Simorre, J.-P.4    Pardi, A.5
  • 28
    • 0027995790 scopus 로고
    • Another explanation is that a tRNA probe with a much longer 3′ trailer is less susceptible to the RNase-Z mediated cleavage. 10 However, tc(th1)-MS(4) was well processed in the ON state, even at low concentrations of the ligand Nonetheless, the suppression efficiency in the OFF state was still lower than that of 3pt-IS(14), due to the longer 3′ dangling end However, a much longer aptamer may slightly inhibit the RNase Z-mediated cleavage in the ON state. 10
    • P. Burgstaller M. Famulok Angew. Chem., Int. Ed. Engl. 1994 33 1084
    • (1994) Angew. Chem., Int. Ed. Engl. , vol.33 , pp. 1084
    • Burgstaller, P.1    Famulok, M.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.