Micropatterning cell adhesion on polyacrylamide hydrogels

Methods in Molecular Biology

Volumn 1066, Issue , 2013, Pages 147-156

  Zhang, Jian ^a   Guo, Wei Hui ^a   Rape, Andrew ^a   Wang, Yu Li ^a  

^a CARNEGIE MELLON UNIVERSITY   (United States)

Author keywords

Cell adhesion; Cell geometry; Mechanotransduction; Substrate rigidity

Indexed keywords

COLLAGEN; FIBRONECTIN; GELATIN; POLYACRYLAMIDE;

ARTICLE; CELL ADHESION; CELL STRAIN 3T3; CHEMICAL BINDING; ELECTRON MICROSCOPY; HYDROGEL; MECHANOTRANSDUCTION; PRIORITY JOURNAL; RIGIDITY; SURFACE PROPERTY; TISSUE ENGINEERING;

3T3 CELLS; ACRYLIC RESINS; ANIMALS; CELL ADHESION; CELL COMMUNICATION; CELL LINE; CELL MOVEMENT; HYDROGELS; MECHANOTRANSDUCTION, CELLULAR; MICE; STRESS, MECHANICAL;

EID: 84934444361     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-604-7_13     Document Type: Article

References (21)

1. Tambe DT, Corey-Hardin C, Angelini TE et al (2011) Collective cell guidance by cooperative intercellular forces. Nat Mater 10:469-475
2. Janmey PA, Miller RT (2011) Mechanisms of mechanical signaling in development and disease. J Cell Sci 124:9-18
3. Chen CS, Mrksich M, Huang S et al (1997) Geometric control of cell life and death. Science 276:1425-1428
4. Singhvi R, Kumar A, Lopez G et al (1994) Engineering cell shape and function. Science 264:696-698
5. Dike L, Chen C, Mrksich M et al (1999) Geometric control of switching between growth, apoptosis, and differentiation during angiogenesis using micropatterned substrates. In Vitro Cell Dev Biol Anim 35: 441-448
6. McBeath R, Pirone DM, Nelson CM et al (2004) Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment. Dev Cell 6:483-495
7. Kilian KA, Bugarija B, Lahn BT et al (2010) Geometric cues for directing the differentiation of mesenchymal stem cells. Proc Natl Acad Sci 107:4872-4877
8. Wang H-B, Dembo M, Wang Y-L (2000) Substrate fl exibility regulates growth and apoptosis of normal but not transformed cells. Am J Physiol Cell Physiol 279:C1345-C1350
9. Engler AJ, Sen S, Sweeney HL et al (2006) Matrix elasticity directs stem cell lineage specifi cation. Cell 126:677-689
10. Pelham RJ, Wang Y-l (1997) Cell locomotion and focal adhesions are regulated by substrate fl exibility. Proc Natl Acad Sci 94:13661-13665
11. Rape A, Guo W-H, Wang Y-L (2011) Responses of cells to adhesion-mediated signals: A universal mechanism. In: Wagoner JA, Harley BAC (eds) Mechanobiology of cell-cell and cell-matrix interactions. Springer, USA, pp 1-10
12. Wang Y-L, Pelham RJ Jr (1998) Preparation of a fl exible, porous polyacrylamide substrate for mechanical studies of cultured cells. In: Richard BV (ed) Methods in enzymology. Academic, New York, pp 489-496
13. Damljanovic V, Lagerholm BC, Jacobson K (2005) Bulk and micropatterned conjugation of extracellular matrix proteins to characterized polyacrylamide substrates for cell mechanotransduction assays. Biotechniques 39:847-851
14. Guo W-h, Wang Y-I (2007) Retrograde fl uxes of focal adhesion proteins in response to cell migration and mechanical signals. Mol Biol Cell 18:4519-4527
15. Wang N, Ostuni E, Whitesides GM et al (2002) Micropatterning tractional forces in living cells. Cell Motil Cytoskeleton 52:97-106
16. Vignaud T, Galland R, Tseng Q et al (2012) Reprogramming cell shape with laser nanopatterning. J Cell Sci 125:2134-2140
17. Rape AD, Guo W-h, Wang Y-l (2011) The regulation of traction force in relation to cell shape and focal adhesions. Biomaterials 32:2043-2051
18. Fischer B, Thomas KB, Dorner F (1998) Collagen covalently immobilized onto plastic surfaces simplifi es measurement of von Willebrand factor-collagen binding activity. Ann Hematol 76:159-166
19. Li F, Li B, Wang Q-M et al (2008) Cell shape regulates collagen type I expression in human tendon fi broblasts. Cell Motil Cytoskeleton 65:332-341
20. Reinhart-King CA, Dembo M, Hammer DA (2003) Endothelial cell traction forces on RGD-derivatized polyacrylamide substrata. Langmuir 19:1573-1579
21. Guo W-H, Wang Y-l (2011) Micropatterning cell-substrate adhesions using linear polyacrylamide as the blocking agent. Cold Spring Harb Protoc 2011(3):prot5582