Expression and purification of recombinant antibody formats and antibody fusion proteins

Methods in Molecular Biology

Volumn 1131, Issue , 2014, Pages 273-295

  Siegemund, Martin ^a   Richter, Fabian ^a   Seifert, Oliver ^a   Unverdorben, Felix ^a   Kontermann, Roland E ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Antibody; E. coli; Fc fusion; FLAG; Fusion protein; HEK293; IMAC; scFv; Strep

Indexed keywords

ANTIBODY; ANTIBODY FUSION PROTEIN; HYBRID PROTEIN; NITRILOTRIACETATE NICKEL; POLYHISTIDINE TAG; PROTEIN A; RECOMBINANT ANTIBODY; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL STRAIN; BINDING KINETICS; CARBOXY TERMINAL SEQUENCE; CELL AGGREGATION; CELL LYSATE; CENTRIFUGATION; COLUMN CHROMATOGRAPHY; EMBRYO; ESCHERICHIA COLI; EXPRESSION VECTOR; HUMAN; HUMAN CELL; MAMMAL CELL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

ANTIBODIES; CELL LINE; ESCHERICHIA COLI; HUMANS; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS;

EID: 84934443662     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-992-5_18     Document Type: Article

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