In vitro reconstitution of the MHC class i peptide-loading complex

Methods in Molecular Biology

Volumn 960, Issue , 2013, Pages 67-79

  Wearsch, Pamela A ^a   Cresswell, Peter ^b  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Antigen processing; MHC class I; Peptide loading; Tapasin

Indexed keywords

CALRETICULIN; GLYCAN; HETERODIMER; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; TAPASIN; CARRIER PROTEIN; HLA ANTIGEN CLASS 1; PDIA3 PROTEIN, HUMAN; PEPTIDE FRAGMENT; PROTEIN DISULFIDE ISOMERASE;

ANTIGEN PRESENTATION; ARTICLE; COMPLEX FORMATION; IN VITRO STUDY; PRIORITY JOURNAL; PROTEIN PROCESSING; ANIMAL; BACULOVIRUS; CHEMISTRY; GENETICS; HUMAN; IMMUNOLOGY; IMMUNOPRECIPITATION; METABOLISM; PROTEIN MULTIMERIZATION; PROTEIN STABILITY; SF9 CELL; SOLUBILITY; SPODOPTERA;

ANIMALS; BACULOVIRIDAE; CALRETICULIN; HISTOCOMPATIBILITY ANTIGENS CLASS I; HUMANS; IMMUNOPRECIPITATION; MEMBRANE TRANSPORT PROTEINS; PEPTIDE FRAGMENTS; PROTEIN DISULFIDE-ISOMERASES; PROTEIN MULTIMERIZATION; PROTEIN STABILITY; SF9 CELLS; SOLUBILITY; SPODOPTERA;

EID: 84934443423     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-218-6_6     Document Type: Article

References (22)

1. Wearsch P, Peaper D, Cresswell P (2011) Essential glycan-dependent interactions optimize MHC class I peptide loading. Proc Natl Acad Sci U S A 108:4950-4955
2. Del Cid N, Jeffery E, Rizvi S, Stamper E, Peters L, Brown W, Provoda C, Raghavan M (2010) Modes of calreticulin recruitment to the major histocompatibility complex class I assembly pathway. J Biol Chem 285:4520-4535
3. Dong G, Wearsch PA, Peaper DR, Cresswell P, Reinisch KR (2009) Insights into MHC class I peptide loading from the structure of the tapasin- ERp57 thiol oxidoreductase heterodimer. Immunity 16:21-32
4. Garbi N, Tan P, Diehl AD, Chambers BJ, Ljunggren HG, Momburg F, Hammerling GJ (2000) Impaired immune responses and altered peptide repertoire in tapasin-deficient mice. Nat Immunol 1:234-238
5. Grandea AG III, Golovina TN, Hamilton SE, Sriram V, Spies T, Brutkiewicz RR, Harty J, Eisenlohr LC, Van Kaer L (2000) Impaired assembly yet normal trafficking of MHC class I molecules in tapasin mutant mice. Immunity 13:213-222
6. Williams AP, Peh CA, Purcell AW, McCluskey J, Elliott T (2002) Optimization of the MHC class I peptide cargo is dependent on tapasin. Immunity 16(4):509-520
7. Chen M, Bouvier M (2007) Analysis of interactions in a tapasin/class I complex provides a mechanism for peptide selection. EMBO J 26:1681-1690
8. Wearsch PA, Cresswell P (2007) Selective loading of high-af fi nity peptides onto major histocompatibility complex class I molecules by the tapasin-ERp57 heterodimer. Nat Immunol 8: 873-881
9. Ortmann B, Androlewicz MJ, Cresswell P (1994) MHC class I/beta 2-microglobulin complexes associate with TAP transporters before peptide binding. Nature 368(6474): 864-867
10. Suh WK, Cohen-Doyle MF, Fruh K, Wang K, Peterson PA, Williams DB (1994) Interaction of MHC class I molecules with the transporter antigen processing. Science 264(5163): 1322-1326
11. Bangia N, Lehner PJ, Hughes EA, Surman M, Cresswell P (1999) The N-terminal region of tapasin is required to stabilize the MHC class I loading complex. Eur J Immunol 29:1858-1870
12. Garbi N, Tiwari N, Momburg F, Hammerling GJ (2003) A major role for tapasin as a stabilizer of the TAP peptide transporter and consequences for MHC class I expression. Eur J Immunol 33:264-273
13. Lehner PJ, Surman MJ, Cresswell P (1998) Soluble tapasin restores MHC class I expression and function in the tapasin-negative cell line.220. Immunity 8:221-231
14. Leonhardt RM, Keusekotten K, Bekpen C, Knittler MR (2005) Critical role for the tapasin- docking site of TAP2 in the functional integrity of the MHC class I-peptide-loading complex. J Immunol 175:5104-5114
15. Vigneron N, Peaper D, Leonhardt R, Cresswell P (2009) Functional significance of tapasin membrane association and disulfide linkage to ERp57 in MHC class I presentation. Eur J Immunol 39:2371-2376
16. Sadasivan B, Lehner PJ, Ortmann B, Spies T, Cresswell P (1996) Roles for calreticulin and a novel glycoprotein, tapasin, in the interaction of MHC class I molecules with TAP. Immunity 5:103-114
17. Wearsch PA, Jakob CA, Vallin A, Dwek RA, Rudd PM, Cresswell P (2004) Major histocompatibility complex class I molecules expressed with monoglucosylated N-linked glycans bind calreticulin independently of their assembly status. J Biol Chem 279:25112-25121
18. Dick TP, Bangia N, Peaper DR, Cresswell P (2002) Disul fi de bond isomerization and the assembly of MHC class I-peptide complexes. Immunity 16:87-98
19. Peaper DR, Wearsch PA, Cresswell P (2005) Tapasin and ERp57 form a stable disul fi delinked dimer within the MHC class I peptideloading complex. EMBO J 24:3613-3623
20. Suh W, Mitchell E, Yang Y, Peterson P, Waneck G, Williams D (1996) MHC class I molecules form ternary complexes with calnexin and undergo peptide-regulated interaction with TAP via their domains. J Exp Med 184(2): 337-348
21. Harris MR, Yu YY, Kindle CS, Hansen TH, Solheim JC (1998) Calreticulin and calnexin interact with different protein and glycan determinants during the assembly of MHC class I. J Immunol 160:5404-5409
22. Rodenko B, Toebes M, Hadrup S, van Esch W, Molenaar A, Schumacher T, Ovaa H (2006) Generation of peptide-MHC class I complexes through UV-mediated ligand exchange. Nat Protoc 1:1120-1132