Mirna and shrna expression vectors based on mrna and mirna processing

Methods in Molecular Biology

Volumn 936, Issue , 2013, Pages 195-207

  Wu, Ping ^a   Wilmarth, Melissa A ^a   Zhang, Feng ^a   Du, Guangwei ^a  

^a UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

Author keywords

Intron; Microrna; Psm155; Psm30; Rna Interference; Small Hairpin Rna

Indexed keywords

COMPLEMENTARY DNA; ENHANCED GREEN FLUORESCENT PROTEIN; MESSENGER RNA; MICRORNA; PYROXYLIN; SHORT HAIRPIN RNA; SMALL INTERFERING RNA;

ARTICLE; CELL TYPE; CLONING; DNA SEQUENCE; EXPRESSION VECTOR; FEMALE; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; INTRON; MUTATION RATE; OPEN READING FRAME; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA INTERFERENCE; RNA PROCESSING; RNA SPLICING;

EID: 84934442809     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-83-0_16     Document Type: Article

References (8)

1. Brummelkamp TR, Bernards R, Agami R (2002) A system for stable expression of short interfering RNAs in mammalian cells. Science 296:550-553
2. Paddison PJ, Caudy AA, Bernstein E, Hannon GJ, Conklin DS (2002) Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev 16:948-958
3. Chung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL (2006) Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. Nucleic Acids Res 34:e53
4. Silva JM, Li MZ, Chang K, Ge W, Golding MC, Rickles RJ, Siolas D, Hu G, Paddison PJ, Schlabach MR, Sheth N, Bradshaw J, Burchard J, Kulkarni A, Cavet G, Sachidanandam R, McCombie WR, Cleary MA, Elledge SJ, Hannon GJ (2005) Second-generation shRNA libraries covering the mouse and human genomes. Nat Genet 37:1281-1288
5. Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ (2005) A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Proc Natl Acad Sci USA 102:13212-13217
6. de Fougerolles A, Vornlocher HP, Maraganore J, Lieberman J (2007) Interfering with disease: a progress report on siRNA-based therapeutics. Nat Rev Drug Discov 6:443-453
7. Du G, Yonekubo J, Zeng Y, Osisami M, Frohman MA (2006) Design of expression vectors for RNA interference based on miRNAs and RNA splicing. FEBS J 273: 5421-5427
8. Wu J, Bonsra AN, Du G (2008) pSM155 and pSM30 vectors for miRNA and shRNA expression. In: Sioud M (ed) siRNA and miRNA