Detecting microRNA in human cancer tissues with fluorescence in situ hybridization

Methods in Molecular Biology

Volumn 1039, Issue , 2013, Pages 19-27

  Shi, Zonggao ^a   Johnson, Jeff J ^a   Stack, M Sharon ^a  

^a UNIVERSITY OF NOTRE DAME   (United States)

Author keywords

Fluorescence in situ hybridization (FISH); Formalin fixed paraffin embedded (FFPE) tissue; microRNA; Squamous cell carcinoma

Indexed keywords

MICRORNA;

ARTICLE; CANCER TISSUE; CONTROLLED STUDY; FLUORESCENCE IN SITU HYBRIDIZATION; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MOUTH CANCER; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; TISSUE PREPARATION;

HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; MICRORNAS; NEOPLASMS; PARAFFIN EMBEDDING; TISSUE FIXATION;

EID: 84934441681     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-535-4_2     Document Type: Article

References (13)

1. Lee RC, Feinbaum RL, Ambros V (1993) The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 75:843-854
2. Andorfer CA, Necela BM, Thompson EA, Perez EA (2011) MicroRNA signatures: clinical biomarkers for the diagnosis and treatment of breast cancer. Trends Mol Med 17: 313-319
3. Qavi AJ, Kindt JT, Bailey RC (2010) Sizing up the future of microRNA analysis. Anal Bioanal Chem 398:2535-2549
4. Nielsen BS (2012) MicroRNA in situ hybridization. Methods Mol Biol 822:67-84
5. Shi Z, Johnson JJ, Stack MS (2012) Fluorescence in situ hybridization for microRNA detection in archived oral cancer tissues. J Oncol 2012:903581
6. McKay JA, Murray GI, Keith WN, McLeod HL (1997) Amplification of fluorescent in situ hybridisation signals in formalin fixed paraffin wax embedded sections of colon tumour using biotinylated tyramide. Mol Pathol 50:322-325
7. Bobrow MN, Moen PT Jr (2001) Tyramide signal amplification (TSA) systems for the enhancement of ISH signals in cytogenetics. Curr Protoc Cytom Chapter 8, Unit 8 9
8. Helander KG (1994) Kinetic studies of formaldehyde binding in tissue. Biotech Histochem 69:177-179
9. Fox CH, Johnson FB, Whiting J, Roller PP (1985) Formaldehyde fixation. J Histochem Cytochem 33:845-853
10. Glud M, Klausen M, Gniadecki R, Rossing M, Hastrup N, Nielsen FC et al (2009) MicroRNA expression in melanocytic nevi: the usefulness of formalin-fixed, paraffin-embedded material for miRNA microarray profiling. J Invest Dermatol 129:1219-1224
11. Liu A, Tetzlaff MT, Vanbelle P, Elder D, Feldman M, Tobias JW et al (2009) MicroRNA expression profiling outperforms mRNA expression profiling in formalin-fixed paraffinembedded tissues. Int J Clin Exp Pathol 2:519-527
12. Nuovo GJ (2010) In situ detection of microRNAs in paraffin embedded, formalin fixed tissues and the co-localization of their putative targets. Methods 52:307-315
13. Kloosterman WP, Wienholds E, de Bruijn E, Kauppinen S, Plasterk RH (2006) In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes. Nat Methods 3:27-29