Establishment and characterization of monoclonal antibodies against SARS coronavirus

Methods in Molecular Biology

Volumn 454, Issue , 2008, Pages 191-203

  Ohnishi, Kazuo ^a  

^a NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

Author keywords

Antigen capture sandwich ELISA; Confocal immunofluorescence; Coronavirus; Immunization; Monoclonal antibody; SARS CoV; Western blot

Indexed keywords

CORONAVIRUS; MUS; SARS CORONAVIRUS;

EID: 84934435223     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-181-9_15     Document Type: Article

References (12)

1. Drosten, C., Gunther, S., Preiser, W., van der Werf, S., Brodt, H. R., Becker, S., Rabenau, H., Panning, M., Kolesnikova, L., Fouchier, R. A., et al. (2003) Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N. Engl. J. Med. 348, 1967-1976.
2. Ksiazek, T. G., Erdman, D., Goldsmith, C. S., Zaki, S. R., Peret, T., Emery, S., Tong, S., Urbani, C., Comer, J. A., Lim, W., et al. (2003) A novel coronavirus associated with severe acute respiratory syndrome. N. Engl. J. Med. 348, 1953-1966.
3. Peiris, J. S., Chu, C. M., Cheng, V. C., Chan, K. S., Hung, I. F., Poon, L. L., Law, K. I., Tang, B. S., Hon, T. Y., Chan, C. S., et al. (2003) Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia: a prospective study. Lancet 361, 1767-1772.
4. Chan, P. K., To, W .K., Ng, K. C., Lam, R. K., Ng, T. K., Chan, R. C., Wu, A., Yu,W. C., Lee, N., Hui, D. S., et al. (2004) Laboratory diagnosis of SARS. Emerg. Infect. Dis. 10, 825-831.
5. Wang, W. K., Chen, S. Y., Liu, I. J., Chen, Y. C., Chen, H. L., Yang, C. F., Chen, P. J., Yeh, S. H., Kao, C. L., Huang, L. M., et al. (2004) Detection of SARS-associated coronavirus in throat wash and saliva in early diagnosis. Emerg. Infect. Di.s 10, 1213-1219.
6. Ohnishi, K., Sakaguchi, M., Kaji, T., Akagawa, K., Taniyama, T., Kasai, M., Tsunetsugu-Yokota, Y., Oshima, M., Yamamoto, K., Takasuka, N., et al. (2005) Immunological detection of severe acute respiratory syndrome coronavirus by monoclonal antibodies. Jpn. J. Infect. Dis. 58, 88-94.
7. Che, X. Y., Qiu, L. W., Pan, Y. X., Wen, K., Hao, W., Zhang, L. Y., Wang, Y. D., Liao, Z. Y., Hua, X., Cheng, V. C., et al. (2004) Sensitive and specific monoclonal antibody-based capture enzyme immunoassay for detection of nucleocapsid antigen in sera from patients with severe acute respiratory syndrome. J. Clin. Microbiol. 42, 2629-2635.
8. Tsunetsugu-Yokota, Y., Ato, M., Takahashi, Y., Hashimoto, S.-I., Kaji, T., Kuraoka, M., Yamamoto, K.-I., Mitsuki, Y.-Y., Yamamoto, T., Ohshima, M., et al. (2007) Formalin-treated UV-inactivated SARS coronavirus vaccine retains its immunogenicity and promotes Th2-type immune responses. Jpn. J. Infect. Dis. In press.
9. Kozbor, D., and Roder, J. C. (1984) In vitro stimulated lymphocytes as a source of human hybridomas. Eur. J. Immunol. 14, 23-27.
10. Harlow, E., and Lane, D. (1998) Using Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, New York.
11. Shepherd, P., and Dean, C. (2000) Monoclonal Antibodies: A Practical Approach: Oxford University Press, New York.
12. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.