Analyzing oligomerization of individual transmembrane helices and of entire membrane proteins in e. coli : A hitchhiker's guide to GALLEX

Methods in Molecular Biology

Volumn 932, Issue , 2013, Pages 259-276

  Cymer, Florian ^a   Sanders, Charles R ^a   Schneider, Dirk ^a  

^a NONE

Author keywords

GALLEX; Helix helix interaction; In vivo; Membrane protein; Oligomerization; TOXCAT

Indexed keywords

EID: 84934434836     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-65-6_16     Document Type: Article

References (30)

1. von Heijne G (2011) Introduction to theme "membrane protein folding and insertion". Annu Rev Biochem 80:157-160
2. von Heijne G (2011) Membrane proteins: from bench to bits. Biochem Soc Trans 39:747-750
3. Popot JL, Engelman DM (1990) Membrane protein folding and oligomerization: the twostage model. Biochemistry 29:4031-4037
4. Engelman DM, Chen Y, Chin CN et al (2003) Membrane protein folding: beyond the two stage model. FEBS Lett 555:122-125
5. Prodöhl A, Volkmer T, Finger C et al (2005) De fi ning the structural basis for assembly of a transmembrane cytochrome. J Mol Biol 350:744-756
6. Cymer F, Anbazhagan V, Schneider D (2011) Transmembrane helix-helix interactions are modulated by the sequence context and by lipid bilayer properties. Biochim Biophys Acta 1818:963-73
7. Cymer F, Schneider D (2009) A single glutamate residue controls the oligomerization, function, and stability of the aquaglyceroporin GlpF. Biochemistry 49:279-286
8. Berkefeld H, Fakler B, Schulte U (2010) Ca 2+ - activated K + channels: from protein complexes to function. Physiol Rev 90:1437-1459
9. Bornhovd C, Vogel F, Neupert W et al (2006) Mitochondrial membrane potential is dependent on the oligomeric state of F1F0-ATP synthase supracomplexes. J Biol Chem 281:13990-13998
10. Bowie JU (2005) Solving the membrane protein folding problem. Nature 438:581-589
11. Schneider D, Finger C, Prodöhl A et al (2007) From interactions of single transmembrane helices to folding of a-helical membrane proteins: analyzing transmembrane helix-helix interactions in bacteria. Curr Protein Pept Sci 8:45-61
12. Russ WP, Engelman DM (1999) TOXCAT: a measure of transmembrane helix association in a biological membrane. Proc Natl Acad Sci USA 96:863-868
13. Langosch D, Brosig B, Kolmar H et al (1996) Dimerisation of the glycophorin A transmembrane segment in membranes probed with the ToxR transcription activator. J Mol Biol 263:525-530
14. Schneider D, Engelman DM (2003) GALLEX, a measurement of heterologous association of transmembrane helices in a biological membrane. J Biol Chem 278:3105-3111
15. Finger C, Escher C, Schneider D (2009) The single transmembrane domains of human receptor tyrosine kinases encode self-interactions. Sci Signal 2:ra56
16. Schneider D, Engelman DM (2004) Involvement of transmembrane domain interactions in signal transduction by a/b integrins. J Biol Chem 279:9840-9846
17. Schneider D, Engelman DM (2004) Motifs of two small residues can assist but are not suf fi cient to mediate transmembrane helix interactions. J Mol Biol 343:799-804
18. Escher C, Cymer F, Schneider D (2009) Two GxxxG-like motifs facilitate promiscuous interactions of the human ErbB transmembrane domains. J Mol Biol 389:10-16
19. Finger C, Volkmer T, Prodöhl A et al (2006) The stability of transmembrane helix interactions measured in a biological membrane. J Mol Biol 358:1221-1228
20. Prodöhl A, Weber M, Dreher C et al (2007) A mutational study of transmembrane helix-helix interactions. Biochimie 89:1433-1437
21. Dmitrova M, Younes-Cauet G, Oertel- Buchheit P et al (1998) A new LexA-based genetic system for monitoring and analyzing protein heterodimerization in Escherichia coli. Mol Gen Genet 257:205-212
22. Treptow NA, Shuman HA (1985) Genetic evidence for substrate and periplasmic-bindingprotein recognition by the MalF and MalG proteins, cytoplasmic membrane components of the Escherichia coli maltose transport system. J Bacteriol 163:654-660
23. Sambrook J, Russel DW (2001) Cold Spring Habour Press USA. Cold Sping Harbor, New York, NY
24. Miller JH (1992) Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York, NY
25. Lau FW, Bowie JU (1997) A method for assessing the stability of a membrane protein. Biochemistry 36:5884-5892
26. Kolmar H, Hennecke F, Gotze K et al (1995) Membrane insertion of the bacterial signal transduction protein ToxR and requirements of transcription activation studied by modular replacement of different protein substructures. EMBO J 14:3895-3904
27. Lis M, Blumenthal K (2006) A modi fi ed, dual reporter TOXCAT system for monitoring homodimerization of transmembrane segments of proteins. Biochem Biophys Res Commun 339:321-324
28. King G, Dixon AM (2010) Evidence for role of transmembrane helix-helix interactions in the assembly of the Class II major histocompatibility complex. Mol Biosyst 6:1650-1661
29. Volkmer T, Becker C, Prodöhl A et al (2006) Assembly of a transmembrane b-type cytochrome is mainly driven by transmembrane helix interactions. Biochim Biophys Acta 1758:1815-1822
30. Fuhrmann E, Bultema JB, Kahmann U et al (2009) The vesicle-inducing protein 1 from Synechocystis sp. PCC 6803 organizes into diverse higher-ordered ring structures. Mol Biol Cell 20:4620-4628