Brightness experiments

Methods in Molecular Biology

Volumn 1076, Issue , 2014, Pages 699-718

  MacDonald, Patrick J ^a   Johnson, Jolene ^a   Chen, Yan ^a   Mueller, Joachim D ^a  

^a University of Minnesota   (United States)

Author keywords

Afterpulsing; Autocorrelation; Dead time; Fluctuation; Photobleaching; Photon count moments; Stoichiometry; Titration; Two photon

Indexed keywords

FLUORESCENT DYE;

ARTICLE; BLEACHING; BRIGHTNESS; CALIBRATION; CELL COMPONENT; CELL ORGANELLE; CELL SELECTION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOMETRY; FLUORESCENCE; LIGHT; MEASUREMENT ERROR; PRIORITY JOURNAL; PROTEIN LOCALIZATION; STOICHIOMETRY; WATER IMMERSION;

ANIMALS; CERCOPITHECUS AETHIOPS; COS CELLS; FLUORESCENCE; GREEN FLUORESCENT PROTEINS; HUMANS; PHOTOBLEACHING; PHOTONS; PROTEIN BINDING; PROTEINS; SPECTROMETRY, FLUORESCENCE;

EID: 84934434484     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-649-8_32     Document Type: Article

References (11)

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2. Chen Y, Wei L-N, Mueller JD (2003) Probing protein oligomerization in living cells with fluorescence fluctuation spectroscopy. Proc Natl Acad Sci USA 100:15492-15497
3. Wu B, Chen Y, Mueller JD (2009) Fluorescence fluctuation spectroscopy of mCherry in living cells. Biophys J 96:2391-2404
4. Day RN, Davidson MW (2009) The fluorescent protein palette: tools for cellular imaging. Chem Soc Rev 38:2887
5. Day RN, Davidson MW (2009) Aequorea victoria fluorescent proteins. Zeiss Online Campus. http://zeiss-campus.magnet.fsu.edu/articles/probes/jellyfishfps. html. Accessed 01 September 2012
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7. Macdonald PJ, Chen Y, Wang X et al (2010) Brightness analysis by z-scan fluorescence fluctuation spectroscopy for the study of protein interactions within living cells. Biophys J 99:979-988
8. Chen Y, Mueller JD, Ruan Q et al (2002) Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy. Biophys J 82:133-144
9. Wu B,Mueller JD(2005) Time-integrated fluorescence cumulant analysis in fluorescence fluctuation spectroscopy. Biophys J 89:2721-2735
10. Hillesheim LN, Mueller JD (2003) The photon counting histogram in fluorescence fluctuation spectroscopy with non-ideal photodetectors. Biophys J 85:1948-1958
11. Sanchez-Andres A, Chen Y, Mueller JD (2005) Molecular brightness determined from a generalized form of Mandel's Q-parameter. Biophys J 89:3531-3547