United in death - Related by blood? Genetic and archeometric analyses of skeletal remains from the neolithic earthwork bruchsal-aue

American Journal of Physical Anthropology

Volumn 157, Issue 3, 2015, Pages 458-471

  Keller, Marcel ^a   Rott, Andreas ^b   Hoke, Nadja ^a   Schwarzberg, Heiner ^c   Regner Kamlah, Birgit ^d   Harbeck, Michaela ^b   Wahl, Joachim ^e  

^a UNIVERSITY OF MUNICH   (Germany)

^b BAVARIAN NATURAL HISTORY COLLECTIONS   (Germany)

^c Bavarian State Archaeological Collection   (Germany)

^d State Regional Authority Stuttgart   (Germany)

^e State Regional Authority Stuttgart   (Germany)

Author keywords

kinship; Michelsberg Culture; mtDNA; stable isotopes; STRs

Indexed keywords

AMELOGENIN; CARBON; CARBON 14; DNA; MITOCHONDRIAL DNA; NITROGEN; STRONTIUM; ISOTOPE; MICROSATELLITE DNA;

ADULT; ARTICLE; CAUSE OF DEATH; CHILD; DEATH; DNA DETERMINATION; DNA EXTRACTION; FEMALE; GENETIC ANALYSIS; GERMANY; HAPLOGROUP; HAPLOTYPE; HEALTH STATUS; HUMAN; LIFE HISTORY; MALE; MASS SPECTROMETRY; MORPHOLOGY; NEOLITHIC; OSTEOLOGY; RADIOMETRIC DATING; SHORT TANDEM REPEAT; STATISTICAL ANALYSIS; Y CHROMOSOME; ARCHEOLOGY; BONE; CHEMISTRY; GENETICS; INFANT; MIDDLE AGED; PHYSICAL ANTHROPOLOGY; PRESCHOOL CHILD;

ADULT; ANTHROPOLOGY, PHYSICAL; ARCHAEOLOGY; BONE AND BONES; CHILD; CHILD, PRESCHOOL; CHROMOSOMES, HUMAN, Y; DNA, MITOCHONDRIAL; FEMALE; GERMANY; HUMANS; INFANT; ISOTOPES; MALE; MICROSATELLITE REPEATS; MIDDLE AGED;

EID: 84931955666     PISSN: 00029483     EISSN: 10968644     Source Type: Journal    
DOI: 10.1002/ajpa.22738     Document Type: Article

References (83)

1. Adler C-P., 1998. Knochenkrankheiten. Diagnostik makroskopischer, histologischer und radiologischer Strukturveränderungen des Skeletts. Berlin Heidelberg New York: Springer.
2. Alterauge S., 2013. Silobestattungen aus unbefestigten Siedlungen der Michelsberger Kultur in Süd- und Südwestdeutschland-Versuch einer Annährerung. In:, Müller-Scheeßel N, editor. Irregulärè Bestattungen in der Urgeschichte: Norm, Ritual, Strafe...? Kolloquien zur Vor- und Frühgeschichte 19. Bonn: Habelt. p 185-196.
3. Ambrose SH,. 1990. Preparation and characterization of bone and tooth collagen for isotopic analysis. J Archaeol Sci 17: 431-451.
4. Andersen NH., 1997. The Sarup enclosures. Jutland Archaeological Society Publications 33. Aarhus: Aarhus University Press.
5. TM PCR amplification kit: the new miniSTR multiplex kit. Forensic Sci Int Genet Suppl Ser 1: 89-91.
6. Andrews RM, Kubacka I, Chinnery PF, Lightowlers RN, Turnbull DM, Howell N,. 1999. Reanalysis and revision of the Cambridge reference sequence for human mitochondrial DNA. Nat Genet 23: 147.
7. Behrends RH., 1991. Erdwerke der Jungsteinzeit in Bruchsal. Neuere Forschungen 1983-1991. Archäologische Informationen aus Baden-Württemberg 22. Stuttgart: Gesellschaft für Vor- und Frühgeschichte in Württemberg und Hohenzollern.
8. Bentley RA,. 2006. Strontium isotopes from the earth to the archaeological skeleton: a review. J Archaeol Method Theor 13: 135-187.
9. Bollongino R, Nehlich O, Richards MP, Orschiedt J, Thomas MG, Sell C, Fajko Z, Powell A, Burger J,. 2013. 2000 years of parallel societies in stone age central Europe. Science 342: 479-481.
10. Brandt G, Haak W, Adler CJ, Roth C, Szécsényi-Nagy A, Karimnia S, Möller-Rieker S, Meller H, Ganslmeier R, Friederich S, Dresely V, Nicklisch N, Pickrell JK, Sirocko F, Reich D, Cooper A, Alt KW,. 2013. Ancient DNA reveals key stages in the formation of central European mitochondrial genetic diversity. Science 342: 257-261.
11. Bronk Ramsey C, Scott EM, van der Plicht J,. 2013. Calibration for archaeological and environmental terrestrial samples in the time range 26-50 k cal BP. Radiocarbon 55: 2021-2027.
12. Buikstra JE, Ubelaker DH., 1997. Standards for data collection from human skeletal remains. Fayetteville: Arkansas Archaeological Survey.
13. Burt NM,. 2013. Stable isotope ratio analysis of breastfeeding and weaning practices of children from medieval Fishergate House York, UK. Am J Phys Anthropol 152: 407-416.
14. Cooper A, Poinar HN,. 2000. Ancient DNA: do it right or not at all. Science 289: 1139b.
15. DeNiro MJ,. 1985. Postmortem preservation and alteration of in vivo bone collagen isotope ratios in relation to palaeodietary reconstruction. Nature 317: 806-809.
16. DeWitte SN,. 2010. Sex differentials in frailty in medieval England. Am J Phys Anthropol 143: 285-297.
17. DeWitte SN, Wood JW,. 2008. Selectivity of the black death with respect to preexisting health. Proc Natl Acad Sci USA 105: 1436-1441.
18. Drábek J,. 2009. Validation of software for calculating the likelihood ratio for parentage and kinship. Forensic Sci Int Genet 3: 112-118.
19. Dürrwächter C, Craig OE, Collins MJ, Burger J, Alt KW,. 2006. Beyond the grave: variability in neolithic diets in Southern Germany? J Archaeol Sci 33: 39-48.
20. Egeland T, Mostad PF, Mevâg B, Stenersen M,. 2000. Beyond traditional paternity and identification cases. Selecting the most probable pedigree. Forensic Sci Int 110: 47-59.
21. Feranec RS, Hadly EA, Paytan A,. 2007. Determining landscape use of holocene mammals using strontium isotopes. Oecologia 153: 943-950.
22. Ferembach D, Schwidetzky I, Stloukal M,. 1979. Empfehlungen für die Alters- und Geschlechtsdiagnose am Skelett. Homo 30: 1-32.
23. Fuller BT, Fuller JL, Harris D, Hedges RM,. 2006. Detection of breastfeeding and weaning in modern human infants with carbon and nitrogen stable isotope ratios. Am J Phys Anthropol 129: 279-293.
24. Gamba C, Fernández E, Tirado M, Pastor F, Arroyo-Pardo E,. 2011. Brief communication: ancient nuclear DNA and kinship analysis: the case of a medieval burial in San Esteban Church in Cuellar (Segovia, Central Spain). Am J Phys Anthropol 144: 485-491.
25. Gilbert MTP, Djurhuus D, Melchior L, Lynnerup N, Worobey M, Wilson AS, Andreasen C, Dissing J,. 2007. mtDNA from hair and nail clarifies the genetic relationship of the 15th century Qilakitsoq Inuit mummies. Am J Phys Anthropol 133: 847-853.
26. Grupe G, Heinrich D, Peters J,. 2009. A brackish water aquatic foodweb: trophic levels and salinity gradients in the Schlei fjord, Northern Germany, in Viking and medieval times. J Archaeol Sci 36: 2125-2144.
27. Haak W, Brandt G, de Jong HN, Meyer C, Ganslmeier R, Heyd V, Hawkesworth C, Pike AWG, Meller H, Alt KW,. 2008. Ancient DNA, strontium isotopes, and osteological analyses shed light on social and kinship organization of the Later Stone Age. Proc Natl Acad Sci USA 105: 18226-18231.
28. Harbeck M, Seifert L, Hänsch S, Wagner DM, Birdsell D, Parise KL, Wiechmann G, Grupe G, Thomas A, Keim P, Zöller L, Bramanti B, Riehm JM, Scholz HC,. 2013. Yersinia pestis form skeletal remains from the 6th century AD reveals insights into Justinianic plague. PLoS Pathogens 9: e1003349.
29. Harbeck M, von Heyking K., 2014. Die Ernährung menschlicher Bevölkerungen in Spätantike und Mittelalter auf dem Gebiet der ehemaligen Raetia secunda und des Herzogtums Baiern.-Welche Hinweise können Isotopenanalysen geben ? In:, Drauschke J, Prien R, Reis A, editors. Küche und Keller in Antike und Frühmittelalter. Studien zu Spätantike und Frühmittelalter. Hamburg: Dr. Kovač;. p 197-222.
30. Heaton THE, Jones G, Halstead P, Tsipropoulos T,. 2009. Variations in the 13C/12C ratios of modern wheat grain, and implications for interpreting data from Bronze Age Assiros Toumba, Greece. J Archaeol Sci 36: 2224-2233.
31. Hobson KA, Alisaukas RT, Clark RG,. 1993. Stable-nitrogen isotope enrichment in avian tissues due to fasting and nutritional stress: Implications for isotopic analyses of diet. Condor 95: 388-394.
32. Hofreiter M, Jaenicke V, Serre D, von Haeseler A, Pääbo S,. 2001. DNA sequences from multiple amplifications reveal artifacts induced by cytosine deamination in ancient DNA. Nucleic Acids Res 29: 4793-4799.
33. Höhn B., 2002. Michelsberger Kultur in der Wetterau. Universitätsforschungen zur Prähistorischen Archäologie 87. Bonn: Habelt.
34. Jeunesse C., 2010. Die Michelsberger Kultur. Eine Kultur ohne Friedhöfe. In:, Lichter C, editor. Jungsteinzeit im Umbruch. Die "Michelsberger Kultur" und Mitteleuropa vor 6000 Jahren. Katalog zur Ausstellung im Badischen Landesmuseum Schloss Karlsruhe 20.11.2010-15.5.2011. Darmstadt: Primus. p 90-95.
35. Jeunesse C, Seidel U., 2010. Die Erdwerke. In:, Lichter C, editor. Jungsteinzeit im Umbruch. Die "Michelsberger Kultur" und Mitteleuropa vor 6000 Jahren. Katalog zur Ausstellung im Badischen Landesmuseum Schloss Karlsruhe 20.11.2010-15.5.2011. Darmstadt: Primus. p 58-69.
36. Kayser M, Sajantila A,. 2001. Mutations at Y-STR loci: Implications for paternity testing and forensic analysis. Forensic Sci Int 118: 116-121.
37. Lacan M, Keyser C, Ricaut F, Brucato N, Duranthon F, Guilaine J,. 2011a. Ancient DNA reveals male diffusion through the Neolithic Mediterranean route. Proc Natl Acad Sci USA 108: 9788-9791.
38. Lacan M, Keyser C, Ricaut F-X, Brucato N, Tarrús J, Bosch A, Guilaine J, Crubézy E, Ludes B,. 2011b. Ancient DNA suggests the leading role played by men in the Neolithic dissemination. Proc Natl Acad Sci USA 108: 18255-18259.
39. Lee EJ, Krause-Kyora B, Rinne C, Schütt R, Harder M, Müller J, von Wurmb-Schwark N, Nebel A,. 2014. Ancient DNA insights from the Middle Neolithic in Germany. Archaeol Anthropol Sci 6: 199-204.
40. Lee HY, Song I, Ha E, Cho S-B, Yang WI, Shin K-J,. 2008. mtDNAmanager: A web-based tool for the management and quality analysis of mitochondrial DNA control-region sequences. BMC Bioinform 9: 483.
41. Lüning J., 1968. Die Michelsberger Kultur. Ihre Funde in zeitlicher und räumlicher Gliederung. Berichte der Römisch-Germanischen Kommission 48. Berlin: De Gruyter.
42. Martin R., 1928. Lehrbuch der Anthropologie in systematischer Darstellung. Jena: Gustav Fischer.
43. Meindl R, Lovejoy CO,. 1985. Ectocranial suture closure. A revised method for the determination of skeletal age at death based on the lateral anterior sutures. Am J Phys Anthropol 68: 57-66.
44. Melchior L, Gilbert MTP, Kivisild T, Lynnerup N, Dissing J,. 2008. Rare mtDNA haplogroups and genetic differences in rich and poor Danish Iron-age villages. Am J Phys Anthropol 125: 206-215.
45. Meyer C, Ganslmeier R, Dresely V, Alt KW,. 2010. New approaches to the reconstruction of kinship and social structure based on bioarchaeological analysis of Neolithic multiple and collective graves. In:, Kolář J, Trampota F, editors. Theoretical and methodological considerations in Central European Neolithic Archaeology. BAR International Series 2325. Oxford: Archaeopress. p 11-23.
46. Meyer C, Karimnia S, Knipper C, Stecher M, Brandt G, Schlenker B, Ramsthaler F, Alt KW., 2013. Eine komplexe Mehrfachbestattung der Salzmünder Kultur. In:, Meller H, editor. 3300 BC. Mysteriöse Steinzeittote und ihre Welt. Halle (Saale): Nünnerich-Asmus. p 290-299.
47. Meyer C, Kranzbühler J, Drings S, Bramanti B, Nehlich O, Richards MP, Alt KW., 2008. Die menschlichen Skelettfunde aus der neolithischen Totenhütte von Benzingerode. Anthropologische Untersuchungen an den Bestattungen eines Kollektivgrabes der Bernburger Kultur. In:, Meller H, editor. Die Totenhütte von Benzingerode: Archäologie und Anthropologie. Archäologie in Sachsen-Anhalt-Sonderband 7. Halle (Saale): Landesmuseum für Vorgeschichte. p 107-161.
48. Nemeskéri J, Harsányi L, Acsádi G,. 1960. Methoden zur Diagnose des Lebensalters von Skelettfunden. Anthropol Anzeiger 24: 70-95.
49. Neuberger FM, Jopp E, Graw M, Püschel K, Grupe G,. 2013. Signs of malnutrition and starvation-reconstruction of nutritional life histories by serial isotopic analyses of hair. Forensic Sci Int 226: 22-32.
50. Newsome SD, Phillips DL, Culleton BJ, Guilderson TP, Koch PL,. 2004. Dietary reconstruction of an early to middle holocene human population from the central California coast: insights from advanced stable isotope mixing models. J Archaeol Sci 31: 1101-1115.
51. Ortner DJ, Putschar WGJ,. 1981. Identification of pathological conditions in human skeletal remains. Smithsonian Contributions to Anthropology. No. 28. Washington D.C.: Smithsonian Institution Press.
52. Orschiedt J., 1999. Manipulationen an menschlichen Skelettresten. Taphonomische Prozesse, Sekundärbestattungen oder Anthropophagie? Urgeschichtliche Materialhefte 13. Tübingen: Mo Vince.
53. Pääbo S, Poinar HN, Serre D, Jaenicke-Despres V, Hebler J, Rohland N, Kuch M, Krause J, Vigilant L, Hofreiter M,. 2004. Genetic analyses from ancient DNA. Annu Rev Genet 38: 645-679.
54. Phillips DL, Koch PL,. 2002. Incorporating concentration dependence in stable isotope mixing models. Oecologia 130: 114-125.
55. Pin C, Joannon S, Bosq C, Le Fèvre B, Gauthier PJ,. 2003. Precise determination of Rb, Sr, Ba and Pb in geological materials by isotope dilution and ICP-quadrupole mass spectrometry following selective separation of the analytes. J Anal Spectrom 18: 135-141.
56. Price TD, Wahl J, Knipper C, Burger-Heinrich E, Kurz G, Bentley RA,. 2003. Das bandkeramische Gräberfeld vom "Viesenhäuser Hof" bei Stuttgart-Mühlhausen: Neue Untersuchungsergebnisse zum Migrationsverhalten im Frühen Neolithikum. Fundberichte aus Baden-Württemberg 27: 1-36.
57. Pruvost M, Grange T, Geigl E-M,. 2005. Minimizing DNA contamination by using UNG-coupled quantitative real-time PCR on degraded DNA samples: Application to ancient DNA studies. Biotechniques 38: 569-575.
58. Regner-Kamlah B., 2009. Die Befunde des Michelsberger Grabenwerkes Bruchsal-Aue, Lkr. Karlsruhe. In:, Schmotz K, editor. Vorträge des 27. Niederbayerischen Archäologentages. Rahden/Westf.: Marie Leidorf. p 65-90.
59. Regner-Kamlah B,. 2010. Grabenumbauten im Michelsberger Erdwerk von Bruchsal "Aue" (lkr. Karlsruhe). Vorbericht zur Auswertung. Archäol Korrespondenzblatt 40: 175-190.
60. Reimer PJ, Bard E, Bayliss A, Beck JW, Blackwell PG, Bronk C, Caitlin R, Hai EB, Edwards RL, Friedrich M, Grootes PM, Guilderson TP, Haflidason H, Hajdas I, Hatté C, Heaton TJ, Hoffmann DL, Hogg AG, Hughen KA, Kromer B, Manning SW, Niu M, Reimer RW, Richards DA, Scott EM, Southon JR, Staff RA, Turney CSM, van der Plicht J,. 2013. Intcal13 and Marine13 radiocarbon age calibration curves 0-50,000 years cal BP. Radiocarbon 55: 1869-1887.
61. Sampietro ML, Lao O, Caramelli D, Lari M, Pou R, Martí M, Bertranpetit J, Lalueza-Fox C,. 2007. Palaeogenetic evidence supports a dual model of Neolithic spreading into Europe. Proc R Soc Biol Sci 274: 2161-2167.
62. Scheeres M, Knipper C, Hauschild M, Schönfelder M, Siebel W, Vitali D, Pare C, Alt KW,. 2013. Evidence for "Celtic migrations"? Strontium isotope analysis at the early La Tène" (LT B) cemeteries of Nebringen (Germany and Monte Bibele (Italy). J Archaeol Sci 40: 3614-3625.
63. Schultz M., 1988. Paläopathologische Diagnostik. In:, Knussmann R, editor. Anthropologie. Handbuch der vergleichenden Biologie des Menschen Bd. 1. Stuttgart, New York: Gustav Fischer. p 480-496.
64. Schutkowski H,. 1989. Beitrag zur Alters- und Geschlechtsdiagnose am Skelett nichterwachsener Individuen. Anthropol Anzeiger 47: 1-9.
65. Seidel U., 2008. Michelsberger Erdwerke im Raum Heilbronn. Materialhefte zur Archäologie in Baden-Württemberg 81/3. Stuttgart: Theiss.
66. Seifert L, Harbeck M, Thomas A, Hoke N, Zöller L, Wiechmann I, Grupe G, Scholz HC, Riehm JM,. 2013. Strategy for sensitive and specific detection of Yersinia pestis in skeletons of the Black Death pandemic. PLoS One 8: e75742.
67. Simón M, Jordana X, Armentano N, Santos C, Díaz N, Solórzano E, López JB, González-Ruiz M, Malgosa A,. 2011. The presence of nuclear families in prehistoric collective burials revisited: The Bronze Age burial of Montanissell Cave (Spain) in the light of aDNA. Am J Phys Anthropol 146: 406-413.
68. Šlaus M,. 2000. Biocultural analysis of sex differences in mortality profiles and stress levels in the late medieval population from Nova Rača, Croatia. Am J Phys Anthropol 111: 193-209.
69. Sofeso C, Vohberger M, Wisnowsky A, Päffgen B, Harbeck M., 2012. Verifying archaeological hypotheses: Investigantions on origin and genealogical lineages of a privileged society in Upper Bavaria from Imperial Roman times (Erding, Kletthamer Feld). In: Burger J, Kaiser E, Schier W, editors. Population dynamics in pre- and Early History. New approaches by using stable isotopes and genetics. proceedings of the conference in Berlin, March 24-26, 2010. Berlin/Boston: De Gruyter. p 113-130.
70. Thurzo M, Obertova Z,. 2008. Relationship between cribra orbitalia and enamel hypoplasia in the Early Medieval Slavic population at Borovce, Slovakia. Int J Osteoarchaeol 18: 280-292.
71. Wahl J., 1994. Zur Ansprache und Definition von Sonderbestattungen. In: Beiträge zur Archäozoologie und Prähistorischen Anthropologie. 8. Arbeitstreffen der Osteologen, Konstanz 1993 im Andenken an Joachim Boessneck. Forschungen und Berichte zur Vor- und Frühgeschichte in Baden-Württemberg 53. Stuttgart: Theiss. p 85-106.
72. Wahl J., 2007. Karies, Kampf und Schädelkult. 150 Jahre anthropologische Forschung in Südwestdeutschland. Materialhefte zur Archäologie in Baden-Württemberg 79. Stuttgart: Theiss.
73. Wahl J., 2008. Profan oder kultisch-bestattet oder entsorgt? Die menschlichen Skelettreste aus den Michelsberger Erdwerken von Heilbronn-Klingenberg, Neckarsulm-Obereisesheim und Ilsfeld. In:, Schlenker B, Stephan E, Wahl J, editors. Michelsberger Erdwerke im Raum Heilbronn. Materialhefte zur Archäologie in Baden-Württemberg 81/3. Stuttgart: Theiss. p 703-848.
74. Wahl J., 2010. Wenige Knochen, viele Fragen. Auf der Suche nach den Menschen der Michelsberger Kultur. In:, Lichter C, editor. Jungsteinzeit im Umbruch. Die "Michelsberger Kultur" und Mitteleuropa vor 6000 Jahren. Katalog zur Ausstellung im Badischen Landesmuseum Schloss Karsruhe 20.11.2010-15.5.2011. Darmstadt: Primus. p 96-101.
75. Wahl J., 2012. In: Wahl J. 15000 Jahre Mord und Totschlag. Stuttgart: Theiss.
76. Wahl J, Höhn B,. 1988. Eine Mehrfachbestattung der Michelsberger Kultur aus Heidelberg-Handschuhsheim, Rhein-Neckar-Kreis. Fundberichte Aus Baden-Württemberg 13: 123-198.
77. Walker PL, Bathurst RR, Richman R, Gjerdrum T, Andrushko VA,. 2009. The causes of porotic hyperostosis and cribra orbitalia: A reappraisal of the iron-deficiency-anemia hypothesis. Am J Phys Anthropol 139: 109-125.
78. Warnberg O, Alt KW., 2012. Molekulargenetische Analysen an den Bestattungen aus dem endneolithischen Kollektivgrab von Spreitenbach. In:, Doppler T, editor. Spreitenbach-Moosweg (Aargau, Schweiz): Ein Kollektivgrab um 2500 v. Chr. Antiqua 51. Basel: Archäologie Schweiz. p 158-169.
79. White TD, Folkens PA., 1991. Human osteology. San Diego: Academic Press.
80. Wiechmann I, Grupe G,. 2005. Detection of Yersinia pestis DNA in two medieval skeletal finds from Aschheim (Upper Bavaria, 6th century AD). Am J Phys Anthropol 126: 48-55.
81. Wiechmann I, Harbeck M, Grupe G, Peters J., 2013. Ancient DNA facility at the ArchaeoBioCenter, LMU Munich. In:, Grupe G, McGlynn G, Peters J, editors. Current discoveries from outside and within. Documenta archaeobiologiae 10. Rahden/Westf.: Marie Leidorf. p 233-236.
82. Winters M, Barta JL, Monroe C, Kemp BM,. 2011. To clone or not to clone: Method analysis for retrieving consensus sequences in ancient DNA samples. PLoS One 6: e21247.
83. Yang DY, Eng B, Waye JS, Dudar JC, Saunders SR,. 1998. Technical note: Improved DNA extraction from ancient bones using silica-based spin columns. Am J Phys Anthropol 105: 539-543.