Technical note: Improved DNA extraction from ancient bones using silica- based spin columns

American Journal of Physical Anthropology

Volumn 105, Issue 4, 1998, Pages 539-543

  Yang, Dongya Y ^a   Eng, Barry ^a   Waye, John S ^a   Dudar, J Christopher ^a   Saunders, Shelley R ^a  

^a MCMASTER UNIVERSITY   (Canada)

Author keywords

Ancient bone; DNA extraction; PCR inhibitors; Silica based spin columns

Indexed keywords

ORGANIC SOLVENT; PROTEINASE; SILICON DIOXIDE;

AGING; ARTICLE; BONE; DNA DETERMINATION; DNA PURIFICATION; GENE AMPLIFICATION; POLYMERASE CHAIN REACTION;

ANIMALS; BONE AND BONES; DNA; FOSSILS; HUMANS; POLYMERASE CHAIN REACTION; SILICON DIOXIDE; SPECIMEN HANDLING;

EID: 0031921276     PISSN: 00029483     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1096-8644(199804)105:4<539::AID-AJPA10>3.0.CO;2-1     Document Type: Article

References (8)

1. Hagelberg E and Clegg JB (1991) Isolation and characterization of DNA from archaeological bone. Proc. R. Soc. Lond. [Biol.] 244:45-52.
2. Herrmann B and Hummel S (eds.) (1993) Ancient DNA. New York: Springer-Verlag.
3. Höss M and Pääbo S (1993) DNA extraction from Pleistocene bones by a silica-based purification method. Nucleic Acids Res. 21:3913-3914.
4. Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, and Arnheim N (1985) Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350-1354.
5. Warburton PE, Greig GM, Haaf T, and Willard HF (1991) PCR amplification of chromosome-specific alpha satellite DNA: definition of centromeric STS markers and polymorphic analysis. Genomics 11:324-333.
6. Waye JS and Willard HF (1986) Structure, organization, and sequence of alpha satellite DNA from human chromosome 17: evidence for evolution by unequal crossing-over and an ancestral pentamer repeat shared with the human X chromosome. Mol. Cell. Biol. 6:3156-2165.
7. Yang DY, Eng B, Dudar JC, Saunders SR, and Waye JS (1997) Removal of PCR inhibitors using silica-based spin columns: application to ancient bones. Can. Soc. For. Sci. J. 30:1-5.
8. Yang DY (1997) DNA diagnosis of thalassemia from ancient Italian skeletons. Ph.D. Thesis, McMaster University, Hamilton, Ontario, Canada (submitted).