Strep-Tagged Protein Purification

Methods in Enzymology

Volumn 559, Issue , 2015, Pages 53-69

  Maertens, Barbara ^a   Spriestersbach, Anne ^a   Kubicek, Jan ^a   Schäfer, Frank ^a  

^a QIAGEN GMBH   (Germany)

Author keywords

Baculovirus infected insect cells; E. coli lysates; Microscale purification; Strep Tactin magnetic beads; Strep tag system; Strep tagged protein purification; Transfected mammalian cells

Indexed keywords

AGAROSE; BACTERIAL PROTEIN; PROTEIN TAG; RESIN; ALA-TRP-ARG-HIS-PRO-GLN-PHE-GLY-GLY; LIGAND; OLIGOPEPTIDE; PROTEIN; RECOMBINANT PROTEIN; SEPHAROSE; STREPTAVIDIN;

ARTICLE; AUTOMATION; BINDING AFFINITY; CELL LYSATE; ESCHERICHIA COLI; FAST PROTEIN LIQUID CHROMATOGRAPHY; GENETIC TRANSFECTION; GRAVITY; INSECT CELL; LYSIS; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; STREP TAG SYSTEM; AFFINITY CHROMATOGRAPHY; ANIMAL; BACULOVIRIDAE; CELL LINE; CHEMISTRY; DEVICES; INSECT; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; MAGNETISM; METABOLISM; PROCEDURES;

HEXAPODA; MAMMALIA;

ANIMALS; BACULOVIRIDAE; CELL LINE; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, LIQUID; ESCHERICHIA COLI; INSECTS; LIGANDS; MAGNETICS; OLIGOPEPTIDES; PROTEINS; RECOMBINANT PROTEINS; SEPHAROSE; STREPTAVIDIN;

EID: 84931574966     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/bs.mie.2014.11.008     Document Type: Chapter

References (19)

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4. QIAGEN Strep-Tag Protein Handbook: For Expressing, Purifying and Detecting Proteins Carrying a Strep-Tag II 3rd edn. 2009 QIAGEN GmbH Hilden, Germany
5. A. Skerra, and T.G. Schmidt Use of the Strep-tag and streptavidin for detection and purification of recombinant proteins Methods in Enzymology 326 2000 271 304
6. QIAGEN Strep-Tag Protein Handbook: For Expressing, Purifying and Detecting Proteins Carrying a Strep-Tag II 3rd edn. 2009 QIAGEN GmbH Hilden, Germany
7. A. Skerra, and T.G. Schmidt Use of the Strep-tag and streptavidin for detection and purification of recombinant proteins Methods in Enzymology 326 2000 271 304
8. Purification of His-tagged proteins
9. Affinity purification of a recombinant protein expressed as a fusion with the maltose-binding protein (MBP) tag
10. Purification of GST-tagged proteins
11. Protein Affinity Purification using Intein/Chitin Binding Protein Tags
12. Affinity Purification of Protein Complexes Using TAP Tags
13. Recombinant protein expression in baculovirus-infected insect cells
14. Small-scale Expression of Proteins in E. coli
15. Single Cell Cloning of a Stable Mammalian Cell Line
16. Explanatory Chapter: Troubleshooting protein expression: what to do when the protein is not soluble
17. Explanatory Chapter: Choosing the right detergent
18. Quantification of Protein Concentration using UV absorbance and Coomassie Dyes
19. One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE)