A sweet spot for molecular diagnostics: Coupling isothermal amplification and strand exchange circuits to glucometers

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Du, Yan ^a   Hughes, Randall A ^a   Bhadra, Sanchita ^a   Jiang, Yu Sherry ^a   Ellington, Andrew D ^a   Li, Bingling ^a,b  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^b CHANGCHUN INSTITUTE OF APPLIED CHEMISTRY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; BETA FRUCTOFURANOSIDASE; GLUCOSE;

CHEMISTRY; EBOLAVIRUS; GENETICS; NUCLEIC ACID AMPLIFICATION; SARS CORONAVIRUS;

BACTERIAL PROTEINS; BETA-FRUCTOFURANOSIDASE; EBOLAVIRUS; GLUCOSE; NUCLEIC ACID AMPLIFICATION TECHNIQUES; SARS VIRUS;

EID: 84930948453     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep11039     Document Type: Article

References (45)

1. Zaki, A. M., van Boheemen, S., Bestebroer, T. M., Osterhaus, A. D. M. E. & Fouchier, R. A. M. Isolation of a Novel Coronavirus from a Man with Pneumonia in Saudi Arabia. New Engl. J. Med. 367, 1814-1820 (2012).
2. Holmes, D. MERS-CoV enigma deepens as reported cases surge. Lancet 383, 1793-1793 (2014).
3. Cotten, M. et al. Transmission and evolution of the Middle East respiratory syndrome coronavirus in Saudi Arabia: a descriptive genomic study. Lancet 382, 1993-2002(2013).
4. Update: Recommendations for Middle East Respiratory Syndrome Coronavirus (MERS-CoV). Mmwr-Morbid Mortal. W. 62, 557-557 (2013).
5. Bialek, S. R. et al. First Confrmed Cases of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Infection in the United States, Updated Information on the Epidemiology of MERS-CoV Infection, and Guidance for the Public, Clinicians, and Public Health Authorities-May 2014. Mmwr-Morbid Mortal. W. 63, 431-436 (2014).
6. Coleman, C. M. & Frieman, M. B. Coronaviruses: Important Emerging Human Pathogens. J. Virol. 88, 5209-5212 (2014).
7. Corman, V. M. et al. Assays for laboratory confrmation of novel human coronavirus (hCoV-EMC) infections. Eurosurveill. 17, pii:20334 (2012).
8. Corman, V. M. et al. Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Eurosurveill.17, pii: 20285 (2012).
9. Fauci, A. S. An audience with-Anthony Fauci. Nat. Rev. Drug. Discov. 7, 12-12 (2008).
10. Corman, V. M. et al. Performance and clinical validation of the RealStar (R) MERS-CoV Kit for detection of Middle East respiratory syndrome coronavirus RNA. J. Clin. Virol. 60, 168-171(2014).
11. Compton, J. Nucleic-Acid Sequence-Based Amplifcation. Nature 350, 91-92 (1991).
12. Dean, F. B. et al. Comprehensive human genome amplifcation using multiple displacement amplifcation. Proc. Natl. Acad. Sci. USA 99, 5261-5266 (2002).
13. Hall, M. J., Wharam, S. D., Weston, A., Cardy, D. L. N. & Wilson, W. H. Use of signal-mediated amplifcation of RNA technology (SMART) to detect marine cyanophage DNA. Biotechniques 32, 604-6 (2002).
14. Walker, G. T. et al. Strand Displacement Amplifcation-an Isothermal, Invitro DNA Amplifcation Technique. Nucleic Acids Res. 20, 1691-1696 (1992).
15. Kurn, N. et al. Novel isothermal, linear nucleic acid amplifcation systems for highly multiplexed applications. Clin. Chem. 51, 1973-1981 (2005).
16. Crannell, Z. A., Castellanos, G. A., Irani, A., Rohrman, B., White, A. C. & Richard. K. R. Nucleic Acid Test to Diagnose Cryptosporidiosis: Lab Assessment in Animal and Patient Specimens. Anal. Chem. 86, 2565-2571 (2014).
17. Notomi, T. et al. Loop-mediated isothermal amplifcation of DNA. Nucleic Acids Res. 28, e63(2000).
18. Nagamine, K., Kuzuhara, Y. & Notomi, T. Isolation of single-stranded DNA from loop-mediated isothermal amplifcation products. Biochem. Bioph. Res. Co. 290, 1195-1198 (2002).
19. Li, B. L., Chen, X. & Ellington, A. D. Adapting Enzyme-Free DNA Circuits to the Detection of Loop-Mediated Isothermal Amplifcation Reactions. Anal. Chem. 84, 8371-8377 (2012).
20. Bhadra, S. et al. Real-time sequence-validated loop-mediated isothermal amplifcation assays for detection of Middle East respiratory syndrome coronavirus (MERS-CoV). PlosOne, just accepted (2015).
21. Jiang, Y. S. et al. Robust strand exchange reactions for the sequence-specifc, real-time detection of nucleic acid amplicons, Anal. Chem. 87, 3314-3320 (2015).
22. Xiang, Y. & Lu, Y. Using personal glucose meters and functional DNA sensors to quantify a variety of analytical targets. Nat. Chem. 3, 697-703 (2011).
23. Xiang, Y. & Lu, Y. An invasive DNA approach toward a general method for portable quantifcation of metal ions using a personal glucose meter. Chem. Commun. 49, 585-587(2013).
24. Xiang, Y. & Lu, Y. Using Commercially Available Personal Glucose Meters for Portable Quantifcation of DNA. Anal. Chem. 84, 1975-1980 (2012).
25. Zhou, Z. J., Xiang, Y., Tong, A. J. & Lu, Y. Simple and Efcient Method to Purify DNA-Protein Conjugates and Its Sensing Applications. Anal. Chem. 86, 3869-3875 (2014).
26. Liao, J. Y. & Li, H. Target-induced DNAzyme Cleavage Accompanying Bioactive Enzymatic Assembly with Glucometer Readout for Quantitative Monitoring of Lead Ion. Chem. Lett. 43, 1599-1600 (2014).
27. Joo, J. et al. A facile and sensitive method for detecting pathogenic bacteria using personal glucose meters. Sensor Actuat. B-Chem., 188, 1250-1254 (2013).
28. Wang, Q. et al. Multiplex detection of nucleic acids using a low cost microfuidic chip and a personal glucose meter at the point-of-care. Chem. Commun., 50, 3824-3826 (2014).
29. Hun, X., Xu, Y. Q., Xie, G. L. & Luo, X. L. Aptamer biosensor for highly sensitive and selective detection of dopamine using ubiquitous personal glucose meters. Sensor Actuat. B-Chem., 209, 596-601 (2015).
30. Xu, X. T., Liang, K. Y. & Zeng, J. Y. Portable and sensitive quantitative detection of DNA based on personal glucose meters and isothermal circular strand-displacement polymerization reaction. Biosens. Bioelectron., 64, 671-675 (2015).
31. WHO. Laboratory Testing for Middle East Respiratory Syndrome Coronavirus. Available:http://www.who.int/csr/disease/coronavirus-infections/WHO-interim-recommendations
32. M Citri, N. & Zyk, N. The interaction of penicillinase with penicillins I V. Structural aspects of catalytic and non-catalytic interactions. Biochimica Et Biophysica Acta 99, 427-441 (1965).
33. Kuramits, H. k. Characterization of Invertase Activity from Cariogenic Streptococcus mutans. J. Bacteriol. 115, 1003-1010 (1973).
34. Griessler, R., D'Auria, S., Tanfani, F. & Nidetzky, B. Termal denaturation pathway of starch phosphorylase from Corynebacterium callunae: Oxyanion binding provides the glue that efciently stabilizes the dimer structure of the protein. Protein Sci. 9, 1149-1161 (2000).
35. Zhu, J. B., Zhang, L. B., Li, T., Dong, S. J. & Wang, E. K. Enzyme-Free Unlabeled DNA Logic Circuits Based on Toehold-Mediated Strand Displacement and Split G-Quadruplex Enhanced Fluorescence. Adv. Mater. 25, 2440-2444 (2013).
36. Teichmann, M., Kopperger, E. & Simmel, F. C. Robustness of Localized DNA Strand Displacement Cascades. Acs Nano 8, 8487-8496 (2014).
37. Liu, M. et al. A DNA tweezer-actuated enzyme nanoreactor. Nat. Commun. 4, 2127 (2013).
38. Drosten, C. et al. Clinical features and virological analysis of a case of Middle East respiratory syndrome coronavirus infection. Lancet Infect. Dis. 13, 745-751 (2013).
39. Center for Dessease control and prevention: First Confrmed Cases of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Infection in the United States, Updated Information on the Epidemiology of MERS-CoV Infection, and Guidance for the Public, Clinicians, and Public Health Authorities, MMWR, 63, 431-436 (2014).
40. Sashikumar, R. & Kannan, R. Salivary glucose levels and oral candidal carriage in type II diabetics. Oral Surg. Oral Med. Oral Pathol. Oral. Radiol. Endod. 109, 706-711(2010).
41. Bausch, D. G. et al. Assessment of the risk of Ebola virus transmission from bodily fuids and fomites. J. Infect. Dis. 196, S142-S147, doi:10.1086/520545 (2007).
42. Formenty, P. et al. Detection of Ebola virus in oral fuid specimens during outbreaks of Ebola virus hemorrhagic fever in the republic of Congo. Clin. Infect. Dis. 42, 1521-1526 (2006).
43. Richardson, S. M., Wheelan, S. J., Yarrington, R. M. & Boeke, J. D. GeneDesign: Rapid, automated design of multikilobase synthetic genes. Genome Res. 16, 550-556 (2006).
44. Gibson, D. G. et al. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat. Methods 6, 343-341 (2009).
45. Cox, J. C., Lape, J., Sayed, M. A. & Hellinga, H. W. Protein fabrication automation. Protein Science 16, 379-390 (2007).